Chemistry and Industry of Forest Products ›› 2023, Vol. 43 ›› Issue (1): 120-126.doi: 10.3969/j.issn.0253-2417.2023.01.015
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Jia FU1, Yujian WU1, Zhiming LIU2, Haiying WANG1,*()
Received:
2021-12-17
Online:
2023-02-28
Published:
2023-02-28
Contact:
Haiying WANG
E-mail:haiyingwang908@126.com
CLC Number:
Jia FU, Yujian WU, Zhiming LIU, Haiying WANG. Chemical Constituents Analysis and Process Optimization of Ethanol Extract from Mulberry Leaves[J]. Chemistry and Industry of Forest Products, 2023, 43(1): 120-126.
Table 1
GC-MS analysis of absolute ethanol extract from mulberry leaves"
序号 No. | 保留时间/min retention time | 化合物 compound | 分子式 molecular | GC含量/% GC content | 匹配度/% matching degree |
1 | 3.843 | 1, 2-15, 16-二环氧十六烷 1, 2-15, 16-diepoxyhexadecane | C16H30O2 | 6.57 | 71.90 |
2 | 5.755 | α, β-二甲基苯乙醇 α, β-dimethyl phenylethanol | C10H14O | 24.78 | 83.40 |
3 | 6.170 | 对二甲苯 p-xylene | C8H10 | 10.23 | 69.80 |
4 | 8.013 | 1-(乙烯氧基)-十八烷 1-(ethenyloxy)-octadecane | C20H40O | 3.06 | 66.30 |
5 | 8.123 | 1-(3, 5-二甲基-1-金刚烷基)氨基脲 1-(3, 5-dimethyl-1-adamantanoyl)semicarbazide | C14H23N3O2 | 0.68 | 62.90 |
6 | 8.899 | 1, 2-二乙苯 1, 2-diethyl-benzene | C10H14 | 10.31 | 83.00 |
7 | 9.001 | 1, 4-二乙苯 1, 4-diethyl-benzene | C10H14 | 4.03 | 83.60 |
8 | 9.659 | 十一烷 undecane | C11H24 | 11.08 | 71.10 |
9 | 11.194 | 6-甲基十八烷 6-methyl-octadecane | C19H40 | 3.01 | 71.30 |
Table 2
GC-MS analysis of 70% ethanol extract from mulberry leaves"
序号 No. | 保留时间/min retention time | 化合物 compound | 分子式 molecular | GC含量/% GC content | 匹配度/% matching degree |
1 | 3.835 | 环乙烯巯基-5.α-雄甾烷-16-酮 cyclic ethylene mercaptole-5.α-androstan-16-one | C21H34S2 | 0.10 | 62.70 |
2 | 5.755 | α, β-二甲基苯乙醇 α, β-dimethyl phenylethanol | C10H14O | 0.45 | 84.40 |
3 | 6.853 | 1, 2-环戊二酮 1, 2-cyclopentanedione | C5H6O2 | 0.28 | 77.70 |
4 | 7.967 | 双(三甲基硅基)醚-2',6'-二羟基苯乙酮 bis(trimethylsilyl) ether-2', 6'-dihydroxyacetophenone | C14H24O3Si2 | 9.19 | 85.70 |
5 | 8.575 | 四甲基丁腈 tetramethylbutanedinitrile | C8H12N2 | 0.52 | 78.90 |
6 | 9.658 | 十一烷 undecane | C11H24 | 0.18 | 78.60 |
7 | 10.475 | 苯甲酸-2, 5-二(三甲基甲硅烷氧基)-三甲基甲硅烷基酯 benzoic acid-2, 5-bis(trimethylsiloxy)-, trimethylsilyl ester | C18H25N3OSi2 | 20.01 | 73.40 |
8 | 11.19 | 6-甲基-十三烷 6-methyl-tridecane | C14H30 | 0.28 | 73.50 |
9 | 12.961 | 十二甲基环己硅氧烷dodecamethyl-cyclohexasiloxane | C12H36O6Si6 | 8.57 | 94.30 |
10 | 17.028 | 3-三氟甲基苯甲酸-4-十六酯 3-trifluoromethylbenzoic acid-4-hexadecyl ester | C24H37F3O2 | 0.33 | 65.50 |
