神秘果叶挥发油化学成分分析及抗菌、抗肿瘤活性

doi:10.3969/j.issn.0253-2417.2014.01.021

Abstract

Abstract: The aim of this study was to determine the chemical composition of Synsepalum dulcificum leaf essential oil obtained by steam distillation. The in vitro antibacterial activity and antitumoral activity of this essential oil was characterized. Sixty-eight compositions were separated. Among them 44 components were identified and represented 92.14% of the total detected constituents. The major chemical compounds of leaf essential oil were spathulenol (24.194%), limonene (15.805%), diisooctyl phthalate (12.402%), dibutyl phthalate (10.326%), palmitic acid (4.865%) and linalool (2.139%). The result of the antimicrobial assay showed that the essential oil displayed varying degrees of antibacterial activity against all tested bacterial except for Pseudomonas aeruginosa, along with minimum inhibition concentration(MIC)values from 39.06 to 252.15 mg/L. In addition, the antitumoral activity using MTT assay of the leaf essential oil exhibited this oil was effective against human K562 cancer cell line in a dose dependent manner. Its inhibition concentration 50% (IC₅₀) value was 13.5 mg/L.

Key words: Synsepalum dulcificum leaf, essential oil, chemical composition, antibacterial activity, antitumoral activity

CLC Number:

TQ351

LU Sheng-lou LU Sheng-lou, LIU Hong, CHEN Guang-ying, HAN Chang-ri, ZANG Wen-xia. Chemical Composition of Leaf Essential Oil of Synsepalum dulcificum and Evaluation of Its Antibacterial and Antitumoral Activities in vitro[J]. Chemistry and Industry of Forest Products, 2014, 34(1): 121-127.

