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Chemistry and Industry of Forest Products ›› 2018, Vol. 38 ›› Issue (4): 103-108.doi: 10.3969/j.issn.0253-2417.2018.04.016

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Simultaneous Determination of Isobiflorin and Biflorin in Fujian Euscaphis japonica by HPLC

NI Lin1,2, QIU Yatie1, LI Yanlei2,3, ZOU Xiaoxing2,3, XU Huiyou1   

  1. 1. College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
    2. Fujian Colleges and Universities Engineering Research Institute of Conservation & Utilization of Natural Bioresources, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
    3. College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China
  • Received:2018-01-09 Online:2018-08-25 Published:2018-08-10

Abstract: The method for simultaneous determination of isobiflorin and biflorin in Euscaphis japonica(Thunb.) Dippel was established by HPLC, and their contents in twigs, leaves, epicarp, and seeds of E. japonica from 10 different producing areas in Fujian province were determined. The determination was carried out on Dikma Diamonsil C18 column (250 mm×4.6 mm, 5 μm) with anisocraticmobile phaseof 25% methanol.The flow rate was 1.0 mL/min, the UV detection wavelength was set at 256 nm, the column temperature was 30℃, and the detection time was 15 min. The isobiflorin(A) and biflorin(B) showed good linearity in the range of 2.000 2-20.002 0 μg (r=0.999 9) and 2.001 6-20.016 0 μg (r=0.999 9), respectively. The average recoveries of isobiflorin and biflorin were 98.42% and 99.77% with the RSD of 0.88% and 0.96%, respectively. The developed method was found to be rapid, stable and accurate, and could be used to control the quality of E. japonica development and application of traditional Chinese medicine.

Key words: Euscaphis, chromone-C-glucosides, HPLC

CLC Number: