›› 2000, Vol. 20 ›› Issue (2): 23-26.
• 研究报告 • Previous Articles Next Articles
LEI Fei-hou
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Abstract: A method for immobilization of polyphenol oxidases (PPO) was introduced.The PPO was immobilized on the coordinated polymer chitosan Cu2+.The results showed that optimum pH value of immobilization PPO was 6.24.The activity of immobilization PPO was retained 31.1% in 70℃ buffer solution for 25 minutes, much higher than that of free PPO.Besides,inhibition of substrate,reusability and Michaelis constant were discussed and showed that the method of immobilization of enzyme with coordinated polymer was practicable.
Key words: chitosan, coordinated polymer, polyphenol oxidases(PPO), immobilization.
CLC Number:
Q554+.9
LEI Fei-hou . STUDY ON IMMOBILIZATION OF POLYPHENOL OXIDASES ON COORDINATED CHITOSAN Cu2+[J]. , 2000, 20(2): 23-26.
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