漆树木材中漆黄素的分离及其抑制酪氨酸酶活性的研究

doi:10.3969/j.issn.0253-2417.2015.06.017

Abstract

Abstract: The Toxicodendron vernicifluum (Stokes) F.A.Barkl.woods were extracted with 70 % ehanol by decompressing inner ebullition method.The compound with the purity over 70 % was obtained through the extraction by rapid isolation of extracts with ethyl acetate and middle pressure chromatography.Then the compound was separated by HPLC to obtain monomeric compound.The compound was identified as fisetin by IR, MS and NMR.The inhibitories of fisetin on the activities of monophenolase and diphenolase contained in tyrosinase were studied.The results showed that fisetin could potently inhibit the generation of dopachrome.The half inhibitory concentration of monophenolase was 69.46 mg/L.The inhibitory of fisetin against monophenolase exhibited retardation effect.Fisetin with0.524 mmol/L could extend the lag time from 1 min to 11.2 min.Fisetin functioned as a competitive inhibitor against diphenolase by Lineweaver-Burk plots.

Key words: Toxicodendron vernicifluum (Stokes)F.A.Barkl., fisetin, middle pressure chromatography, tyrosinase, inhibition

CLC Number:

TQ351

CHEN Hong-xia, WANG Cheng-zhang, YE Jian-zhong, ZHOU Hao, TAO Ran. Separation of Fisetin from Toxicodendron vernicifluum (Stokes) F.A.Barkl.Woods and Its Anti-tyrosinase Activity[J]. Chemistry and Industry of Forest Products, 2015, 35(6): 101-107.

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Separation of Fisetin from Toxicodendron vernicifluum (Stokes) F.A.Barkl.Woods and Its Anti-tyrosinase Activity

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