蒸汽爆破预处理对杜仲皮活性成分和杜仲胶提取的影响

doi:10.3969/j.issn.0253-2417.2019.01.013

Abstract

Abstract:

Eucommia ulmoides Oliver bark was pretreated by steam explosion, and then the pretreated bark was hydrolyzed by cellulase to extract bioactive components and gutta-percha. Effect of steam explosion pretreatment on the bioactive components, yield and molecular weight of gutta-percha from bark of E. ulmoides Oliver were investigated. The results showed that the optimal conditions for the extraction of bioactive components from untreated barks using ethonal sonication-assisted method were 40% ethanol, ultrasonic power 200 W, extraction temperature 40 ℃ and extraction time 40 min. When the conditions of steam explosion pretreatment were 3.25 MPa and 1 min, the yields of flavonoids and chlorogenic acid using ethonal sonication-assisted method were 26.83 and 1.22 mg/g, respectively, and the yields of flavonoids and chlorogenic acid extracted by cellulase hydrolysis were 19.17 and 0.93 mg/g, respectively. The solid residue of pretreated barks after enzymatic hydrolysis was used to extract gutta-percha. When the conditions of steam explosion pretreatment were 0.52 MPa and 15 min, the yields of gutta-percha was 5.92%, and the M_w and M_n of gutta-percha were 28.39×10⁴ and 6.45×10⁴, respectively.

Key words: Eucommia ulmoides Oliver bark, steam explosion, bioactive components, gutta-percha, enzymatic hydrolysis

CLC Number:

TQ35

Jinjin WEI,Donglin XIN,Xiang CHEN,Xiyu QUAN,Junhua ZHANG. Effect of Steam Explosion on Extraction of Bioactive Components and Gutta-percha from Barks of Eucommia ulmoides Oliver[J]. Chemistry and Industry of Forest Products, 2019, 39(1): 88-94.

Figures/Tables 5

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Table 5

References 18

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提取溶剂extraction solvent	黄酮/(mg·g^-1)flavonoids	桃叶珊瑚苷/(mg·g^-1)aucubin	京尼平苷酸/(mg·g^-1)geniposidic acid	绿原酸/(mg·g^-1)chlorogenic acid
缓冲液buffer	5.93±0.00	0.98±0.15	0.54±0.01	0.24±0.05
纤维素酶cellulase	7.52±0.16	1.13±0.10	0.35±0.01	0.11±0.03
水water	5.08±0.07	0.70±0.34	0.45±0.08	0.10±0.04
30%乙醇30% ethanol	6.53±0.14	1.81±0.00	0.63±0.04	0.34±0.02
60%乙醇60% ethanol	6.07±0.05	1.66±0.05	0.72±0.01	0.24±0.08

提取条件 extraction condition		黄酮/(mg·g^-1) flavonoids	桃叶珊瑚苷/(mg·g^-1 aucubin	京尼平苷酸/(mg·g^-1) geniposidic acid	绿原酸/(mg·g^-1) chlorogenic acid
乙醇体积分数volume fraction of ethanol	20%	5.74±0.23	7.74±0.44	0.54±0.01	0.20±0.01
	30%	6.29±0.02	9.26±0.65	0.69±0.05	0.21±0.00
	40%	7.01±0.11	11.55±0.65	0.62±0.03	0.24±0.01
	50%	6.50±0.23	8.23±0.71	0.75±0.08	0.26±0.04
	60%	6.31±0.50	8.45±0.16	0.71±0.01	0.27±0.05
	70%	7.15±0.21	25.53±0.92	0.78±0.02	0.26±0.00
提取时间extraction time	20 min	5.59 ±0.02	7.36±0.60	0.67±0.06	0.23±0.01
	40 min	5.70±0.13	7.74±0.22	0.59±0.02	0.28±0.03
	60 min	3.47±0.07	8.45±0.60	0.58±0.01	0.29±0.00
	80 min	4.27±0.29	9.64±0.71	0.59±0.00	0.25±0.01
	100 min	3.89±0.35	8.72±0.87	0.58±0.10	0.28±0.02
	120 min	3.65±0.11	8.12±0.27	0.54±0.03	0.22±0.00
超声波功率ultrasonic power	100 W	6.33±0.48	9.15±0.54	0.64±0.04	0.25±0.01
	200 W	6.77±0.13	9.53±0.27	0.59±0.02	0.28±0.04
	300 W	5.85±0.13	9.86±0.27	0.58±0.00	0.19±0.00
	400 W	6.37±0.09	10.02±0.22	0.64±0.01	0.24±0.00
	500 W	6.02±0.13	9.53±0.49	0.59±0.03	0.21±0.01
提取温度extraction temperature	25 ℃	7.66±0.05	6.22±0.65	0.61±0.02	0.29±0.06
	40 ℃	7.63±0.08	7.30±1.09	0.69±0.06	0.25±0.01
	55 ℃	9.11±0.45	7.79±0.82	0.64±0.03	0.29±0.00
	65 ℃	9.34±0.28	7.85±0.65	0.65±0.01	0.29±0.04

