Methyleugenol was prepared from eugenol in the presence of potassium carbonate (K ₂CO ₃), using dimethyl carbonate(DMC) as methylating agent instead of the conventional toxic agents. Using the yield of methyleugenol as the evalution index, central composite design process was used to optimize the methylation reaction of eugenol and DMC. An optimum reaction condition for the preparation of the targeted product was as follows, reaction temperature 155 ℃, the amount of K ₂CO ₃ 6.12 %(mea-sured by the molar ratio of eugenol), the molar ratio of DMC and eugenol 4.3:1 and reaction time 6 h. Under this condition the conversion rate reached 96.16 %. Meanwhile, repeated experiments showed that the reaction process was easy to be controlled and excellent repetitiveness can be acquired. GC-MS and IR spectral analysis were employed to confirm the structure of the product.

Diels-Alder reaction between β-myrcene and maleic anhydride catalyzed by SO ₄ ^2-/TiO ₂-ZrO ₂ solid superacid was investigated. By using GC, GC-MS and IR, main product in this reaction is confirmed to be 4-(4-methyl-3-pentenyl)-4-cyclohexene-1,2-dicarboxylic anhydride. The result shows that this catalyst has high catalytic activity and good selectivity to the Diels-Alder reaction between β-myrcene and maleic anhydride. The effects of catalyst to its catalytic activity are investigated. The result also shows that the optimum conditions for the preparation of SO ₄ ^2-/TiO ₂-ZrO ₂ catalysts are as follows: n(Ti): n(Zr) 1:1, calcination temperature at 450℃. Under these conditions, the optimum conditions for the Diels-Alder reaction are as follows: n( β-myrcene): n(maleic anhydride) 1:1,reaction time 4 h, reaction temperature 60℃, and the ratio of solid superacids to β-myrcene mass part 1%, the β-myrcene conversion is 96.5%, and the 4-(4-methyl-3-pentenyl)-4-cyclohexene-1,2-dicarboxylic anhydride selectivity is 94.0%, or yield is 90.7%. Furthermore, the effect of storage time of the catalysts on the reaction and the experiments on regeneration of catalysts are carried out.

Thermal decomposing curves of gallic acid in different heating speeds were obtained in the nitrogen atmosphere by TG-DTG techniques. Two analysis methods of Achar and Coats-Redfern were used to speculate the probable mechanism of thermal decomposing reaction and the kinetic parameters. The shelf life of gallic acid at room temperature was calculated by the kinetic parameters of the first stage. With the increasing of the heating rate, thermal decomposing tempreture of gallic acid rose. The most probable kinetic mechanisms of the three-stage thermal decomposition were all chemical reactions, and the corresponding mechanisms followed on reaction order. In accordance with the data of TG and Gaussian, one molecule of gallic acid lost 0.5 atom O at the fisrt-stage, and lost another 0.5 atom O and a molecule CO ₂ at the second stage. The shelf life of gallic acid at room temperature was about 1.5-2 years.

The present application of rosin in wood preservation includes reducing moisture absorption of wood, improving water-based wood preservatives fixation, and preparing active rosin derivatives to wood-decay fungi. Wood-decay resitance can be improved by high concentration rosin emulsion treatment. Rosin emulsion treatment at even a low concentration could well fix leachable copper salts in wood. Rosin amine and quaternary ammonium salt derivatives could protect wood from fungal attack. Effects of imidazoline and other bioactive derivatives need more tests.

Inositol, known as cyclohexanehexol, has very important biological functions in the growth of plants. The myo-inositol is the most abundant isomer of inositol in plants which is formed by glucose cyclization catalyzed by hexokinase, myo-inositol-1-phosphate synthase and alkaline phosphatase. It takes part in some vital oxidation pathway in plants. Cell wall formation has a close relationship with the metabolism of myo-inositol. Xylan and pectin are important contents of cell wall polysaccharides can be formed by myo-inositol oxidation pathway. This provides important basis for the study of the linkage between xylan and pectin and lignin in plant cell wall by isotopic tracer method. This also contributes to the study of plant fiber chemistry and pulp and paper science.

