高速及高分辨率原子力显微镜用于研究Trichodermareesei纤维素酶在结晶纤维素上的行为

doi:10.3969/j.issn.0253-2417.2014.03.022

Abstract

Abstract: The advantages of the atomic force microscopy and high speed atomic force microscopy on studying the dynamics of bio-macromolecule and the cellulytic enzyme system of Trichoderma reesei were presented. The results of high speed atomic force microscopy and high resolution atomic force microscopy in investigating the actions of Cel7A(TrCel7A), Cel6A(TrCel6A) and Cel7B(TrCel7B) of Trichoderma reesei on crystalline cellulose were reviewed in detail. The conclusion reveals that the loading of crystalline cellulose into catalytic domain and the hydrolysis of the glucosidic bond are prerequisite for the movement of Cel7A; increasing the hydrophobic area of crystalline cellulose and the combination of Cel6A and Cel7A enhance the hydrolytic rate of crystalline cellulose; Cel7B makes crystalline cellulose swelling. And it is prospective that HP-AFM will play an important role in elucidating the acting mechanism of cellulase on crystalline cellulose.

Key words: high speed atomic force microscopy, cellullase, crystalline cellulose

CLC Number:

TQ35
Q939.97

WANG Fang-fang, HUANG Feng. The Action of Trichoderma reesei Cellulases on Crystalline Cellulose with High Speed and High Resolution Atomic Force Microscopy[J]. Chemistry and Industry of Forest Products, 2014, 34(3): 130-134.

References

[1] WANG Jing-peng,QUIRK A,LIPKOWSKI J,et al.Real-time observation of the swelling and hydrolysis of a single crystalline cellulose fiber catalyzed by cellulase 7B from Trichoderma reesei[J].Langmuir,2012,28(25):9664-9672.
[2] UCHIHASHI T,KODERA N,ANDO T.Guide to video recording of structure dynamics and dynamic processes of proteins by high-speed atomic force microscopy[J].Nature Protocols,2012,7(6):1193-1206.
[3] ANDO T.High-speed atomic force microscopy coming of age[J].Nanotechnology,2012,23(6):062001.
[4] KATAN A J,DEKKER C.High-speed AFM reveals the dynamics of single biomolecules at the nanometer scale[J].Cell,2011,147(5):979-982.
[5] IINO R,UCHIHASHI T,ANDO T,et al.Direct observation of rotary catalysis of rotorless F1-ATPase by high-speed atomic force microscopy[J].Biophysical Journal,2012,102(3):600.
[6] ANDO T,UCHIHASHI T,FUKUMA T.High-speed atomic force microscopy for nano-visualization of dynamic biomolecular processes[J].Progress in Surface Science,2008,83(7/8/9):337-437.
[7] HUMPHRIS A D L,MILES M J,HOBBS J K.A mechanical microscope:High-speed atomic force microscopy[J].Applied Physics Letters,2005,86(3):034106-034106-3.
[8] MARTINEZ D,BERKA R M,HENRISSAT B,et al.Genome sequencing and analysis of the biomass-degrading fungus Trichoderma reesei (syn.Hypocrea jecorina)[J].Nature Biotechnology,2008,26(5):553-560.
[9] VIIKARI L,ALAPURANEN M,PURANEN T,et al.Thermostable enzymes in lignocellulose hydrolysis[J].Biofuels,2007,108:121-145.
[10] IGARASHI K,KOIVULA A,WADA M,et al.High speed atomic force microscopy visualizes processive movement of Trichoderma reesei cellobiohydrolase I on crystalline cellulose[J].Journal of Biological Chemistry,2009,284(52):36186-36190.
[11] LIU Yu-san,BAKER J O,ZENG Yi-ning,et al.Cellobiohydrolase hydrolyzes crystalline cellulose on hydrophobic faces[J].Journal of Biological Chemistry,2011,286(13):11195-11201.
[12] IGARASHI K,UCHIHASHI T,KOIVULA A,et al.Traffic jams reduce hydrolytic efficiency of cellulase on cellulose surface[J].Science,2011,333(6047):1279-1282.
[13] PETER J,GUNNAR H,LARS W.The Physical Action of Cellulases Reavealed by a Quartz Crystal Microbalance Study Using Ultrathin Cellulose Films and Pure Cellulases[J].Biomacromolecules,2008,9(1):249-254.

