五味子果肉和种子精油的化学成分与生物活性对比研究

doi:10.3969/j.issn.0253-2417.2019.06.008

Abstract

Abstract:

In order to investigate the differences and similarities between the essential oils (EOs) obtained from Schisandra chinensis fruits pulp and seeds (PEO and SEO), the chemical composition, anti-tumor property, antimicrobial and antioxidant activity were examined. According to the results of GC-MS (gas chromatography-mass spectrometry, GC-MS) analyses, 55 and 45 compounds which represented 94.04% and 93.39% of the oils were identified, respectively. In addition, anti-tumor activity of essential oils was carried out using the Sulforhodamine B (SRB) method. The oils also revealed the highest anti-tumor activity against HeLa and BGC-823 cells, but they had no effect on A549 cells. They presented IC₅₀ values from 39.07 mg/L to 56.43 mg/L. PEO and SEO also exhibited inhibitory activity against four bacterial strains including Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa and Escherichia coli. MIC of PEO were 245.00-504.33 mg/L, MIC of SEO were 495.00-1 004.68 mg/L. Furthermore, in all assays of antioxidant ability, PEO showed stronger antioxidant properties than SEO. IC₅₀ value of PEO against DPPH and ABTS radical were 3.15 and 2.31 g/L, respectively. IC₅₀ of SEO against DPPH and ABTS radical were 5.43 and 10.11 g/L, respectively. In a word, this study supports the potentials of PEO and SEO as antibacterial and antineoplastic agent.

Key words: Schisandra chinensis, essential oils, anti-tumor activity, antibacterial activity, antioxidant activity

CLC Number:

TQ35

Jie WANG,Jiaojiao LU,Han DU,Meng ZHANG,Panpan LIU,Xiaoqiang CHEN. Comparative Study on Essential Oils of Schisandra chinensis Fruits Pulp and Seeds: Chemical Composition and Bioactivities[J]. Chemistry and Industry of Forest Products, 2019, 39(6): 59-67.

