双水相木聚糖酶水解体系制备木二糖及其纯化

doi:10.3969/j.issn.0253-2417.2015.05.015

Abstract

Abstract: Xylobiose was prepared by xylanase hydrolysis of birchwood xylan in the PEG6000/sodium citrate aqueous two-phase system (ATPS). The optimized conditions for enzymatic hydrolysis in ATPS were birchwood xylan 10 g/L, xylanase 20 IU/g (calculated by the mass of xylan), 50 ℃ and pH 5.6. The enzymatic hydrolysis yield of xylobiose was 13.8% for 8h. The recoveries of xylanase and xylobiose were 66.4% and 87.2%, respectively. Then, purification of xylobiose was carried out on Sephadex LH-20 with using degassed high purified water as eluent. The optimized conditions for purification of xylobiose were column temperature 35 ℃, 0.1 mL/min and sample volume 0.3 mL. After purification, the purity of xylobiose was improved to 98.5% under the optimized conditions.

Key words: xylanase, aqueous two-phase system, enzymatic hydrolysis, gel chromatography, xylobiose

CLC Number:

TQ35
Q556

LI Xin, GU Xi-mei, SHENG Ao-nan, ZHANG Li, CHEN Xiao-pei, YONG Qiang. Preparation of Xylobiose by Xylanase Hydrolysis in Aqueous Two-phase System and Its Purification[J]. Chemistry and Industry of Forest Products, 2015, 35(5): 93-97.

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Preparation of Xylobiose by Xylanase Hydrolysis in Aqueous Two-phase System and Its Purification

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