Welcome to Chemistry and Industry of Forest Products,

›› 2009, Vol. 29 ›› Issue (1): 68-72.

• 研究报告 • Previous Articles     Next Articles

Extraction, Separation, Purification and Structural Analysis of Polysaccharide from Stenoloma chusanum(Linn.)Ching

LUO Ya-jun1, XIAO Xin-feng2, WANG Zhao-li3   

  1. 1. Department of Chemistry and Chemical Engineering, Mianyang Normal University, Mianyang 621000, China;2. College of Chemical and Environmental Engineering, Shandong University of Science & Technology, Qingdao 261000, China;3. Chengdu Academy of Environmental Protection, Chengdu 610065, China
  • Received:2008-01-23 Revised:1900-01-01 Online:2009-02-28 Published:2009-02-28

Abstract: Water-soluble polysaccharide in Stenoloma chusanum(Linn.)Ching is extracted by means of hot water. Polysaccharide is purified by enzyme treatment and Seveg method to remove protein, then decolorized by 10% H2O2 to remove pigment, followed by DEAE-cellulose column chromatography. Polysaccharide is shown to be a single homogeneous component by PC and Sephadex G-100 column chromatography, and their structural composition was studied by means of permeation chromatography (GPC),TLC, GC-MS, UV, IR, etc.. The average molecular mass of polysaccharide is 8.6×104u. Polysaccharide is composed of glucose, arabinose and uronic acid. There is no absorption peak of protein and nucleic acid at 280 and 260nm. Polysaccharide of this plant is a non-proteinous single polysaccharide containing β-glucopyranose.

Key words: Stenoloma chusanum(Linn.)Ching, polysaccharides, separation and purification, structural analysis

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