蓝靛果籽油提取工艺优化及其脂肪酸组成分析

doi:10.3969/j.issn.0253-2417.2020.06.005

Abstract

Abstract:

The microwave-assisted extraction method was selected to extract blue honeysuckle(Lonicera caerulea var.enulis Turcz et Herd) seed oil and the fatty acid composition of the seed oil was analyzed. Single factor experiments were carried out on extraction time, liquid-solid ratio, extraction temperature, and microwave power. Then, the process for microwave-assisted alcohol extraction of L.caerulea seed oil was optimized by response surface methodology based on the single factor experiments. The results indicated that the optimal extraction conditions were as follows: extraction time 23 min, liquid-to-solid ratio 23:1(mL:g), extraction temperature 50 ℃ and microwave power 500 W, and the yield of L.caerulea seed oil was (26.0±0.05)% under these conditions. The composition of the L.caerulea seed oil was analyzed by GC-MS and 7 fatty acids were identified as the main components, most of which were two conjugated linoleic acids, cis-9, trans-11-octadecadienoate and trans-10, cis-12-octadecadienoate and the relative contents were 23.49% and 21.33%, respectively.

Key words: blue honeysuckle seed oil, microwave-assisted extraction, GC-MS, response surface methodology

CLC Number:

TQ35
TS225.1

Yiwei JIANG, Na GUO, Zhiguo LIU, Chunjian ZHAO, Yujie FU, Xiqing WANG. Optimization of Extraction Technology of Blue Honeysuckle Seed Oil and Analysis of Its Fatty Acid Composition[J]. Chemistry and Industry of Forest Products, 2020, 40(6): 30-36.

Figures/Tables 6

Fig.1

Table 1

Table 2

Fig.2

Fig.3

Table 3

References 19

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方差来源 sources of variance	平方和 sum of squares	自由度 freedom degree	均方 mean square	F值 F value	P值 P value	显著性 significance
模型model	89.27	9	9.92	111.00	0.0001	**
X₁	14.04	1	14.04	157.18	0.0001	**
X₂	22.11	1	22.11	247.45	0.0001	**
X₃	7.41	1	7.41	82.94	0.0001	**
X₁X₂	0.000	1	0.000	0.000	1.0000
X₁X₃	2.25	1	2.25	25.18	0.0015	**
X₂X₃	0.30	1	0.30	3.39	0.1084
X₁²	18.52	1	18.52	207.31	0.0001	**
X₂²	11.08	1	11.08	124.04	0.0001	**
X₃²	9.13	1	9.13	102.17	0.0001	**
残差residual	0.63	7	0.089
失拟项lack of fit	0.078	3	0.026	0.19	0.8991
纯误差pure error	0.55	4	0.14
总离差total dispersion	89.89	16

序号 No.	分子式 molecular formula	脂肪酸 fatty acid	GC含量GC content/%
序号 No.	分子式 molecular formula	脂肪酸 fatty acid	微波辅助提取法 microwave-assisted extraction	索氏提取法 soxhlet extraction
1	C₁₈H₃₂O₂	9，顺-11，反-十八碳二烯酸 cis-9, trans-11-octadecadienoate	23.485	23.689
2	C₁₈H₃₂O₂	10，反-12，顺-十八碳二烯酸 trans-10, cis-12-octadecadienoate	21.328	17.915
3	C₁₈H₃₄O₂	十八碳烯酸 13-octadecenoate	13.592	12.742
4	C₁₈H₃₄O₂	反式-13-十八碳烯酸 trans-13-octadecenoate	5.184	3.574
5	C₁₈H₃₂O₂	5，9，12-十八碳三烯酸 5, 9, 12-octadecatrienoate	3.628	3.201
6	C₂₀H₃₄O₂	9，12，15-二十碳三烯酸 9, 12, 15-eicosatrienoate	2.097	3.074
7	C₂₀H₃₄O₂	7，11，14-二十碳三烯酸 7, 11, 14-eicosatrienoate	1.460	1.570
	单不饱和脂肪酸monounsaturated fatty acid		18.776	16.316
	多不饱和脂肪酸polyunsaturated fatty acid		51.998	49.449
	总不饱和脂肪酸total saturated fatty acid		70.774	65.765

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Optimization of Extraction Technology of Blue Honeysuckle Seed Oil and Analysis of Its Fatty Acid Composition

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序号 No.	X₁ 提取时间/min extraction time	X₂ 液料比值(mL:g) liquid-to-solid ratio	X₃ 提取温度/℃ extraction temperature	Y 出油率/% yield of oil
1	30	25	50	24.6
2	20	25	60	25.2
3	10	20	40	20.3
4	30	20	40	21.3
5	20	20	50	25.4
6	10	15	50	18.6
7	30	15	50	21.4
8	20	20	50	25.6
9	20	15	40	19.8
10	20	25	40	22.7
11	10	20	60	20.7
12	10	25	50	21.8
13	30	20	60	24.7
14	20	20	50	24.8
15	20	20	50	25.1
16	20	20	50	25.7
17	20	15	60	21.2