In order to extract bioactive components and gutta-percha effectively from leaves of Eucommia ulmoides Oliver, the successive extractions of the leaves by hot water, alkali, and enzyme-petroleum ether were used to obtain the extractum, polysaccharides(water-soluble polysaccharides and alkali-extracted polysaccharides) and gutta-percha, respectively. Effects of solid to liquid ratio and temperature on yields of bioactive components were investigated. Meanwhile, the effects of time and type of enzymes on enzymatic hydrolysis of the residues were discussed. Finally, the ferric reducing/antioxidant power and the scavenging DPPH radical activity of bioactive constituents, water-soluble and alkali-extracted polysaccharides were determined, and the molecular weights of gutta- percha were determined. The results showed that the highest yields of flavonoids and chlorogenic acid were 50.08 mg/g and 6.83 mg/g with solid to liquid ratio of 1:10 (g:mL)at 80 ℃. The water-soluble and alkali-extracted polysaccharides and extractum from E. ulmoides Oliver exhibited good antioxidant activity, in which the antioxidant activity of extractum was the highest. The total antioxidant activity of 5 g/L extractum was 4.01 mmol/L, and the scavenging rate of DPPH radical at 300 mg/L was 94.46%, which was the same as that of Vc. The hydrolysis rates of glucan and xylan were the highest when water-extracted residues of E. ulmoides leaves were hydrolysed for 48 h, which were 86.77% and 15.62%, respectively. The yields of extractum, water-soluble polysaccharides, alkali-extracted polysaccharides and gutta-pereha were 8.0%, 13.2%, 9.8%, 1.86%, respectively. The weight average relative molecular mass (Mw) and the number average relative molecular mass (Mn) of gutta-pereha were 330 398 and 84 526, respectively, which were higher than the molecular weights of polysaccharides. Meanwhile, Mw/Mn=3.91, and the dispersity of extractum was lower than that of polysaccharides.