Benzaldehyde is used as a raw material, to synthesize cinnamaldehyde from acetaldehyde in catalysis by aldol condensation reaction. The conditions of the catalytic reaction were discussed. The product was characterized by GC-MS,IR and ¹H NMR. The results show that: When the catalyst is Na ₂CO ₃/ γ-Al ₂O ₃, the molar proportion of benzaldehyde and acetaldehyde is 1.3:1, the reaction temperature is 30 ℃, reaction time is 1 h, the amount of catalyst is 1 g (according to 11.6 g 40% acetaldehyde), the yield of cinnamaldehyde reaches 40.9%.

Advances of research on preparation, modification and application of nanocellulose whiskers(NCW) are reviewed. NCW are produced from natural cellulose by sulfuric-acid hydrolysis. Recent progress of research on hydrolysis process, preparation methods and conditions, characterization of properties of NCW, surface modification methods and the application of NCW in the fields of fine chemical and material science,etc. are mainly introduced.

Phosphoric acid activation is a principal method of the chemical activations to produce activated carbons, and has a history of around 30 years in the laboratory-scale investigation and the industrial application. In this article, the author systemically reviewed the roles that phosphoric acid played in the process of H ₃PO ₄ activation for the first time. From the chemical point of view, phosphoric acid played in promoting hydrolysis of biopolymers in lignocellulosic materials (1), catalyzing dehydration of biopolymers (2) and aromatization reaction of carbon atoms (3), initiating crosslink reaction with biopolymers (4), and evolution of pores (5). Moreover, three sequential stages were considered to be necessary for the deep incorporation of H ₃PO ₄ solution into the interior of botanic structures, which include fast diffusion, hydrolysis and post diffusion. Meantime, the roles that the activating agents play in H ₃PO ₄ activation and ZnCl ₂ activation are compared.

The aim of this study was to determine the chemical composition of Synsepalum dulcificum leaf essential oil obtained by steam distillation. The in vitro antibacterial activity and antitumoral activity of this essential oil was characterized. Sixty-eight compositions were separated. Among them 44 components were identified and represented 92.14% of the total detected constituents. The major chemical compounds of leaf essential oil were spathulenol (24.194%), limonene (15.805%), diisooctyl phthalate (12.402%), dibutyl phthalate (10.326%), palmitic acid (4.865%) and linalool (2.139%). The result of the antimicrobial assay showed that the essential oil displayed varying degrees of antibacterial activity against all tested bacterial except for Pseudomonas aeruginosa, along with minimum inhibition concentration(MIC)values from 39.06 to 252.15 mg/L. In addition, the antitumoral activity using MTT assay of the leaf essential oil exhibited this oil was effective against human K562 cancer cell line in a dose dependent manner. Its inhibition concentration 50% (IC ₅₀) value was 13.5 mg/L.

Nanocellulose crystal was prepared by sulfuric acid hydrolysis. The effects of sulfuric acid mass fraction, temperature and time on the yield of nanocellulose crystal were optimized with response surface methodology. The mathematical model was established using the Design-Expert software. The effect of various factors and their interactions were analyzed as well. The results showed that quadratic model was the best model to describe the relationship between the yield of nanocellulose crystal and the factors. The determination coefficient and adjusted determination coefficient were 99.31 % and 98.43 %, respectively. The interactions between sulfuric acid concentration and temperature, sulfuric acid concentration and time, temperature and time were significant. When sulfuric acid concentration, temperature and time corresponded to 54 %, 52 ℃ and 125 min, the yield of nanocellulose crystal would reach 69.31 %. It is the highest value in this investigation.

Maleic rosin acid was prepared using rosin and maleic anhydride as raw materials. Effects of different reaction conditions on the reaction were investigated by single factor experimentation, and the optimum conditions were found to be as follows: mole ratio of maleic anhydride to rosin 1.1∶1, reaction temperature 190 ℃, reaction time 4 h. The structure of maleopimaric acid anhydride was characterized by IR and single crystal X-ray diffraction. It was found that the configuration of maleopimaric acid anhydride was the classical type of diterpene compounds. The prepared maleic rosin was hydrolyzed and purified by potassium salt method to obtain maleic rosin acid with purity of 95.3% and yield of 81.5%. The structure of maleic rosin acid was verified by means of IR, ¹H NMR, ¹³C NMR and MS.

Biomass energy is an important part of renewable energy.High effective utilization and development of biomass energy has positive effects on solving energy and environment problems.Since 1970's,in countries worldwide,especially in advanced countries,extra attentions have been paid to conduct researches on the application technique of biomass energy and a lot of research progress have been achieved,some of which have been used in industrial scale.In this paper,the progress of research and development in domestic and abroad,concerning biomass solidification,liquefaction,gasification and direct combustion technique,are summarized.According to our country's situation,proposals on research and development prospect are put forward.

The interaction between ursolic acid (UA) and serum albumin, namely bovine serum albumin (BSA) and human serum albumin (HSA) respectively has been studied by fluorescence spectroscopy. Based on the method of florescence quenching, the binding constants ( K) for UA with BSA/HSA were determined to be K _UA-BSA=0.335 7×10 ⁵ L/mol and K _UA-HSA=0.244 3×10 ⁵ L/mol, respectively. The binding distance( r)and energy-transfer efficiency( E)between UA and BSA/HSA were also obtained by virtue of the Fōrster theory of non-radiation energy transfer to be as follows: r _UA-BSA=0.73 nm, r _UA-HSA=0.88 nm, E _UA-BSA=0.17, E _UA-HSA=0.18. The effect of UA acting on the conformation of BSA/HSA analyzed by synchronous fluorescence spectroscopy was not significant. The unfolding procedure of BSA/HSA induced by UA was analyzed by fluorescence phase diagram to be a two-state model. In the presence of Ni(Ⅱ), interaction between UA and BSA/HSA was explored to be somewhat different.

The research progress on the phytochemistry, pharmacology, as well as antitumoral activity and mechanism of liriodenine was reviewed, which is a kind of typical oxoaporphine alkaloid. Liriodenine widely distributes in many natural plants of a series of families and genera, but the content is very low. Liriodenine exhibits extensive pharmacological activities, such as antitumoral, antibacterial, antifungal and trypanocidal activities, as well as anti-alzheimer's disease. It is seen that the significant antitumoral activity should be ascribed to the hyperconjugational planar structure of oxoaporphine as parent nucleus. Moreover, the pro-phase research progress as the rise of medicinal inorganic chemistry of liriodenine was also briefly introduced based on the authors' achieved research efforts. It could provide new strategy for the expanded research and application of liriodenine.

The domestic and international research progress of activated carbon production and application status in recent twenty years were reviewed. The development of chemical activation and physical activation of activated carbon were summarized, and the latest breakthrough on the integrated production process of physical and chemical activation was introduced. The pollution-free, low consumption and pretreatment production technology of activated carbon industrial production and the regeneration production technology of adsorption-saturated activated carbon were briefly described. Meanwhile, the research progresses of its application on gas adsorption, liquid adsorption and catalyst carrier were discussed, too. The existing problems of production and application technology of activated carbon were put forward, the development of activated carbon industry outlet and solutions were clarified, and the future research directions of activated carbon were further pointed out.

Anthocyanin is a kind of flavonoid which mainly exists in plants. It has strong biological activities such as anti-cancer and anti-oxidation, and is widely used in the field of nutrition and health care. Most anthocyanins are extracted directly from plants by physical or chemical means, but the yield is low, and the extracted anthocyanins are mostly mixtures due to the restrictions of time, region and season. Biosynthesis of anthocyanins have attracted much attention by researchers worldwide in recent years. As the biosynthesis of anthocyanins can be controlled artificially and the purity of the obtained products is high, it has been widely studied. Anthocyanins cannot exist stably in the environment, and need to be modified by glycosylation, acylation and methylation to increase their stability. The modified anthocyanins can be synthesized by microorganisms and significant color changes can be seen in the medium. The biosynthesis and modification of anthocyanins were reviewed in this paper, and the preparation technology of anthocyanin synthesis from plants and microorganisms was briefly introduced, the factors affecting anthocyanin synthesis were analyzed, and finally the future research direction of anthocyanin was predicted.

