›› 2009, Vol. 29 ›› Issue (1): 79-82.
• 研究报告 • Previous Articles Next Articles
SHI Wei-guo, ZU Yuan-gang, ZHAO Chun-jian, YANG Lei, LI Jia-hui
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Abstract: The method of homogenated extraction and HPLC determination of camptothecine in seed and leaf of common campto-theca(Camptotheca acuminata) were studied,and comparisons of this extraction method with other methods such as ultra sonic extraction,refluxing, soaking and shaking in water bath were conducted.For the homogenated extraction,the optimum technology parameters were found as following: homogenation for 8 min with 55% ethanol at a ratio of material to liquid of 1:15 (g:mL).By using the optimized parameters,the extraction yields of camptothecine from C. acuminata seeds and leaves were 0.0801% and 0.0679%,respectively.Moreover,homogenated extraction required less extraction time and had high yield,which was an efficient method for extracting camptothecine.
Key words: homogenation, Camptotheca acuminata seed, C. acuminata leaf, camptothecine
CLC Number:
TQ351.0
R284.2
SHI Wei-guo;ZU Yuan-gang;ZHAO Chun-jian;YANG Lei;LI Jia-hui. Homogenated Extraction of Camptothecine from Camptotheca acuminata Decne. Seeds and Leaves[J]. , 2009, 29(1): 79-82.
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