[1]	LI Yang, ZHU Jin-ke, LI Lian-jie, JIANG Deng-gao. Preparation and Application of Tripeptide-cellulose Ester [J]. Chemistry and Industry of Forest Products, 2016, 36(2): 71-78.
[2]	ZHANG Hao, PU Jun-wen, HONG Liang, ZHU Ming. Reduction of Free Formaldehyde Emission from Urea-formaldehyde Resin Adhesive by Use of Modified Nanocrystalline Cellulose [J]. Chemistry and Industry of Forest Products, 2016, 36(1): 99-104.
[3]	ZHANG Jing-qiang, LI Qing-chun, LIN Lu. Effect of Decrystallization of Cellulose on Adsorbing Cellulase [J]. Chemistry and Industry of Forest Products, 2016, 36(1): 105-111.
[4]	LU Yan-feng, WU Geng-feng, ZENG Chang-wei, LI Tao, LÜ Jian-hua, CHEN Yan-dan, HUANG Biao. In-situ Synthesis and Catalytic Properties of Carbon-based Phosphotungstic Acid [J]. Chemistry and Industry of Forest Products, 2015, 35(4): 48-52.
[5]	WU Yan-jiao, LI Wei, WU Qiong, ZHAO Xin, ZHANG Shuang-shuang, LIU Shou-xin. Preparation of Carbon Sphere-activated Carbon Composite Materials from Microcrystalline Cellulose via Hydrothermal Carbonization [J]. Chemistry and Industry of Forest Products, 2015, 35(3): 49-54.
[6]	YAO Wen-run, XU Qing-hua, JIN Li-qiang, CHENG Zheng-liang, GAO Yang. Effects of TEMPO/NaBr/NaClO-oxidation of Nanocrystalline Cellulose on Its Properties [J]. Chemistry and Industry of Forest Products, 2015, 35(2): 31-37.
[7]	HU Yi-ming, JIANG Jian-chun, SUN Yun-juan, YANG Zhong-zhi. Interaction during the Pyrolysis Process of Cellulose and Hemicellulose [J]. Chemistry and Industry of Forest Products, 2014, 34(4): 1-8.
[8]	SHE Ying;ZHANG Hao;SONG Shu-ping;CHEN He-yu;PU Jun-wen. Wetting Property of Modified Nanocrystalline Cellulose on Waterborne Polyurethane [J]. , 2013, 33(2): 77-80.
[9]	REN Ling-bing;CAO Qing;XIE Xiao-ling. Hydrolysis Kinetics of Microcrystalline Cellulose Catalyzed by Fe³⁺ and Dilute Hydrochloric Acid [J]. , 2012, 32(4): 117-122.
[10]	LIU Zhi-ming;XIE Cheng;FANG Gui-zhen;BU Liang-xiao;LIU Li-yang. Preparation Optimization and Morphology Analysis of Nanocrystalline Cellulose from Reed Pulp [J]. , 2011, 31(6): 87-90.
[11]	QU Bao-xue;QIN Te-fu;LI Yan;CHU Fu-xiang. Study on Cyanoethylation of Microcrystalline Cellulose and Its Reaction Kinetics [J]. , 2010, 30(5): 5-11.
[12]	LI Hong-zhen;FANG Gui-zhen;LI Jun-ye;MA Ying-mei;MA Yan-li;JIN Zhong-ling. Synthesis of Polyethyleneimine-microcrytalline Cellulose and Its Adsorption Character for Bilirubin [J]. , 2009, 29(5): 99-103.
[13]	WANG Xian-ling;FANG Gui-zhen. Influence on Structure and Oxidation Reactivity of Microcrystalline Cellulose by Different Activation Methods [J]. , 2007, 27(3): 67-71.
[14]	YUAN Xiao-ping;DING En-yong. Preparation of Nano-crystalline Cellulose/Polyethylene Glycol Solid-solid Phase Transition Material and Its Energy Storage Characteristics [J]. , 2007, 27(2): 67-70.
[15]	GUO Rui;DING En-yong. Viscosity Behavior and Spectral Characteristics of Nanocrystalline Cellulose [J]. , 2006, 26(04): 54-56.

The Action of Trichoderma reesei Cellulases on Crystalline Cellulose with High Speed and High Resolution Atomic Force Microscopy

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments