%A SONG Ruyuan, ZHAO Luyang, XIA Zihua, WU Weibing, JING Yi, DAI Hongqi %T Preparation and Optical Properties of Fluorescent Probes Based on Cellulose Nanocrystals %0 Journal Article %D 2018 %J Chemistry and Industry of Forest Products %R 10.3969/j.issn.0253-2417.2018.06.007 %P 51-58 %V 38 %N 6 %U {http://www.cifp.ac.cn/CN/abstract/article_2517.shtml} %8 2018-12-28 %X Multifunctional fluorescent cellulose nanocrystals (F-CNC) was prepared by the covalently bonded carboxylated cellulose nanocrystals (C-CNC) with 4-((2-aminoethyl)amino)-9-methyl-1,8-naphthalimide (AANI). Then, F-CNC was characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD) and infrared spectroscopy (FT-IR), respectively. The optical properties and fluorescence-labeled imaging properties of F-CNC were further analyzed. The characterization results showed that AANI was successfully attached to the surface of C-CNC and the CNC substrate improved the hydrophilicity of the dye. The maximum solubility of AANI in H2O was 0.8 mmol/L, while the concentration of dye in the prepared F-CNC mother liquor was 1.2 mmol/L. F-CNC was stable in aqueous solution and exhibited a good bright yellow fluorescence emission property. At the same time, F-CNC retained the rod shape of C-CNC and had little change in size. The crystallinity and crystal structure didn't changed significantly. The crystallinities of C-CNC and F-CNC were 61.2% and 67.7%, respectively. The decrease of the solvent polarity compressed the double layer of the F-CNC and caused the aggregation of the F-CNC to some extent, resulting in higher fluorescence sensitivity of F-CNC to solvent polarity than that of AANI. The results of fluorescent labeling showed that when the mass concentrations of F-CNC were 0, 3.375×10-4 and 6.75×10-4 g/L, the survival rates of cells after incubated for 10 h were 49.08%, 44.21% and 40.71%, respectively. F-CNC had good fluorescent-labeled imaging performance for Hela cells, and had low toxicity to cells.