生长条件对肉桂叶精油含量、组成及抑菌活性的影响

doi:10.3969/j.issn.0253-2417.2020.02.006

Abstract

Abstract:

Essential oils of cinnamon leaves from different growth conditions were extracted by steam distillation method, respectively. The chemical constituents were analyzed by gas chromatography-mass spectrometry, and the relative content of each constituent was determined by area normalization. Their antimicrobial activities on three common pathogens were tested by filter paper method. The results indicated that the essential oil yield of cinnamon leaves were affected by tree age, planting density and slope aspect. Among them, tree age had the greatest impact. The highest yield of essential oil was obtained from 15-year-old sparse planting cinnamon leaves (No.3, 1.60%), the lowest was obtained from 20-year-old (No.7, 0.51%). The second was slope aspect, yield of essential oil obtained from 15-year-old south slope cinnamon leaves (No.5, 1.20%) was much higher than that obtained from 15-year-old north slope cinnamon leaves (No.6, 0.55%). The minimal impact was caused by planting density, yield of essential oil obtained form 15-year-old sparse planting cinnamon leaves (No.3, 1.60%) was higher than that obtained from 15-year-old close planting cinnamon leaves (No.4, 1.34%). The components and their GC contents of essential oil of cinnamon leaves from different growth conditions were different. A total of 28 chemical constituents were identified in the seven samples, among them, 15-year-old sparse planting (No.3) had the most kinds of essential oil components (21 kinds), 15-year-old dense plants (No.4) and 15-year-old south slope (No.5) contain the least kinds (both of 7 kinds). Trans-cinnamaldehyde was the most abundant component in the samples, of which the highest GC content was in No.4 (90.26%), and the lowest GC content was in 10-year-old (No.2, 67.73%). The common components were benzaldehyde, benzenepropanal, cis-cinnamaldehyde and trans-cinnamaldehyde. The order of inhibition effect on three experimental bacteria was Staphylococcus aureus > Escherichia coli > Pseudoinonal aeruginosa at the mass concentrations of 500 g/L. The diameters ranges of bacteriostasis circles were 22.57-39.63 mm, 27.58-34.31 mm and 16.39-23.89 mm, respectively. Among them, 20-year-old (No.7) had the strongest inhibition on S. aureus(39.63 mm), 15-year-old close planting (No.4) had the strongest inhibition on E. coli(34.31 mm) and 5-year-old (No.1) had the strongest inhibition on P. aeruginosa(23.89 mm).

Key words: cinnamon leaves, cinnamaldehyde, tree age, slope aspect, planting density, antimicrobial activity

CLC Number:

TQ35
R284

Zuohui ZHANG,Yongqing TONG,Guangzhi HUANG,Liangwu BI. Effects of Growth Conditions on Content, Components and Antimicrobial Activity of Volatile Oil of Cinnamon Leaves[J]. Chemistry and Industry of Forest Products, 2020, 40(2): 49-54.

Figures/Tables 4

Table 1

Fig.1

Table 2

Volatile compounds identified in the cinnamon leaves from different growth conditions"

