Abstract: When the steam exploded corn stover was hydrolyzed by cellulase at substrate loading of 100 g/L and cellulase dosage of 20 FPIU/g-cellulose, β-glucosidase dosage of 3IU/g-cellulose for 48 h, the hydrolysis yield was 74.89%. Following solid-liquid separation, a liquid fermentation of concentrated supernatant was performed, the glucose consumption yield and ethanol yield were 99.56% and 94.50%, respectively. Variable temperature fermentation of enzymatic hydrolysis residue was carried out at yeast loading of 2.0×10⁶ cfu/g enzymatic hydrolysis residue, applying 30℃ for the first 24 h and 36℃ for the next 42 h. It was found that 11.59 g ethanol was obtained from 474.3 g enzymatic hydrolysis residue. 175 g steam exploded corn stover was bio-refinied through the intergrated processes containing lower substrate hydrolysis, higher glucose concentration fermentation and variable temperature fermentation of enzymatic hydrolysis residue process. Finally, 36.49 g ethanol was obtained, and the pretreated corn stover-to-ethanol conversion yield was 0.209 g/g.

Key words: corn stover, enzymatic hydrolysis residue, two-stage solid state fermentation, integrated process