O/W型山苍子精油微乳的制备及其性能研究

doi:10.3969/j.issn.0253-2417.2021.01.010

Abstract

Abstract:

A microemulsion of Litsea cubeba(Lour.) Pers. oil was prepared by adding water. The influence of different surfactant and cosurfactant-types, the mass ratio of surfactant to cosurfactant(K_m), and preparation temperature on the formation of water-in-oil(O/W) type microemulsion of L. cubeba oil were investigated using a single factor method, and through orthogonal experiments, the optimum preparation process of O/W type L. cubeba oil microemulsion was determined as follows: Triton X-100 as surfactant, absolute alcohol as cosurfactant, K_mvalue was equal to 1, and preparation temperature was set to 25 ℃. Under the best preparation conditions, the ratio of the mixed surfactant and the essential oil of L. cubeba was 9∶1, and the water content was 70% for sample 1. The average particle diameter, polydispersion coefficient, conductivity and pH value of sample 1 were 13.43 nm, 0.125, 83 mS/m, and 5.82, respectively. The performance test and analysis of sample 1 showed that there was no change in the appearance of the microemulsion when it was centrifuged at the rate of 1 000 to 4 000 r/min, which could prove that the centrifugal stability was better than essential oil. Adding the same amount of methylene blue aqueous solution to sample 1 and essential oil, its diffusion rate in sample 1 was significantly faster than that in the essential oil, which proved that the water solubility of microemulsion was significantly better than that of the essential oil.After 12 h of sustained-release, the content of sustained-release citral in the microemulsion was significantly less than that of the sustained-release citral in the essential oil, indicating that the sustained release of the microemulsion was better than that of the essential oil itself. The results of 1, 1-diphenyl-2-picrylhydrazyl free radical(DPPH·) and 2, 2'-azinobis-(3-ethylbenzthiazoline-6-sulphonate) radical(·ABTS⁺) scavenging experiments indicated that DPPH· and·ABTS⁺ scavenging rates of sample 1 of L. cubeba essential oil microemulsion were significantly higher than those of essential oil in the concentration range of 10-30 g/L. The DPPH·and·ABTS⁺ scavenging rates of the L. cubeba essential oil microemulsion sample 1 at 30 g/L concentration were 95% and 54.7%, while those of L. cubeba essential oil were only 44.79% and 16.4%, respectively, indicating that L. cubeba essential oil prepared as microemulsion could significantly improve its antioxidant property, and the anti-oxidation property of microemulsion to DPPH· was significantly better than that of·ABTS⁺.

Key words: Litsea cubeba essential oil, microemulsion, preparation conditions, antioxidant activity

CLC Number:

TQ35
TS221

Chen CHENG, Hongyan SI, Zongde WANG, Shengliang LIAO, Chenyang ZOU, Peng WANG. Preparation Conditions and Properties of O/W Type Litsea cubeba Oil Microemulsion[J]. Chemistry and Industry of Forest Products, 2021, 41(1): 67-76.

Figures/Tables 12

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References 16

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序号 No.	助表面活性剂 cosurfactant	表面活性剂 surfactant	K_m值 K_m value	制备温度/℃ preparation temperature	微乳区面积/总面积 microemulsion area/total area
1	无水乙醇absolut alcohol	Triton X-100	1	15	163.19/300
2	无水乙醇absolut alcohol	EL-30	2	25	106.18/300
3	无水乙醇absolut alcohol	EL-35	3	35	77.66/300
4	PEG-400	Triton X-100	2	35	19.01/300
5	PEG-400	EL-30	3	15	0.14/300
6	PEG-400	EL-35	1	25	0.07/300
7	1, 2丙二醇1, 2-propanediol	Triton X-100	3	25	129.99/300
8	1, 2丙二醇1, 2-propanediol	EL-30	1	35	95.28/300
9	1, 2丙二醇1, 2-propanediol	EL-35	2	15	39.47/300
k₁	115.68	104.06	86.18	67.60
k₂	6.41	67.20	54.89	78.75
k₃	88.25	39.07	69.26	63.98
R	109.27	64.99	31.29	14.77

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Preparation Conditions and Properties of O/W Type Litsea cubeba Oil Microemulsion

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序号 No.	含水率/% water content	温度/℃ temperature	外观 appearance	序号 No.	含水率/% water content	温度/℃ temperature	外观 appearance
1	70	15	澄清clear	21	90	15	澄清clear
2	70	25	澄清clear	22	90	25	澄清clear
3	70	35	浑浊muddy	23	90	35	浑浊muddy
4	70	45	浑浊muddy	24	90	45	浑浊muddy
5	80	15	澄清clear	25	60	15	澄清clear
6	80	25	澄清clear	26	60	25	澄清clear
7	80	35	浑浊muddy	27	60	35	澄清clear
8	80	45	浑浊muddy	28	60	45	澄清clear
9	90	15	澄清clear	29	70	15	澄清clear
10	90	25	澄清clear	30	70	25	澄清clear
11	90	35	浑浊muddy	31	70	35	澄清clear
12	90	45	浑浊muddy	32	70	45	澄清clear
13	70	15	澄清clear	33	80	15	澄清clear
14	70	25	澄清clear	34	80	25	澄清clear
15	70	35	澄清clear	35	80	35	澄清clear
16	70	45	浑浊muddy	36	80	45	澄清clear
17	80	15	澄清clear	37	90	15	澄清clear
18	80	25	澄清clear	38	90	25	澄清clear
19	80	35	浑浊muddy	39	90	35	澄清clear
20	80	45	浑浊muddy	40	90	45	浑浊muddy