References

[1] TEPE B,DONMEZ E,UNLU M,et al.Antimicrobial and antioxidative activities of the essential oils and methanol extracts of Salvia cryptantha (Montbret et Aucher ex Benth) and Salvia multicaulis (Vahl)[J] .Food Chemistry,2004,84(4):519-525.
[2] 卢圣楼,刘红,江虹,等.微波辅助提取HPLC 法测定神秘果种子中齐墩果酸[J] .湖北农业科学,2012,51(4):816819,830.
[3] 卢圣楼,刘红,曹佳佳,等.神秘果中齐墩果酸和五环三萜含量的测定[J] .食品工业,2012,33(3):130133.
[4] SUN H J,CUI M L,MA Biao,et al.Functional expression of the taste-modifying protein,miraculin,in transgenic lettuce[J] .FEBS Letters,2006,580(2):620626.
[5] 齐赛男,贾桂云,雷鹏,等.神秘果种子挥发油化学成分的气相色谱质谱分析[J] .海南师范大学学报:自然科学版,2012,25(1):7376.
[6] WANG H M,CHOU Y T,HONG Z L,et al.Bioconstituents from stems of Synsepalum dulcificum Daniell (Sapotaceae) inhibit human melanoma proliferation,reduce mushroom tyrosinase activity and have antioxidant properties[J] .Journal of the Taiwan Institute of Chemical Engineers,2011,42(2):204211.
[7] 黄巨波,刘红,卢圣楼,等.神秘果种子蛋白质的提取与降糖效用研究[J] .天然产物研究与开发,2012,24(10):14411443,1347.
[8] WONG J M,KERN M.Miracle fruit improves sweetness of a low-calorie dessert without promoting subsequent energy compensation[J] .Appetite,2011,56(1):163166.
[9] 黄巨波,刘红,祁海兰,等.神秘果混合果粉营养成分及其抗疲劳和免疫作用[J] .中国实验方剂学杂志,2012,18(14):195198.[ZK)]
[10] TEIXEIRA B,MARQUES A,RAMOS C,et al.European pennyroyal (Mentha pulegium) from Portugal:Chemical composition of essential oil and antioxidant and antimicrobial properties of extracts and essential oil[J] .Industrial Crops and Products,2012,36(1):8187.
[11] DUNG N T,KIM J M,KANG S C.Chemical composition,antimicrobial and antioxidant activities of the essential oil and the ethanol extract of Cleistocalyx operculatus (Roxb.) Merr and Perry buds[J] .Food and Chemical Toxicology,2008,46(12):36323639.
[12] MOSMANN T.Rapid colorimetric assay for cellular growth and survival:Application to proliferation and cytotoxicity assays[J] .Journal of Immunological Methods,1983,65(1/2):5563.
[13] DE SOUSA A C,ALVIANO D S,BLANK A F,et al.Melissa officinalis L.essential oil:Antitumoral and antioxidant activities[J] .Journal of Pharmacy and Pharmacology,2004,56(5):677681.
[14] JOSHI S C,VERMA A R,MATHELA C S.Antioxidant and antibacterial activities of the leaf essential oils of Himalayan Lauraceae species[J] .Food and Chemical Toxicology,2010,48(1):3740.
[15] SHAABAN H A E,EL-GHORAB A H,SHIBAMOTO T.Bioactivity of essential oils and their volatile aroma components:Review[J] .Journal of Essential Oil Research,2012,24(2):203212.
[16] DAMBOLENA J S,LOPEZ A G,CANEPA M C,et al.Inhibitory effect of cyclic terpenes (limonene,menthol,menthone and thymol) on Fusarium verticillioides MRC 826 growth and fumonisin B1 biosynthesis[J] .Toxicon,2008,51(1):3744.
[17] FRIGHETTO N,DE OLIVEIRA J G,SIANI A C,et al.Lippia alba Mill N.E.Br.(Verbenaceae) as a source of linalool[J] .Journal of Essential oil Research,1998,10(5):578580.
[18] BATISTA P A,WERNER M F,OLIVEIRA E C,et al.Evidence for the involvement of ionotropic glutamatergic receptors on the antinociceptive effect of (-)-linalool in mice[J] .Neuroscience Letters,2008,440(3):299303.
[19] ELISABETSKY E,BRUM L F,SOUZA D O.Anticonvulsant properties of linalool in glutamate-related seizure models[J] .Phytomedicine,1999,6(2):107113.
[20] SUGAWARA Y,HARA C,TAMURA K,et al.Sedative effect on humans of inhalation of essential oil of linalool:Sensory evaluation and physiological measurements using optically active linalools[J] .Analytica Chimica Acta,1998,365(1/2/3):293299.
[21] COX S D,MANN C M,MARKHAM J L,et al.Determining the antimicrobial actions of tea tree oil[J] .Molecules,2001,6(2):8791.
[22] CHEN Xiao-qiang,ZHANG Ying,ZU Yuan-gang,et al.Composition and biological activities of the essential oil from Schisandra chinensis obtained by solvent-free microwave extraction[J] .LWT-Food Science and Technology,2011,44(10):20472052.
[23] CARDILE V,RUSSO A,FORMISANO C,et al.Essential oils of salvia bracteata and salvia rubifolia from Lebanon:Chemical composition,antimicrobial activity and inhibitory effect on human melanoma cells[J] .Journal of Ethnopharmacology,2009,126(2):265272.

Chemical Composition of Leaf Essential Oil of Synsepalum dulcificum and Evaluation of Its Antibacterial and Antitumoral Activities in vitro

