圆齿野鸦椿籽油提取工艺及脂肪酸组分分析

doi:10.3969/j.issn.0253-2417.2021.01.008

Abstract

Abstract:

To high-efficiency use the seeds of Euscaphis konishii Hayata, n-hexane and heating reflux extraction was used to extract oil from the seeds of E. konishii, and the effects of extraction time, extraction temperature and liquid/solid ratio of n-hexane and E. konishii seed on extraction rate were investigated. Based on the results of single-factor test, Box-behnken response surface design and data analysis were carried out by using Design-Expert 8.0.6 software to further optimize the reflux extraction process of E. konishii seed oil. The results showed that the order of the factors affecting the extraction rate were extraction time, extraction temperature and liquid/solid ratio.The optimal extraction process was heating reflux extraction time 62.04 min, extraction temperature 80.2 ℃ and liquid/solid ratio 10.14:1(mL: g). The predicted extraction rate under these conditions was 5.84% and the total extraction rate obtained from verifying test was (5.85±0.001)%, which was close to the predicted value and the relative error was 0.17%, indicating that the response surfaced optimized extraction process of E. konishii seed oil was stable and workable. Meanwhile, by analyzing the oil extracted from seed of E. konishii, the yield of kernel oil was (35.6±0.7)% and the yield of seed coat oil was (2.1±0.15)%, 18 fatty acid components were defected, among which the unsaturated fatty acids were 65.52%, and linoleic acid content was up to 45.64%.

Key words: Euscaphis konishii Hayata, response surface methodology, reflux extraction, oleic acid, linoleic acid

CLC Number:

TQ35
TQ91

Shuqin CHEN, Yanyan YE, Yingxuan QUAN, Linhai LI, Lin NI, Shuangquan ZOU. Extraction Process and Components Analysis of Oil from the Seeds of Euscaphis konishii Hayata[J]. Chemistry and Industry of Forest Products, 2021, 41(1): 53-59.

Figures/Tables 5

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方差来源 source of variance	平方和 sum of squares	自由度 degree of freedom	均方 mean square	F值 F value	P值 P value	显著性 significant
模型 model	1.01	9	0.11	18.5	0.0004	**
X₁	0.04	1	0.038	6.26	0.04	*
X₂	0.01	1	0.00551	0.91	0.37
X₃	0.01	1	0.005	0.83	0.39
X₁X₂	0.19	1	0.19	31.31	0.0008	**
X₁X₃	0.01	1	0.0064	1.06	0.34
X₂X₃	0.05	1	0.05	8.01	0.0254	*
X₁²	0.27	1	0.27	45.39	0.0003	**
X₂²	0.26	1	0.26	43.63	0.0003	**
X₃²	0.10	1	0.1	17.34	0.0042	**
残差 residual	0.04	7	0.00604
失拟 lack of fit	0.04	3	0.01	62.77	0.0008	**
误差 pure error	9.00×10^-4	4	0.00022
总计 cor total	1.05	16

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Extraction Process and Components Analysis of Oil from the Seeds of Euscaphis konishii Hayata

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试验编号 No.	X₁ 提取时间/min extraction time	X₂ 提取温度/℃ extraction temperature	X₃ 液料比值 ratio of solid to liquid	提取得率/% extraction yield
1	60	80	10	5.84
2	60	80	10	5.86
3	60	80	10	5.84
4	60	80	10	5.83
5	60	80	10	5.82
6	75	70	10	5.26
7	45	70	10	5.55
8	60	70	12	5.27
9	60	70	8	5.55
10	75	80	12	5.53
11	45	80	8	5.24
12	45	80	12	5.48
13	75	80	8	5.45
14	75	90	10	5.55
15	45	90	10	4.97
16	60	90	12	5.53
17	60	90	8	5.37