Figures/Tables 5

No.	compound	retention time/min	percentage/%		RI_exp	RI_liter
No.	compound	retention time/min	PEO	SEO	RI_exp	RI_liter
1	α-thujene	5.831	0.80	1.32	925	924
2	α-pinene	5.976	6.95	2.35	938	939
3	camphene	6.041	—	2.47	957	957
4	sabinene	6.418	1.01	0.72	975	973
5	myrcene	6.607	1.74	0.52	991	993
6	α-phellandrene	6.792	0.88	0.31	1005	1006
7	α-terpinene	6.849	0.75	0.66	1010	1012
8	p-cymene	6.955	2.76	1.38	1019	1021
9	β-ocimene	7.214	8.76	8.15	1038	1038
10	γ-terpinene	7.517	2.02	1.54	1061	1057
11	trans-sabinene hydrate	7.936	1.88	0.88	1093	1093
12	α-thujone	8.119	2.04	—	1107	1105
13	terpinen-4-ol	9.208	0.30	0.20	1183	1180
14	myrtenol	9.408	0.37	—	1198	1196
15	cuminaldehyde	9.721	0.22	—	1219	1218
16	thymol methyl ether	10.058	4.82	4.84	1241	1239
17	thymol	10.832	2.41	0.92	1292	1293
18	copaene	11.572	1.29	0.02	1342	1340
19	α-cubebene	11.710	2.69	0.02	1350	1348
20	α-ylangene	12.129	4.71	23.28	1376	1373
21	methyleugenol	12.454	0.28	—	1402	1403
22	α-gurjunene	12.607	1.00	0.60	1410	1408
23	caryophyllene	12.666	0.63	0.91	1416	1415
24	β-caryophyllene	12.728	1.13	0.38	1421	1420
25	γ-elemene	12.900	1.56	1.68	1432	1432
26	farnesene	12.965	0.76	—	1437	1438
27	aromadendrene	13.048	2.21	2.47	1442	1442
28	α-humulene	13.228	—	0.76	1455	1455
29	allo-aromadendrene	13.310	0.64	—	1460	1460
30	γ-selinene	13.485	0.98	2.59	1472	1472
31	cis-eudesma-6, 11-diene	13.549	1.11	2.85	1476	1476
32	bicyclogermacrene	13.721	2.52	1.17	1490	1492
33	α-muurolene	13.933	—	1.16	1503	1503
34	calamenene	14.199	1.94	—	1521	1520
35	δ-cadinene	14.266	—	1.54	1526	1526
36	α-cadinene	14.350	0.14	5.89	1532	1532
37	trans-cadina-1, 4-diene	14.389	0.48	—	1535	1535
38	α-calacorene	14.493	1.57	2.67	1542	1541
39	germacrene B	14.508	—	3.05	1543	1544
40	cis-α-copaen-8-ol	14.687	0.94	2.26	1555	1553
41	trans-nerolidol	14.737	0.59	0.30	1559	1561
42	ledol	14.838	2.79	2.04	1566	1565
43	geranyl butanoate	14.922	0.35	1.41	1572	1571
44	spathulenol	14.988	0.66	—	1576	1577
45	epiglobulol	15.249	—	1.09	1595	1594
46	humulene epoxide Ⅱ	15.427	3.12	0.75	1607	1608
47	caryophylladienol Ⅱ	15.604	0.56	—	1620	1621
48	γ-eudesmol	15.757	0.52	—	1631	1628
49	t-muurolol	15.925	0.57	0.37	1643	1644
50	torreyol	15.972	1.12	—	1646	1645
51	α-cadinol	16.107	0.49	—	1656	1654
52	selin-11-en-4β-ol	16.176	0.46	0.10	1661	1662
53	epi-globulol	16.295	3.02	1.32	1670	1670
54	α-bisabolol	16.435	1.19	—	1680	1684
55	α-cyperone	16.724	1.66	0.81	1700	1704
56	dihydrocostunolide	17.179	1.48	—	1734	1735
57	β-(Z)-curcumen-12-ol	17.318	1.90	—	1745	1751
58	diepi-α-cedrene epoxide	17.682	2.23	1.75	1772	1768
59	2, 2, 6-trimethyl-1-(3-oxo-but-1-enyl)-7-oxa-bicyclo-[4.1.0]-hept-4-en-3-one	17.882	1.46	1.25	1787	1783
60	1, 4-dimethyl-7-(1-methylethyl)-azulene	18.348	2.76	2.26	1822	1825
61	isolongifolen-9-one	18.602	0.82	0.76	1842	1837
	monoterpenes hydrocarbons		25.67	19.43
	oxygenated monoterpene		4.81	1.08
	sesquiterpenes hydrocarbons		28.12	53.31
	oxygenated sesquiterpenes		27.58	12.79
	others		7.86	6.77
	total		94.04	93.39

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sample	HeLa cells	A549 cells	BGC-823 cells
PEO	56.43±0.81	>100	39.07±1.13
SEO	53.53±1.42	>100	50.11±1.75
Taxol	0.38±0.02	0.02±0.01	0.012±0.01

microbial strains	PEO		SEO
microbial strains	MIC	MBC	MIC	MBC
S. aureus	245.00±14.55	496.67±13.82	503.55±10.00	999.33±33.21
B. subtilis	253.33±10.58	1001.22±52.18	495.00±22.41	2002.32±60.89
E. coli	504.33±43.88	998.67±33.32	1004.68±25.65	1997.67±72.35
P. aeruginosa	250.24±17.46	995.89±42.18	501.00±12.89	1002.91±44.64

sample	DPPH assay	ABTS assay
PEO	3.15±0.11	2.31±0.07
SEO	5.43±0.17	10.11±0.27

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Comparative Study on Essential Oils of Schisandra chinensis Fruits Pulp and Seeds: Chemical Composition and Bioactivities

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