序号 No.	t_R/min	化合物 compound	分子式 fomula	GC含量GC contents /%
序号 No.	t_R/min	化合物 compound	分子式 fomula	No.1	No.2	No.3	No.4	No.5	No.6	No.7
1	3.719	水芹烯phellandrene	C₁₀H₁₆	—	—	—	0.07	0.12	0.15	—
2	3.720	α-蒎烯α-pinene	C₁₀H₁₆	—	—	0.23	—	—	—	—
3	3.954	莰烯camphene	C₁₀H₁₆	—	—	0.15	0.02	—	—	—
4	4.109	苯甲醛benzaldehyde	C₇H₆O	3.02	2.74	1.35	2.49	3.97	1.91	2.17
5	4.322	β-蒎烯β-pinene	C₁₀H₁₆	—	—	0.16	—	—	—	—
6	5.057	D-柠檬烯D-limonene	C₁₀H₁₆	—	—	0.06	—	—	—	—
7	5.399	邻羟基苯甲醛o-hydroxybenzaldehyde	C₇H₆O₂	1.41	1.34	0.09	—	—	0.14	2.19
8	6.671	苯乙醇phenethyl alcohol	C₈H₁₀O	—	—	—	—	—	—	0.77
9	7.584	苯丙醛benzenepropanal	C₉H₁₀O	1.65	3.43	0.88	1.67	2.97	1.16	1.05
10	8.835	顺式肉桂醛cis-cinnamaldehyde	C₉H₈O	0.87	0.86	0.78	0.77	1.03	0.88	0.79
11	9.281	邻甲氧基苯甲醛o-anisaldehyde	C₈H₈O₂	—	0.89	0.52	—	—	0.48	0.71
12	9.484	乙酸苯乙酯phenethyl acetate	C₁₀H₁₂O₂	0.19	2.00	0.14	—	0.14	0.19	0.08
13	10.082	反式肉桂醛trans-cinnamaldehyde	C₉H₈O	76.09	67.73	77.11	90.26	83.42	88.17	77.45
14	12.086	邻甲氧基苯丙酮o-methoxyphenylacetone	C₁₀H₁₂O₂	—	—	0.49	—	—	—	0.20
15	12.298	α-古巴烯α-copaene	C₁₅H₂₄	2.47	1.25	—	—	—	—	0.87
16	12.312	α-荜澄茄油烯α-cubebene	C₁₅H₂₄	—	—	0.05	—	—	—	—
17	13.332	石竹烯caryophyllene	C₁₅H₂₄	—	—	0.06	—	—	—	—
18	13.759	乙酸肉桂酯cinnamyl acetate	C₁₀H₁₂O₂	3.46	5.74	0.50	—	0.83	—	—
19	13.813	香豆素coumarin	C₉H₆O₂	—	—	—	—	—	—	3.87
20	14.477	(1α, 4aα, 8aα)-1, 2, 3, 4, 4a, 5, 6, 8a-八氢-7-甲基 -4-亚甲基-1-(1-甲基乙基)-萘 (1α, 4aα, 8aα )-1, 2, 3, 4, 4a, 5, 6, 8a-octahydro-7-methyl -4-methylene-1-(1-methylethyl) -naphthalene	C₁₅H₂₄	—	—	0.05	—	—	—	—
21	14.970	α-依兰油烯α-muurolene	C₁₅H₂₄	—	—	—	—	—	—	0.09
22	15.090	β-没药烯β-bisabolene	C₁₅H₂₄	—	—	—	—	—	—	0.09
23	15.381	β-杜松烯β-cadinene	C₁₅H₂₄	0.31	—	0.05	—	—	—	0.23
24	15.653	邻甲氧基肉桂醛o-methoxycinnamaldehyde	C₁₀H₁₀O₂	—	2.84	13.31	0.65	—	2.91	4.18
25	16.204	反式-橙花叔醇trans-nerolidol	C₁₅H₂₆O	—	0.55	0.43	—	—	—	0.14
26	16.778	桉油烯醇espatulenol	C₁₅H₂₄O	—	—	0.14	—	—	—	0.15
27	16.848	氧化石竹烯caryophyllene oxide	C₁₅H₂₄O	—	—	0.06	—	—	—	0.17
28	20.506	苯甲酸苄酯benzyl benzoate	C₁₄H₁₂O₂	—	—	—	—	—	—	0.14
合计total				89.47	89.37	96.61	95.93	92.48	95.99	95.34

