%A CHEN Xueyan, CHU Qiulu, WANG Jing, ZHANG Xiaodong, SHI Aiping %T Effects of Removal of Lignin via Two-stage Pretreatment on the Enzymatic Hydrolysis of Poplar %0 Journal Article %D 2019 %J Chemistry and Industry of Forest Products %R 10.3969/j.issn.0253-2417.2019.05.010 %P 73-79 %V 39 %N 5 %U {http://www.cifp.ac.cn/CN/abstract/article_2608.shtml} %8 2019-10-28 %X The effects of lignin fractionation via two-stage pretreatment on the surface morphology, physicochemical properties and enzymatic hydrolysis of the poplar chips were investigated. The results showed that, comparing to one-stage steam explosion(SE) pretreatment, alkaline sulfonation-steam explosion(AS-SE) and alkaline oxidation-steam explosion(AO-SE) pretreatment both led to the removal of around 50% lignin from biomass, while incorporating extensive hydrophilic acid groups including sulfonic and carboxylic groups into substrates. As a result, cellulose accessibility was increased to 132.04 and 119.10 mg/g, respectively. Besides, FT-IR spectrum confirmed the incorporation of hydroxyl, carboxylic and sulfonic groups into lignin after AS and AO pretreatments. SEM images showed the cracked and corroded surface morphology after the two-stage pretreatments for lignin fractionation. After the AS-SE pretreatment, 81.09% of the cellulose in pretreated solid could be converted to glucose, while the yield of biomass saccharification was improved to 73.72%. These results indicated that lignin fractionation via two-stage pretreatment led to the selectively removal and modification of lignin, resulting in the change of the physicochemical properties and surface morphology of lignin, and favoring the enzymatic hydrolysis of cellulose.