预处理pretreatment		黄酮/(mg·g^-1)flavonoids	桃叶珊瑚苷/(mg·g^-1)aucubin	京尼平苷酸/(mg·g^-1)geniposidic acid	绿原酸/(mg·g^-1)chlorogenic acid
压力/MPapressure	时间/mintime	黄酮/(mg·g^-1)flavonoids	桃叶珊瑚苷/(mg·g^-1)aucubin	京尼平苷酸/(mg·g^-1)geniposidic acid	绿原酸/(mg·g^-1)chlorogenic acid
0	0	7.63±0.08	7.30±1.09	0.69±0.06	0.25±0.01
0.52	8	5.08±0.46	1.23±0.41	0.44±0.11	0.23±0.01
0.52	15	3.85±0.33	0.93±0.49	0.33±0.02	0.32±0.01
0.90	6	8.30±1.75	1.15±0.16	0.24±0.04	0.21±0.05
1.45	3	14.04±1.23	1.83±0.08	0.36±0.12	0.41±0.03
1.45	8	17.68±0.83	2.18±0.49	0.49±0.06	0.73±0.01
2.10	6	21.82±0.42	2.16±0.14	0.29±0.08	1.01±0.06
3.25	1	26.83±0.04	2.46±0.05	0.33±0.03	1.22±0.02

预处理 pretreatment		活性成分/(mg·g^-1) active compounds				糖得率/(mg·g^-1) yield of saccharide
压力/ MPapressure	时间/ mintime	黄酮 flavonoids	桃叶珊瑚苷 aucubin	京尼平苷酸 geniposidic acid	绿原酸 chlorogenic acid	葡萄糖glucose	木糖xylose
0	0	10.12±0.04	1.78±0.03	0.00±0.00	0.37±0.01	97.86±0.72	22.75±0.08
0.52	8	10.41±0.07	0.99±0.04	0.13±0.01	0.67±0.00	111.11±4.11	21.81±0.24
0.52	15	8.44±0.13	0.80±0.06	0.11±0.02	0.60±0.02	103.46±3.02	27.00±0.14
0.90	6	9.98±0.04	0.55±0.01	0.02±0.01	0.50±0.00	110.56±4.15	31.21±0.92
1.45	3	12.36±0.20	0.68±0.03	0.03±0.00	0.56±0.02	124.89±19.46	35.63±3.88
1.45	8	13.86±0.10	0.63±0.05	0.00±0.00	0.24±0.24	102.76±2.47	30.34±0.06
2.10	6	16.86±0.22	0.50±0.03	0.07±0.00	0.88±0.00	104.57±2.16	35.61±0.07
3.25	1	19.17±0.25	0.53±0.02	0.09±0.01	0.93±0.02	100.45±2.93	36.41±1.52

压力/MPapressure	时间/mintime	Mw	M_n	D=M_w/M_n	杜仲胶得率/%yield of gutta-percha
0	0	42.70×10⁴	16.36×10⁴	2.61	4.82
0.52	8	29.36×10⁴	7.58×10⁴	3.87	5.36
0.52	15	28.39×10⁴	6.45×10⁴	4.40	5.92
0.90	6	26.64×10⁴	6.76×10⁴	3.94	4.73
1.45	3	24.48×10⁴	5.74×10⁴	4.26	4.36
1.45	8	29.64×10⁴	7.21×10⁴	4.11	4.97
2.10	6	33.58×10⁴	9.92×10⁴	3.39	4.35
3.25	1	32.35×10⁴	8.47×10⁴	3.82	4.61

Effect of Steam Explosion on Extraction of Bioactive Components and Gutta-percha from Barks of Eucommia ulmoides Oliver

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