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Chemistry and Industry of Forest Products ›› 2015, Vol. 35 ›› Issue (3): 43-48.doi: 10.3969/j.issn.0253-2417.2015.03.008

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Chemical Association between Mannose Units and Lignin in Plant Cell Wall

SHI Chao-qun1, XIE Yi-min1,2, WANG Peng1,2   

  1. 1. School of Pulping &Papermaking Engineering, Hubei University of Technology, Wuhan 430068, China;
    2. Hubei Provincial Key Laboratory of Green Materials for Light Industry, Hubei University of Technology, Wuhan 430068, China
  • Received:2014-03-24 Online:2015-06-25 Published:2015-07-03

Abstract: In order to elucidate the chemical association between hemicellulose and lignin in gymnosperm, the linkage between sugar units in hemicellulose and phenylpropane substructure of lignin was analyzed from the perspective of mannose. The mannan precursor D-13C6-mannose, exogenous lignin precursor coniferin, and the inhibitor of phenylalanine ammonia-lyase, were injected into a living ginkgo tree (Ginkgo biloba L.) to trace the mannose in the cell wall by 13C and meanwhile inhibit the conversion of mannose to lignin. The results indicate that the mannose units of lignin-carbohydrate complexes (LCC) in newly-formed xylem are labeled successfully by 13C isotope. Moreover, the metabolism of ginkgo tree has not been affected obviously by the addition of the precursors and inhibitor. The specifically 13C-enriched LCCs were isolated from the newly-formed xylem and further hydrolyzed by cellulase and hemicellulase to get enzyme-degraded LCCs (EDLCCs). The analyses of FT-IR and 13C NMR of EDLCCs indicate that the C-6 position of mannose unit in hemicellulose and α-carbon of lignin side chain is linked by benzyl ether bond.

Key words: lignin-carbohydrate complexes, hemicellulose, mannose, 13C tracer, benzyl ether linkage

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