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Chemistry and Industry of Forest Products ›› 2016, Vol. 36 ›› Issue (3): 53-59.doi: 10.3969/j.issn.0253-2417.2016.03.008

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Callus Culture of Acacia mearnsii De Wilde and Analysis on Proanthocyanidin

XU Ling-ling, YANG Yang, WANG Fei   

  1. College of Chemical Engineering, Nanjing Forestry University, Jiangsu Key Lab. of Biomass-based Green Fuels and Chemicals, Nanjing 210037, China
  • Received:2015-03-23 Online:2016-06-25 Published:2016-07-07

Abstract: This paper studied proanthocyanidins in callus of Acacia mearnsii De Wilde. Callus were obtained by inoculating leaf explants on MS medium, and supplemented with the combination of 6-BA and 2,4-D. The content of total phenolic compounds and proanthocyanidins in the 12 different treatments were tested by Folin-Ciocalteu assay and vanillin assay, respectively. Proanthocyanidins in callus were characterized by ESI-MS. The results showed that the leaf explants of A.mearnsii treated with 0.25 mg/L 6-BA in combination with 2.0 mg/L 2,4-D resulted in a higher rate of callus formation, better growth and maximum content of total phenolic compounds 20.19 mg/g and proanthocyanidins 11.10 mg/g, which is suitable for proliferation culture. We detected monomer, dimer and trace trimer in callus of A.mearnsii, too.

Key words: Acacia mearnsii De Wilde, callus, proanthocyanidin, ESI-MS2

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