绵马贯众多酚的分离纯化及其抗氧化活性研究

doi:10.3969/j.issn.0253-2417.2019.02.012

Abstract

Abstract:

The polyphenols were extracted from Cyrtomium fortune with 70% ethanol, and then purified with 7 macroporous resins. The results showed that HPD100 resin had the best purification effect based on the adsorption rate and desorption rate. The effect of sample mass concentration, loading speed of sample, volume fraction of desorption ethanol, desorption speed and sample pH value on the purification of HPD100 resin was further analyzed, and the optimum purification conditions were obtained as follows: mass concentration of sample solution 2.00 g/L, pH value 5, loading speed of sample 1 mL/min, volume fraction of desorption ethanol 70% and desorption speed 2 mL/min. Under these conditions, the content of polyphenols increased from 27.64% to 54.00% (the yield of polyphenols was 6.46%). The purified polyphenols showed higher antioxidant activity. For the purified polyphenols, the IC₅₀of DPPH radical (DPPH·) and hydroxyl radical (·OH) were 0.01 and 0.49 g/L, respectively; but for the crude polyphenols, the IC₅₀ of DPPH· and ·OH were 0.05 and 1.79 g/L, respectively. At 0.04 g/L, the DPPH· scavenging activity of the purified polyphenols was 90.82%, almost similar to that of Vc. At 0.05 g/L, ferric ion reducing power of the purified polyphenols was 0.47, but the crude polyphenols was only 0.25. Furthermore, the purified polyphenols exhibited better DPPH· scavenging activity and ferric ion reduction ability under acidic conditions and the hydroxyl radical scavenging activity under alkaline condition.

Key words: Cyrtomium fortune, polyphenols, purification with macroporous resin, antioxidant activity

CLC Number:

TQ35

Huifang ZHANG,Ye WANG,Weichao HU,Shu YUAN,Ming YUAN. Purification and Antioxidation Activity of Polyphenol from Cyrtomium fortune[J]. Chemistry and Industry of Forest Products, 2019, 39(2): 81-88.

Figures/Tables 11

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树脂 resin	极性 polarity	粒径/mm particle size	孔径/nm pore size	比表面积/(m²·g^-1) specific surface area
S-8	强极性strong polarity	0.30~1.25	28~30	100~120
X-5	非极性nonpolar	0.32~1.25	290~300	500~600
DM301	中极性moderate polarity	0.30~1.25	13~17	330~380
D101	非极性nonpolar	0.30~1.25	9~10	500~550
CAD-40	中极性moderate polarity	0.30~1.25	7~8	450~500
AB-8	非极性nonpolar	0.30~1.25	130~140	450~520
HPD100	非极性nonpolar	0.30~1.25	8.50~9.00	650~700

树脂 resin	吸附量/(mg·g^-1) adsorption capacity	吸附率/% adsorption ratio	解吸量/(mg·g^-1) desorption capacity	解吸率/% desorption ratio
S-8	18.86±0.09	91.06	2.31±0.05	12.27
X-5	18.83±0.10	90.90	16.33±0.69	86.71
DM301	18.23±0.32	88.12	13.53±0.53	74.13
D101	18.85±0.08	91.03	17.45±0.41	92.56
CAD-40	18.68±0.26	90.20	16.68±0.83	89.28
AB-8	18.55±0.23	89.55	17.14±0.64	92.42
HPD100	18.72±0.38	90.35	17.92±1.20	95.76

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Purification and Antioxidation Activity of Polyphenol from Cyrtomium fortune

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