11 | 17.203 | [[4-[1, 2-双[(三甲基硅基)氧基]乙基]-1, 2-亚苯基]双(氧基)]双[三甲基]-硅烷 [[4-[1, 2-bis[(trimethylsilyl)oxy]ethyl]-1, 2-phenylene]bis(oxy)]bis[trimethyl]-silane | C20H42O4Si4 | 4.12 | 70.70 |
12 | 18.936 | 1, 1, 1, 5, 7, 7, 7-七甲基-3, 3-双(三甲基硅氧基)-四硅氧烷 1, 1, 1, 5, 7, 7, 7-heptamethyl-3, 3-bis(trimethylsiloxy)-tetrasiloxane | C13H40O5Si6 | 5.66 | 69.10 |
13 | 19.282 | 十五酸 pentadecanoic acid | C15H30O2 | 0.34 | 83.30 |
14 | 19.453 | 1, 2-苯二甲酸丁辛酯 1, 2-benzenedicarboxylic acid-butyl octyl ester | C20H30O4 | 0.15 | 76.20 |
15 | 20.289 | 正十六酸 n-hexadecanoic acid | C16H32O2 | 6.26 | 96.10 |
16 | 20.483 | 1, 1, 3, 3, 5, 5, 7, 7, 9, 9, 11, 11, 13, 13-十四甲基-七硅氧烷 1, 1, 3, 3, 5, 5, 7, 7, 9, 9, 11, 11, 13, 13-tetradecamethyl-heptasiloxane | C14H44O6Si7 | 5.54 | 74.90 |
17 | 21.905 | 苯乙酸-α,3, 4-三[(三甲基硅基)氧]-三甲基硅基酯 benzeneacetic acid-α, 3, 4-tris[(trimethylsilyl)oxy]-trimethylsilyl ester | C20H40O5Si4 | 5.79 | 69.30 |
18 | 22.175 | 十八酸 octadecanoic acid | C18H36O2 | 0.47 | 80.40 |
19 | 26.580 | 苯甲酸-2, 5-二(三甲基硅氧基)-三甲基硅基酯 benzoic acid-2, 5-bis(trimethylsiloxy)- trimethylsilyl ester | C16H30O4Si3 | 6.71 | 61.10 |
Table 4
Scheme and results of response surface test"
序号 No. | X1 乙醇体积分数/% ethanol volume fraction | X2 提取温度/℃ extraction temperature | X3 提取时间/h extraction time | 提取物得率/% extraction yield |
1 | 70 | 65 | 4 | 12.2 |
2 | 70 | 85 | 2 | 18.0 |
3 | 70 | 75 | 3 | 15.2 |
4 | 80 | 85 | 3 | 13.0 |
5 | 70 | 75 | 3 | 15.6 |
6 | 60 | 65 | 3 | 10.4 |
7 | 80 | 75 | 4 | 9.6 |
8 | 60 | 85 | 3 | 18.4 |
9 | 60 | 75 | 4 | 13.8 |
10 | 70 | 75 | 3 | 15.8 |
11 | 70 | 75 | 3 | 16.6 |
12 | 60 | 75 | 2 | 12.4 |
13 | 70 | 85 | 4 | 17.6 |
14 | 70 | 75 | 3 | 15.0 |
15 | 80 | 75 | 2 | 11.4 |
16 | 80 | 65 | 3 | 8.8 |
17 | 70 | 65 | 2 | 11.6 |
Table 5
Variance analysis of regression model"
方差来源 source of variance | 平方和 sum of squares | 自由度 degree of freedom | 均方 mean square | F值 F value | P值 P value | 显著性 significance |
模型 model | 139.62 | 9 | 15.51 | 53.71 | < 0.000 1 | ** |
X1 | 18.61 | 1 | 18.61 | 64.41 | < 0.000 1 | ** |
X2 | 72.00 | 1 | 72.00 | 249.26 | < 0.000 1 | ** |
X3 | 0.005 | 1 | 0.005 | 0.017 | 0.899 0 | |
X1X2 | 3.61 | 1 | 3.61 | 12.50 | 0.009 5 | ** |
X1X3 | 2.56 | 1 | 2.56 | 8.86 | 0.020 6 | * |
X2X3 | 0.25 | 1 | 0.25 | 0.87 | 0.383 2 | |
X12 | 38.40 | 1 | 38.40 | 132.94 | < 0.000 1 | ** |
X22 | 0.003 8 | 1 | 0.003 8 | 0.013 | 0.912 0 | |
X32 | 2.83 | 1 | 2.83 | 9.80 | 0.016 6 | * |
残差 residual | 2.02 | 7 | 0.29 | |||
失拟 lack of fit | 0.47 | 3 | 0.16 | 0.40 | 0.758 9 | |
误差 pure error | 1.55 | 4 | 0.39 | |||
总计 cor total | 141.64 | 16 |
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