银杏叶色素类化合物的提取分离及结构鉴定

doi:10.3969/j.issn.0253-2417.2023.01.010

Abstract

Abstract:

Ethanol extracts of three samples were prepared from Ginkgo biloba L. fresh leaves(sample 1), dry leaves(sample 2) and old leaves(sample 3). HPLC-DAD-MS method was used to analyze the pigment compounds in three G. biloba leaves samples. The separation, purification and structure identification of ginkgo leaf pigment compounds in sample 2 and sample 3 were conducted by liquid-liquid extraction, silica gel column chromatography, HPLC preparative chromatography, etc. From the three samples, 24 kinds of pigment compounds in G. biloba leaves were preliminarily speculated, including 17 kinds of chlorophyll compounds and 7 kinds of carotenoid compounds. Ten monomer pigment compounds in G. biloba leaves were isolated and purified from ethanol extract of sample 2 and sample 3. They were identified as β-carotene (A), pheophytin a (B), pheophytin b (C), lutein (D), phy-purpurin 18a (E), pyropheophytin a (F), 13²-OH-pheophytin a (G), 13²-OH-pheophytin a' (H), 13²-OH-pheophytin b (I), and 13²-OH-pheophytin b' (J), respectively, by mass spectrometry, ultraviolet spectroscopy, infrared spectroscopy and proton nuclear magnetic resonance spectroscopy combined with literature comparison. Among them, E, F, G, H, I and J were isolated from G. biloba leaves for the first time.

Key words: Ginkgo biloba leaves, pigments, extraction, separation, structure identification

CLC Number:

TQ35

Hong SHEN, Changwei ZHANG, Hongxia CHEN, Ran TAO, Jianxin JIANG, Chengzhang WANG. Extraction, Isolation and Structure Identification of Pigment Compounds from Ginkgo biloba Leaves[J]. Chemistry and Industry of Forest Products, 2023, 43(1): 79-88.

Figures/Tables 4

References 27

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Extraction, Isolation and Structure Identification of Pigment Compounds from Ginkgo biloba Leaves

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