Table 2

Table 3

References 15

1	黎贵卿, 陆顺忠, 江燕, 等. 不同生长阶段肉桂叶中油细胞的形态及精油成分[J]. 广西林业科学, 2016, 45 (1): 85- 88.
	LI G Q , LU S Z , JIANG Y , et al. Morphology of oil cells in Cinnamomum cassia leaves with different growth stages and components of essential oil[J]. Guangxi Forestry Science, 2016, 45 (1): 85- 88.
2	郭虹, 林观样. 肉桂叶挥发性成分分析[J]. 浙江中医药大学学报, 2009, 33 (6): 883- 884.
	GUO H , LIN G X . Cinnamon leaf volatile component analysis[J]. Journal of Zhejiang University of Traditional Chinese Medicine, 2009, 33 (6): 883- 884.
3	WANG R , WANG R J , YANG B . Extraction of essential oils from five cinnamon leaves and identification of their volatile compound compositions[J]. Innovative Food Science & Emerging Technologies, 2009, 10 (2): 289- 292.
4	ĆOSIĆ J , VRANDEČIĆ K , POŠTIĆ J , et al. In vitro antifungal activity of essential oils on growth of phytopathogenic fungi[J]. Agriculture, 2010, 16 (2): 25- 28.
5	CHANG C T , CHANG W L , HSU J C , et al. Chemical composition and tyrosinase inhibitory activity of Cinnamomum cassia essential oil[J]. Botanical Studies, 2013, 54 (10): 1- 7.
6	张浩, 柴向华. 肉桂叶精油水蒸气提取工艺及甄别技术的研究[J]. 广东工业大学学报, 2017, 34 (4): 27- 30, 40.
	ZHANG H , CHAI X H . A research on steam extracting and screening technology of cinnamon leaf essential oil[J]. Journal of Guangdong University of Technology, 2017, 34 (4): 27- 30, 40.
7	DUAN J , ZHAO Y . Antimicrobial efficiency of essential oil and freeze-thaw treatments against Escherichia coli O157:H7 and Salmonella enterica Ser.Enteritidis in strawberry juice[J]. Journal of Food Science, 2009, 74 (3): 131- 137.
8	MELGAREJO-FLORES B G , ORTEGA-RAMÍREZ L A , SILVA-ESPINOZA B A , et al. Antifungal protection and antioxidant enhancement of table grapes treated with emulsions, vapors, and coatings of cinnamon leaf oil[J]. Postharvest Biology and Technology, 2013, 86, 321- 328.
9	张笮晦, 童永清, 黄广智, 等. 肉桂叶化学成分及药理作用研究进展[J]. 广州化工, 2019, 47 (1): 20- 22.
	ZHANG Z H , TONG Y Q , HUANG G Z , et al. Research progress on chemical components and pharmacological activities of Cinnamomum cassia leaves[J]. Guangzhou Chemical Industry, 2019, 47 (1): 20- 22.
10	卫向南, 李伟光, 刘雄民, 等. 肉桂叶水扩散蒸馏提取肉桂油的研究[J]. 应用化工, 2014, 43 (6): 1047- 1049.
	WEI X N , LI W G , LIU X M , et al. Study on the extraction of cinnamon oil from cinnamon leaf by hydro-diffusion distillation method[J]. Applied Chemical Industry, 2014, 43 (6): 1047- 1049.
11	张笮晦, 钱信怡, 黄广智, 等. 不同生长环境和树龄肉桂的出油率、挥发油成分及抑菌活性研究[J]. 中草药, 2019, 50 (12): 2990- 2996.
	ZHANG Z H , QIAN X Y , HUANG G Z , et al. Oil yield, components and antimicrobial activity of volatile oils of Cinnamomi cortex from different growth environments and growth years[J]. Chinese Traditional and Herbal Drugs, 2019, 50 (12): 2990- 2996.
12	张笮晦, 钱信怡, 黄广智, 等. 肉桂和同源根皮不同极性部位的成分分析及其抑菌效果[J]. 林产化学与工业, 2019, 39 (3): 115- 122.
	ZHANG Z H , QIAN X Y , HUANG G Z , et al. Component and antibacterial activity of different polar parts of cinnamon and homologous root bark[J]. Chemistry and Industry of Forest Products, 2019, 39 (3): 115- 122.
13	张笮晦, 李石兰, 童永清, 等. 肉桂叶生物转化的放大实验及产物的抑菌活性研究[J]. 大众科技, 2018, 20 (8): 83- 85, 89.
	ZHANG Z H , LI S L , TONG Y Q , et al. Studies on magnifying experiment of biotransformation of Cinnamomum leaves and antimicrobial activity of the product[J]. Popular Science & Technology, 2018, 20 (8): 83- 85, 89.
14	朱羽尧, 钱骅, 张琪瑶, 等. 肉桂不同植物部位精油成分分析及抑菌活性研究[J]. 中国野生植物资源, 2014, 33 (6): 1- 5.
	ZHU Y Y , QIAN H , ZHANG Q Y , et al. Chemical components and antimicrobial activity of essential oil extracted from five different parts of Cinnamomum cassia[J]. Chinese Wild Plant Resources, 2014, 33 (6): 1- 5.
15	莫开林, 费世民, 吴斌, 等. 时空分布对油樟精油含量的影响研究[J]. 四川林业科技, 2015, 36 (6): 93- 94,26.
	MO K L , FEI S M , WU B , et al. The influence of temporal and spatial distribution on essential oil content in Cinnamomum longepaniculatum[J]. Journal of Sichuan Forestry Science and Technology, 2015, 36 (6): 93- 94,26.

编号 No.	树龄/a tree age	坡向 slope aspect	种植密度¹⁾/(株·dam^-2) planting density/(tree·dam^-2)
1	5	南坡south slope	52~67(G)
2	10	南坡south slope	52~67(G)
3	15	南坡south slope	30~45(S)
4	15	南坡south slope	75~90(C)
5	15	南坡south slope	52~67(G)
6	15	北坡north slope	52~67(G)
7	20	南坡south slope	52~67(G)

样品 samples	抑菌圈直径antibacterial circle diameter/mm
样品 samples	金黄色葡萄球菌S.aureus	大肠杆菌E.coli	绿脓杆菌P. aeruginosa
No.1	35.83±1.24^d	29.40±1.49^ab	23.89±1.44^e
No.2	33.04±0.80^c	31.72±1.28^bc	21.56±0.30^d
No.3	26.92±0.54^b	30.48±1.54^b	18.62±0.36^b
No.4	37.09±0.72^de	34.31±0.99^c	20.65±0.91^cd
No.5	22.57±1.08^a	33.81±1.92^c	20.21±0.10^c
No.6	26.39±0.33^b	27.58±0.51^a	16.39±0.89^a
No.7	39.63±1.84^e	31.79±0.35^bc	20.01±0.51^c
空白对照blank	0	0	0
庆大霉素gentamicin	19.96±0.99	27.59±0.17	20.88±0.30

[1]	XU Hao, ZHANG Ning, YANG Jing, ZHAO Jian, XIE Jingcong, JIANG Jianchun. I-Optimal Mixture Design of Compound Edible Fungi Inoculants for Bioconversion of Cinnamon Leaves into Feeding Stuff [J]. Chemistry and Industry of Forest Products, 2018, 38(5): 122-128.
[2]	CHEN Lu;WANG Yi-wang;LUO Jin-yue;. Synthesis of Cinnamaldehyde Catalyzed by Solid Superbase Na₂CO₃/γ-Al₂O₃ [J]. , 2013, 33(6): 91-94.
[3]	JIA Wei-hong;JIA Wei-hong;RAO Xiao-ping;SONG Zhan-qian. Antimicrobial Activities of Rosin-based Gemini Cationic Surfactants [J]. , 2010, 30(1): 1-4.
[4]	DENG Guo-bin;ZHANG Xiao-long;WANG Yan-yun;LIN Yu;CHEN Xiao-lan. Chemical Composition and Antimicrobial Activity of the Essential Oil of Iris pallida Lam [J]. , 2008, 28(3): 39-44.
[5]	JI Zhi-ping;SU Yin-quan. Study on Antimicrobial Activities of Extracts from Eucommia ulmoides Oliv. Leaves [J]. , 2008, 28(2): 63-66.
[6]	ZHONG Rui-min;XIAO Zi-jun;ZHANG Zhen-ming;LIU Jian-nan;WANG Yu-mei. Composition and Antimicrobial Activity of Essential Oil from Foeniculum vulgare Mill. Seeds [J]. , 2007, 27(6): 36-40.
[7]	JI Zhi-ping;SU Yin-quan;LV Ping-hui;Zhang Cun-li. Studies on Chemical Constituents and Antimicrobial Activities of Persimmon Leaves [J]. , 2006, 26(04): 87-91,9.
[8]	LIANG Hong-jun;LIU Xiong-min;LI Wei-guang;YI Feng-ping;LI Piao-ying . COMPETITIVE REACTIVITIES OF STYRENE,ANETHOLE AND CINNAMALDEHYDE WITH OZONE [J]. , 2002, 22(1): 39-42.

Effects of Growth Conditions on Content, Components and Antimicrobial Activity of Volatile Oil of Cinnamon Leaves

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