A convenient and industrializable new method of synthesizing 1,8-octanediol was studied. 1,8-Octanedioic acid dimethyl ester was obtained at high yield, using hexamethylene dicyanide and methanol as raw material catalyzed by concentrated sulfuric acid, followed by the reduction of 1,8-octanedioic acid dimethyl ester via sodium borohydride in a NaBH ₄-methanol-tetrahydrofuran solvent system to obtain 1,8-octanediol. Effects of the amount of NaBH ₄ and reaction time on the overall yield of 1,8-octanediol were investigated. The optimum reaction conditions were as follows: molar ratio of NaBH ₄ to 1,8-octanedioic acid dimethyl ester 5:5.1, reaction time 8.5h, the yield of 1,8-octanediol was 97.2%.

Corn stalk was pretreated by four kinds of pretreatment methods, namely, dilute acid, dilute acid refining, neutral steam explosion and dilute acid steam explosion. The effects of pretreatment on enzymatic hydrolysis and yield of fermentable su-gars were investigated. The components of pretreated corn stalk, sugars in pre-hydrolyzate, carbohydrate and lignin degradation products in pre-hydrolyate were also analyzed. After 100 g corn stalk was pretreated by dilute acid, dilute acid refining, neutral steam explosion and dilute acid steam explosion followed by washing, the cellulose content -dropped from 37.17g to 33.96, 33.54, 32.63 and 32.88 g, respectively; the xylan content declined from 22.84 g to 2.77, 2.47, 3.56 and 2.05 g respectively and lignin content reduced to 17.63, 17.42, 16.90 and 17.25 g from 18.76 g, respectively. When dilute acid steam explosion pretreated corn stalk was treated by 20 FPIU/g cellulose cellulase and 3 IU/g cellulose β-glucosidase for 48 h as the substrate concentration was 100g/L, the hydrolysis yield of cellulose was 75.91 % (based on corn stover). The yield of fermentable sugars was 44.93 % (based on corn stover) as the corn stover was pretreated by dilute acid steam explosion and enzymatic hydrolysis.

A molecularly-imprinted polymer(MIP) was prepared by bulk polymerization, using kaempferol as the template molecule, 4-vinyl pyridine as the functional monomer, and ethylene glycol dimethacrylate as the cross-linker in the solvent system of CHCl ₃-DMF(3∶1, volume ratio). The synthesized polymer, i.e.kaempferol-MIP, was analyzed by FT-IR and scanning electron microscopey(SEM). In addition, the adsorption properties and mechanism were studied as well. The experimental results showed that the kaempferol-MIP exhibited a favorable affinity for kaempferol. The Scatchard analysis reveals that there exists a class of homogenous recognition sites in kaempferol-MIP within the range of certain concentration. Apparent maximal combination amount ( Q _max) and equilibrium dissociation constant ( K _D) were calculated to be 3 938 μg/g and 9.074 mg/L, respectively. The result of selectivity study showed that the selectivity coefficient is 3.24 with respect to kaempferol-rutin system.

In order to determine the technological conditions of refining wood vinegar used as acidic additives in beverage, feed of domestic animals and drinking water, the change of organic components in the refining process of wood vinegar was investigated with GC/MS in detail.Phenol, which is harmful to human body and domestic animals, was analytically traced as representative component.Result showed that through combinated application of several simple and typical refining unit operations, phenol and other hard removable harmful components could be removed.Through refining process, phenol concentration was reduced to undetectable degree from 836 mg/L of the original wood vinegar.