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LIU Yijun, YUAN Yuan, LIU Yangyang, ZHANG Fan, LI Jihua. Effects of Different Drying Methods on Physicochemical Properties and Essential Oil Quality of Melaleuca alternifolia Cheel [J]. Chemistry and Industry of Forest Products, 2019, 39(4): 77-82.
[2]	LI Kun, ZHANG Wenwen, LI Kai, XU Juan, MA Jinju, LIU Lanxiang, ZHANG Hong. Preparative Resolution and Stereochemical Composition of Aleuritic Acid Optical Isomers [J]. Chemistry and Industry of Forest Products, 2019, 39(3): 72-78.
[3]	ZHENG Hongfu, LIAO Shengliang, FAN Guorong, WANG Zongde, CHEN Shangxing, SI Hongyan. Extraction of Cinnamomum camphora Essential Oil by Steam Distillation and Its Antifungal Activity [J]. Chemistry and Industry of Forest Products, 2019, 39(3): 108-114.
[4]	ZHANG Zuohui, QIAN Xinyi, HUANG Guangzhi, BI Liangwu. Component and Antibacterial Activity of Different Polar Parts of Cinnamon and Homologous Root Bark [J]. Chemistry and Industry of Forest Products, 2019, 39(3): 115-122.
[5]	XUE Xingying, ZHOU Hao, ZHANG Changwei, CHEN Hongxia, WANG Chengzhang. Preparation, Antibacterial Activity and Preservation of Lacquer Wax Composite Coating Preservative [J]. Chemistry and Industry of Forest Products, 2019, 39(3): 123-128.
[6]	XU Shichao, ZENG Xiaojing, DONG Huanhuan, ZHAO Zhendong. Research Progress in Development and Application of Terpenoid Botanical Herbicides [J]. Chemistry and Industry of Forest Products, 2019, 39(2): 1-8.
[7]	XU Shichao, DONG Huanhuan, ZENG Xiaojing, ZHAO Zhendong. Research Progress in Screening and Bioactivity of Terpenoid Botanical Pesticides [J]. Chemistry and Industry of Forest Products, 2019, 39(1): 1-12.
[8]	WANG Xingda, LI Lei, HAN Yongping. Synthesis and Antibacterial Activity of Derivatives of Rhein [J]. Chemistry and Industry of Forest Products, 2018, 38(2): 105-111.
[9]	CHEN Chao, YU Yuxiang, HAN Liping, XING Jingchen, CHANG Jianmin. Antimidew and Antibacterial Characteristics of Larix Bio-oil [J]. Chemistry and Industry of Forest Products, 2018, 38(1): 81-86.
[10]	CHEN Hongxia, KANG Xiutang, YOU Longjie, WANG Chengzhang, YE Jianzhong. Chemical Component and Antibacterial Activity of Essential Oil from Myanmar Pterocarpus macrocarpus [J]. Chemistry and Industry of Forest Products, 2017, 37(6): 65-72.
[11]	ZHU Shouji, YANG Xuebing, XU Shichao, ZHAO Zhendong, JIANG Jianxin. Synthesis and Antibacterial Activity of Bis-alkanoyl-amines Heterocyclic Derivatives of cis-1,8-p-Menthane Diamine [J]. Chemistry and Industry of Forest Products, 2017, 37(2): 79-84.
[12]	TAO Ran, WANG Chengzhang, YE Jianzhong, ZHOU Hao, CHEN Hongxia. Antibacterial Activity of Ginkgo biloba Leaves Polyprenols Nanoemulsion in Combination with Antibiotics [J]. Chemistry and Industry of Forest Products, 2017, 37(1): 81-86.
[13]	TAO Ran, WANG Cheng-zhang, YE Jian-zhong, ZHOU Hao, CHEN Hong-xia. Synthesis and Antibacterial Activity of Nitrogenated and Haloid Derivatives of Ginkgo Biloba Leaves Polyprenol [J]. Chemistry and Industry of Forest Products, 2016, 36(6): 29-34.
[14]	RUI Jian, YANG Jin-lai, CAI Tao, XU Xu, WANG Shi-fa. Synthesis of Isolongifolanyl Thiazole Compounds and Their Antibacterial and Antitumor Activities [J]. Chemistry and Industry of Forest Products, 2016, 36(6): 41-48.
[15]	CUI He-shuai, YANG Shu-min, LIU Xing-e, MA Jian-feng, TIAN Gen-lin. Chemical Composition and Lignin Distribution of Salix integra [J]. Chemistry and Industry of Forest Products, 2016, 36(5): 120-126.