银杏叶聚戊烯醇的分离纯化及其亲水衍生物的合成

doi:10.3969/j.issn.0253-2417.2019.01.006

Abstract

Abstract:

The analytical methodology and method performance for the determination of polyprenol in Ginkgo biloba leaves were investigated for the first time. The results showed that of the high performance liquid chromatography with C18 as chromatographic column, isopropanol methanol(volume ratio, 32:18) as mobile phase, 210 nm as detection wavelength showed high precision(RSD=0.89%) and good stability(RSD=3.26%). In addition, the average standard recovery and RSD value of polyprenol were 97.4% and 1.75%, respectively. Then, polyprenol with the purity of 98.6% was obtained by extraction with n-hexane, saponification, de-waxing with ethanol and acetone, and silica gel column chromatography(V(diethyl ether):V(n-hexane)=3:97). On this basis, the terminal hydroxyl group of high-purity polyprenol was modified to synthesize aminopolyprenol. Firstly, by mitsunobu reaction with phthalimide, followed by reduction reaction with hydrazine hydrate, to obtain aminopolyprenol. The structure of amino polyprenol was characterized by infrared and nuclear magnetic resonance spectroscopy, which confirmed the aminopolyprenol was successfully synthesized.

Key words: polyprenol, separation, purification, hydrophilic modification

CLC Number:

TQ35

Changwei ZHANG,Yulu MA,Ran TAO,Jianzhong YE,Chengzhang WANG. Separation, Purification and Hydrophilic Derivative Synthesis of Ginkgo biloba Leaves Polyprenol[J]. Chemistry and Industry of Forest Products, 2019, 39(1): 41-45.

Figures/Tables 3

References 16

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样品/mg sample	加入量/mg addition amount	理论值/mg theoretical value	测得总量/mg total amount	回收率/% recovery rate	平均回收率/% average recovery rate	RSD/%
0.366	0.200	0.566	0.585	103.4	102.7	1.79
0.366	0.200	0.566	0.576	101.8
0.366	0.300	0.666	0.672	100.9
0.366	0.300	0.666	0.671	100.8
0.366	0.400	0.766	0.799	104.3
0.366	0.400	0.766	0.804	105.0

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Separation, Purification and Hydrophilic Derivative Synthesis of Ginkgo biloba Leaves Polyprenol

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