Abstract: Dihydromyricetion(DMY), which was abundant and easily extracted from the leaves of Ampelopsis grossedentata, was used as the semisynthetic sources for myricetin(MYR). The structure of MYR was characterized by IR, ¹H NMR and ¹³C NMR. Based on the results of the single factor experiments, response surface methodology(RSM) was applied to further optimize the synthesis conditions with pyridine volume fraction, and temperature, solid-liquid-ratio as the impact factors and MYR yield as the response value. The most suitable synthesis conditions of MYR were determined as follows: pyridine volume fraction 66.6%, temperature 90.4 ℃ and solid-liquid ratio 20.88(g:L). The MYR yield was 76.63%, and the relative error with the predictive value of 76.48% was 0.19%.

Key words: dihydromyricetion, semisynthetic, myricetin, response surface methodology