Abstract: The extractions of total flavonoid and juglone ffrom leaves, bark and green peel of Juglans mandshurica Maxim. trees were carried out by three extracting methods respectively, including common method, reflux method and ultrasonic method.UV spectroscopy was used for determining the amount of total flavonoid, and HPLC was used for determining the amount of juglone. Results showed that amounts of total flavonoid and juglone are in the order of:ultrasonic method>common method>reflux method. Furthermore amounts of total flavonoid extracted from three materials by all three method showed the order of leaves>bark>green peel. Amounts of juglone extracted from three materials by all three method showed the order of:green peel>leaves>bark. The amounts of total flavonoid and juglone extracted by ultrasonic method are:5.00% and 0.00209% for leaves, 4.17% and 0.00131% for bark, 3.05% and 0.002 29% for green peel, respectively.

Key words: Juglans mandshurica Maxim., total flavonoid, juglonoid