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Chemistry and Industry of Forest Products ›› 2017, Vol. 37 ›› Issue (4): 103-109.doi: 10.3969/j.issn.0253-2417.2017.04.015

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Extraction and Performance of Ginkgo biloba Linn. Amylase and Amylopectin

ZHANG Caihong1,2, HUANG Lixin1, XIE Pujun1, DENG Yejun1, CHEN Hongxia1   

  1. 1. Institute of Chemical Industry of Forest Products, CAF;National Engineering Lab. for Biomass Chemical Utilization;Key and Open Lab. of Forest Chemical Engineering, SFA;Key Lab. of Biomass Energy and Material, Jiangsu Province, Nanjing 210042, China;
    2. Research Institute of Forestry New Technology, CAF, Beijing 100091, China
  • Received:2016-10-13 Online:2017-08-25 Published:2017-09-02

Abstract: Starch was extracted and separated from Ginkgo biloba Linn. Its amylase and amylopectin were purified and their performances, e.g. maximum absorption wavelength, DSC, SEM, were analyzed. The results indicated that maximum absorption wavelength on starch, amylase and amylopectin of G. biloba was 600, 620, and 560 nm separately by wavelength scanning from 400 nm to 800 nm. Their particles were less than 20 μm. The SEM images showed that the starch feature was ellipsoid and the amylase and amylopectin particles were not steady feature. The maximum endothermic peak of amylase and amylopectin with 50% water was 138.23℃ and 76.6℃.They were gelatinized. The maximum endothermic peak of amylase and amylopectin with less than 5% water were 117.56℃ and 94.83℃.They were dehydration peaks. The gel permeation chromatographic analysis(GPC) showed that the molecular weights of two amylases were 10.31 ku and 2.32 ku and the molecular weight of amylopectin was 1 189.05 ku.

Key words: Ginkgo biloba Linn., amylase, amylopectin, SEM, DSC

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