白桦树皮中白桦脂醇的抗氧性测定研究

›› 2008, Vol. 28 ›› Issue (5): 105-108.

• 研究报告 • Previous Articles Next Articles

Study on Determining the Antioxidant Activity of Betulin from Betula Bark

SUN Hong¹, ZHANG Ze², LIU Lin-yu¹

1. Chemistry and Chemical Engineering College, Qiqihar University, Qiqihar 161006, China;2. Qiqihar Environment Monitoring Center, Qiqihar 161005, China

Received:2007-10-10 Revised:1900-01-01 Online:2008-10-30 Published:2008-10-30

Abstract

Abstract: A new method of determining the antioxidant activity of betulin from betula bark is established.In HCl-NaOAc(pH value 4.20) buffer solution, the hydroxyl free radical from Fenton reaction is captured by excess KI and the released I₃^- combines with Rhodamine B(RhB λ_max=556nm) to form associated compound, so the absorbance at maximum wavelength decreases. The concentration of hydroxyl free radical(calculated by the concentration of hydrogen peroxide) is proportional to the decreased absorbance of the RhB system in the range of 0.14-0.68mg/L. Based on this fact, the antioxidatant activities of betulin and ascorbic acid are measured with satisfactory results.

Key words: betulin, spectrophotometry, antioxidants, Rhodamine dye, betula bark

CLC Number:

TQ351.0

SUN Hong;ZHANG Ze;LIU Lin-yu. Study on Determining the Antioxidant Activity of Betulin from Betula Bark[J]. , 2008, 28(5): 105-108.

[1]	TAO Ran, WANG Chengzhang, ZHANG Changwei, ZHOU Hao, CHEN Hongxia. Synthesis and Antitumor Activity of Selective Oxidation Products of Betulin [J]. Chemistry and Industry of Forest Products, 2018, 38(4): 47-51.
[2]	DONG Shi-hui, CUI Ming-jin, LIU Wei, ZHU Di, DU Yi-bo, WANG Ting. Preparation and Water Solubility of Betulinic Acid/Diethylenetriamine-β-cyclodextrin Complex [J]. Chemistry and Industry of Forest Products, 2016, 36(2): 30-36.
[3]	MA Jian-feng, YANG Shu-min, TIAN Gen-lin, LIU Xing-e, XU Feng. Application of UV-microspectrophotometry on Lignin Topochemistry in Plant Cell Wall [J]. Chemistry and Industry of Forest Products, 2016, 36(1): 147-154.
[4]	XIE Yang, LI Peng, YANG Fan, FU Dong, SUI Xin, ZHANG Hong-wei. Preparation of Betulinic Acid by Catalytic Oxidation of Betulin Using Alumina-potassium Dichromate as Catalyst [J]. Chemistry and Industry of Forest Products, 2015, 35(5): 111-115.
[5]	LUO Sha-man;DING Wei-min;YU Tao;WANG Yang. Flooding Extraction of Betulin [J]. , 2013, 33(1): 54-58.
[6]	SUI Ruo-jun;ZHANG Xiao-li;FANG Gui-zhen;FU Yu-jie. Syntheses of Tert-butyl Esters of 3,28-Di-o-succinyl-aminoacyl-betulin and Their Effects on the Proliferation and Apoptosis of HepG-2 [J]. , 2013, 33(1): 59-63.
[7]	YANG Shu-min;REN Hai-qing;FEI Ben-hua;JIANG Ze-hui. Lignin Distribution in Cell Wall of Bamboo Culms (Phyllostachys pubescens and Pseudosasa amabilis) [J]. , 2010, 30(5): 19-24.
[8]	DING Wei-min;ZHOU Dan;YU Tao;HUANG Jing-shu;YAN Xiu-feng;WANG Yang. Optimization of Ultrasound-assisted Extraction of Betulin Using Uniform Design [J]. , 2010, 30(3): 78-82.
[9]	YANG Ling;WANG Zhen-yu;. Optimization of the Processing Parameters for Extraction of Antioxidants from Blackberry Using Response Surface Methodology [J]. , 2010, 30(1): 67-72.
[10]	LÜKun;TIAN Shui-qing;FANG Gui-zhen. Synthesis of 3,28-Di-O-glutaryl-betulin and Analysis of Its Solubility Properties [J]. , 2009, 29(1): 87-90.
[11]	DING Wei-min;WANG Yang;ZHOU Dan;WANG Qian;YAN Xiu-feng;YU Tao. Optimization on Microwave-assisted Extraction of Betulin in Betula platyphylla Suk. Using Uniform Design [J]. , 2007, 27(S1): 69-72.
[12]	TIAN Shui-qing;Lü Kun;FANG Gui-zhen;HAN Shi-yan;AI Qing. Synthesis of 3,28-Di-O-succinyl-betulin [J]. , 2007, 27(S1): 77-80.
[13]	WANG Shi-fa;ZHANG Ming-guang;LI Yan-ping. Study on Extraction and Purification of Betulin from Birch Bark [J]. , 2007, 27(6): 22-26.
[14]	WU Dong-mei;CHEN Jia-hong;WANG Yong-mei;WU Zai-song . Study on Quantitative Determination of 1,2,3-Trimethoxybenzene [J]. , 2007, 27(6): 95-98.
[15]	DING Wei-min;WANG Yang;YAN Xiu-feng;YU Tao. Optimization of the Extraction of Betulin by Supercritical CO₂ Using Uniform Design [J]. , 2007, 27(3): 63-66.

Study on Determining the Antioxidant Activity of Betulin from Betula Bark

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments