油橄榄叶提取物的制备及理化性质研究

doi:10.3969/j.issn.0253-2417.2019.04.012

Abstract

Abstract:

The scale-up experiment of hot reflux extraction of total polyphenols, total flavonoids and oleuropein from olive leaves was studied. The effect of ethanol concentration on the extraction of active substances was investigated, and the related kinetics was also evaluated by ExpDec1 model. The results showed the highest contents of total polyphenols, total flavonoids and oleuropein were obtained when the ethanol concentration was 80% and they were 3.77, 2.85 and 3.03 g/L, respectively. ExpDec1 model had a good fitting effect, and the fitting degree R value was higher than 0.9. The higher ethanol concentration, the better the fitness effect observed. Under the condition of inlet temperature 150℃ and feeding rate 15 mL/min, the extracts were dried by spray drying, and crude extracts were purified by AB-8 macroporous resin, the content of total polyphenols increased from 9.71% to 31.65%, the content of total flavonoids increased from 7.22% to 10.98%, and the content of oleuropein increased from 9.16% to 20.38%. The physicochemical properties of the extract powder were measured, such as moisture content, porosity, density, dissolution, moisture absorption, active substance, etc. The results showed that moisture content, density and porosity of different extracts were similar, water extract had better water solubility(98.38%), 80% ethanol extract had higher content of the active substance(the contents of total polyphenols, total flavonoids and oleuropein were 9.71%, 7.22% and 9.16%, respectively), and the contents of total flavonoids and oleuropein were about two times higher than that of other solvents.

Key words: olive leaves, extraction, macroporous resin, total polyphenols, total flavonoids, oleuropein

CLC Number:

TQ35
TS201.1

Xiaojie WANG,Lixin HUANG,Caihong ZHANG,Pujun XIE,Yejun DENG,Lujie LIU. Preparation and Physicochemical Properties of Olive Leaf Extract[J]. Chemistry and Industry of Forest Products, 2019, 39(4): 83-90.

Figures/Tables 6

Fig.1

Table 1

Table 2

Table 3

Fig.2

Table 4

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活性物质 active compounds	提取溶剂 extraction solvents	模型拟合参数fitting parameters of models			R²
活性物质 active compounds	提取溶剂 extraction solvents	y₀	A₁	k₁	R²
总多酚 total polyphenols	H₂O	2.709 42	-1.883 62	1.594 11	0.988 40
	20% EtOH	2.635 21	-3.149 17	0.973 13	0.996 20
	50% EtOH	3.004 61	-4.966 44	0.818 93	0.984 22
	80% EtOH	3.670 18	-5.605 37	1.373 00	0.995 68
总黄酮 total flavonoids	H₂O	0.623 77	-0.487 55	1.283 85	0.930 58
	20% EtOH	0.903 74	-0.394 43	1.442 63	0.983 00
	50% EtOH	1.730 31	-0.892 99	1.287 22	0.981 09
	80% EtOH	2.902 72	-5.808 09	1.175 29	0.997 74
橄榄苦苷 oleuropein	H₂O	1.434 81	-0.769 68	2.249 48	0.984 40
	20% EtOH	1.885 04	-2.209 87	1.233 17	0.992 24
	50% EtOH	1.824 57	-0.409 69	1.358 00	0.998 91
	80% EtOH	2.997 99	-6.435 99	1.029 46	0.999 30

溶剂 solvent	时间/h time	总多酚total polyphenols			总黄酮total flavonoids			橄榄苦苷oleuropein
溶剂 solvent	时间/h time	W_P	W_M	R	W_P	W_M	R	W_P	W_M	R
H₂O	2.5	2.21	2.11	0.95	0.55	0.55	0.99	1.18	1.16	0.99
H₂O	7.5	2.67	2.41	0.90	0.62	0.61	0.98	1.41	1.38	0.98
20% EtOH	2.5	2.39	2.20	0.92	0.83	0.77	0.93	1.59	1.54	0.97
20% EtOH	7.5	2.63	2.57	0.98	0.90	0.89	0.99	1.88	1.79	0.95
50% EtOH	2.5	2.77	3.0	0.91	1.60	1.76	0.91	1.76	1.68	0.95
50% EtOH	7.5	3.00	3.31	0.91	1.73	1.91	0.91	1.82	1.88	0.97
80% EtOH	2.5	2.76	3.02	0.92	2.21	2.30	0.96	2.43	2.49	0.98
80% EtOH	7.5	3.65	3.58	0.98	2.89	2.86	0.99	2.99	3.13	0.96

%
提取溶剂 extraction solvent	总多酚total polyphenols		总黄酮total flavonoids		橄榄苦苷oleuropein
提取溶剂 extraction solvent	质量分数 mass fraction	损失率 loss rate	质量分数 mass fraction	损失率 loss rate	质量分数 mass fraction	损失率 loss rate
H₂O	8.04±0.34	52	2.80±0.01	31	3.93±0.27	54
20% EtOH	8.56±0.24	56	3.26±0.05	46	4.26±0.24	54
50% EtOH	9.37±0.38	48	4.40±0.09	54	5.35±0.29	54
80% EtOH	9.71±0.05	55	7.22±0.02	55	9.16±0.21	53

提取溶剂¹⁾ extraction solvent	含水量/% moisture content	真密度/(g·mL^-1) true density	体积密度/(g·mL^-1) bulk density	孔隙率 porosity	吸湿量/% hygroscopicity	水溶性/% water solubility
H₂O	1.01	1.256	0.476	0.621	8.09	98.38
20% EtOH	0.74	1.205	0.445	0.631	3.21	88.58
50% EtOH	0.68	1.324	0.465	0.648	2.60	88.38
80% EtOH	0.86	1.343	0.489	0.636	3.07	64.18
80%EtoH^*	0.75	1.356	0.476	0.648	2.91	77.40

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