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Chemistry and Industry of Forest Products ›› 2021, Vol. 41 ›› Issue (3): 77-84.doi: 10.3969/j.issn.0253-2417.2021.03.011

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Extraction and Anti-inflammatory Effect of Polysaccharides from Euscaphis konishii Hayata Pericarp

Wei HUANG1,2, Mengyun GUO1, Yuqi WANG1, Jinlei LIN1, Yuxiang ZHANG1, Shuangquan ZOU2,*()   

  1. 1. College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350004, China
    2. Engineering Research Institute of Conservation, Utilization of Natural Bioresources, Fuzhou 350004, China
  • Received:2020-08-08 Online:2021-06-28 Published:2021-07-01
  • Contact: Shuangquan ZOU E-mail:zou@fafu.edu.cn

Abstract:

To develop and utilize the polysaccharide resources from Euscaphis konishii Hayata, the extraction technology and anti-inflammatory effect of polysaccharides from E. konishii pericarp were studied. The extraction process was optimized by single-factor experiments and response surface methodology. Results showed that under the conditions of liquid to material ratio of 30:1(mL: g), ultrasonic power of 400 W, extraction at 60℃ for 100 min, the polysaccharide yield could reach up to 29.15%. The crude polysaccharides were separated and purified by DEAE-52 cellulose column chromatography to get a main E. konishii pericarp polysaccharide(PEP). The monosaccharide compositions of PEP were analyzed by HPLC with pre-column derivatization. The results showed that PEP contained ten monosaccharides including mannose 5.65%, glucose 37.06%, galactose 19.51%, fucose 2.24%, xylose 26.10%, ribose 1.33%, gluconose 0.89%, rhamnose 2.33%, galactose aldose 0.68% and arabinose 4.20%. PEP with the mass concentrations of 1-10 g/L inhibited the LPS-induced secretion of inflammatory factors in RAW264.7 macrophages, but had no significant effect on the cell viability, which suggested that PEP possessed anti-inflammatory capacity, and had a good prospect for development and utilization.

Key words: polysaccharides, extraction, monosaccharides composition, anti-inflammatory capacity

CLC Number: