Table of Content

28 February 2013, Volume 33 Issue 1 Previous Issue    Next Issue

1研究报告

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Preparation and Characterization of Graft Modified Poplar Wood Powder by 4,4'-Methylenebis (Phenyl Isocyanate)/γ-Aminopropyltriethoxysilane CHEN Jian;KONG Zhen-wu;JIAO Jian;FANG Gui-gan;WU Guo-min;HUO Shu-ping 2013, 33 (1):  1-7.  doi: 10.3969/j.issn.0253-2417.2013.01.001 Abstract ( 1144 )   PDF (1688KB) ( 1304 )   Save The poplar wood powder (PWP) was surface modified by 4,4'-methylenebis (phenyl isocyanate)/γ-aminopropyltriethoxysilane(MDI/KH550) in N,N-dimethylformamide (DMF). The effects of material ratio, reaction temperature, reaction time and dosage of catalyst on the reaction were investigated. The chemical structure and surface properties of modified poplar wood powder were characterized by contact angle measurement, elemental analysis, FT-IR, 13C CP-MAS NMR and SEM. The results showed that the weight percent gain (W) of MDI/KH550 modified PWP increased by 40-60% when MDI reacted with PWP in molar ratio of 2:1 at 120 ℃ for 2 h, and the catalyst had no appreciable effect on the modification reaction. The hydrophobicity of modified PWP was improved. Related Articles | Metrics

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Preparation of Polyelectrolyte Complex by Trimethyl-lignin Quaternary Amine Salt and Carboxymethyl Cellulose WANG Chun-hai;ZANG Jian;BEI Ying;REN Shi-xue;MA Yan-li;FANG Gui-zhen 2013, 33 (1):  8-12.  doi: 10.3969/j.issn.0253-2417.2013.01.002 Abstract ( 975 )   PDF (1298KB) ( 879 )   Save Polyelectrolyte complexes were prepared by electrostatic adsorption using trimethyl-lignin quaternary amine salt and carboxymethyl cellulose based on static action theory in present study, and their structures were characterized as well. At first, quaternary amine monomer (trimethyl-epoxy propyl-amine chloride) was prepared from trimethylamine and epichrolohydrin, and then the trimethyl lignin quaternary ammonium salt was synthesized by graft reaction of this cationic monomer and purified alkali lignin. Trimethyl lignin quaternary ammonium salt-carboxymethyl cellulose(TLQAS-CMC)polyelectrolyte complex was obtained under different pH value and material ratio, and the nitrogen content of this complex was 2.11%. Polyelectrolyte complexes were characterized by FT-IR, DSC and XRD repectively. The results showed that the polyelectrolyte complex had been synthesilzed. The higher thermal decomposition temperature of the polyelectrolyte complex indicated its better thermal stability XRD revealed the complex's disordered structure, and the complex had certain water-swelling property. Related Articles | Metrics

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The Changes of Three-components in Eucalyptus during Processes of Pre-processing in High Temperature Liquid Water and Enzymolysis XU Shao-hua;WU Shu-bin;WEI Wei-qi 2013, 33 (1):  21-26.  doi: 10.3969/j.issn.0253-2417.2013.01.004 Abstract ( 961 )   PDF (1311KB) ( 1337 )   Save The physical and chemical properties of pretreatment residue were characterized with FT-IR, SEM, XRD, et al. The pre-processing process of eucalyptus in high temperature liquid water was optimized. The results show that the optimal pretreatment conditions are as follows: temperature 180 ℃,time 20 min,liquid solid ratio 20:1. The conversion ratio of xylose is 81.93% under above optimal conditions. After optimal pretreatment, most of hemicellulose were hydrolysed, but there was a little change in the crystalline degree of cellulose. Scanning electron microscope images showed that the hot water pretreatment caused the relaxation of carbohydrate of eucalyptus,the breakage of pit membrane and the increase of fiber fragments. These significantly improved the accessibility of cellulase. It was also found that further enzymolysis of optimized pre-treatment residue improved glucose conversion to 80.52%, which increased by 3.63 times compared with these raw residue. The further analysis of enzymatic residue indicated that 87.12% acid insoluble lignin from eucalyptus could be recycled, which was conducive to the comprehensive utilization of lignin. Related Articles | Metrics

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Purity, Thermal Decomposition Kinetics and Shelflife of Artemisinin by Thermal Analysis XIAO Zhuo-bing;GUO Man-man;GUO Rui-ke;TIAN Chun-lian 2013, 33 (1):  13-20.  doi: 10.3969/j.issn.0253-2417.2013.01.003 Abstract ( 968 )   PDF (1196KB) ( 983 )   Save Thermal decomposing curves of artemisinin at different heating rates were obtained under dynamic conditions in nitrogen atmosphere using non-isothermal differential scanning calorimetry analysis (DSC). Three thermal analysis kinetic methods (Kissinger, Flynn-Wall-Ozawa and Phadnis) were used to speculate the probable mechanism of thermal decomposing reaction and the kinetic parameters while the purity and melting point of artemisinin were obtained by Van'Hoff equation. The shelflife of artemisinin at room temperature was calculated by the kinetic parameters of the thermal decomposition. With the increasing of the heating rate, thermal analysis tempreture of artemisinin rose. The most probable kinetic mechanism of the thermal decomposition was two-dimensional diffusion which corresponded to Jander Equation. In accordance with the datas of atomic charges and bond orders by Gaussian simulation, the mechanism was identical with that artemisinin was decomposed into three compounds at 190 ℃. The shelflife of artemisinin at room temperature was about 3 years based on the apparent activation energy (Ea) and pre-exponential factor (A) of the thermal decomposition. Related Articles | Metrics

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Comparison of the Molecular Structure between Phenol-formaldehyde and Phenol-urea-formaldehyde Resins DU Guan-ben;LEI Hong;PIZZI Antonio 2013, 33 (1):  27-31.  doi: 10.3969/j.issn.0253-2417.2013.01.005 Abstract ( 949 )   PDF (1073KB) ( 923 )   Save To confirm the existence of co-condensation in phenol-urea-formaldehyde (PUF) resin and understand the development of molecular structure during the polymerization, the characteristic structure of PUF was analyzed based on the study of structure of phenol-formaldehyde (PF) resin with the help of two main instruments 13C NMR and MALDI-TOF-MS. Analysis results confirmed that the co-condensation among phenol, urea and formaldehyde indeed occurred and formed at the beginning of polymerization. The results also confirmed that structure of copolymer mainly derived from PF resin. Related Articles | Metrics

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Optimization of Preparation Technology of Alkali Pretreated Reed Pulp Nano-cellulose XIE Cheng;LIU Zhi-ming;WU Peng;FANG Gui-zhen;ZHAO Xu 2013, 33 (1):  32-36.  doi: 10.3969/j.issn.0253-2417.2013.01.006 Abstract ( 979 )   PDF (1025KB) ( 1558 )   Save Nano-cellulose was prepared by the hydrolysis of the alkali pretreated reed pulp with 55% (by mass fraction) sulfuric acid. The effects of reaction time, reaction temperature and alkali treatment time on yield and the mean size of nano-cellulose were studied. The optimizing preparation condition for single factor test is 1.0 h of alkali treatment time, 60 ℃ of reaction temperature and 2.0 h of reaction time. Under this condition, the yield of nano-cellulose is 54.50% and its mean size is 156.9 nm. The analysis of fourier transform infrared(FT-IR) and X-ray diffraction(XRD) showed that nano-cellulose from reed pulp pretreated by alkali is cellulose typeⅡ. The orthogonal test was investigated based on single factor test. The results showed that the technological parameters optimized by conducting orthogonal test for the yield of nano-cellulose are 1.0 h of alkali treatment time, 60 ℃ of reaction temperature and 3.0 h of reaction time. The highest yield of nano-cellulose (55.64%) with 166.3 nm mean size was achieved. Related Articles | Metrics

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Synthesis and Biological Activities of Pinic Acid-based Dithioureas MA Chang-hua;CEN Bo;DUAN Wen-gui;MA Xian-li;QIN Shan;LIN Gui-shan;QIN Yu-ning 2013, 33 (1):  37-44.  doi: 10.3969/j.issn.0253-2417.2013.01.007 Abstract ( 1005 )   PDF (1146KB) ( 924 )   Save Pinic acid was prepared via oxidation and bromoform reactions using α-pinene as starting material, and it was converted into dichloride. Then, pinic acid-based diisothiocyanates were synthesized by the reaction of pinic acid dichloride with potassim thiocyanate, followed by N-acylation reaction of pinic acid-based diisothiocyanates with phenyl amines to afford twelve novel pinic acid-based dithioureas. The structures of the target compounds were characterized by means of IR, 1H NMR, 13C NMR, MS, and elemental analysis. The preliminary bioassay showed that the title compounds exhibited a certain herbicidal activity, fungicidal activity, and plant growth regulating activity. Compound E1 displayed growth inhibition activity of 66.6% against root of rape (Brassica campestris) at the concentration of 100 mg/L (B-class activity level). Related Articles | Metrics

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Anti-lipid Peroxidation of Chlorophyll-carotene Paste from Pine Needles in Vitro ZHENG Guang-yao;HE Ling;BO Cai-ying;XIE Heng;LIU Dong-qin;LI Ruo-da 2013, 33 (1):  45-48.  doi: 10.3969/j.issn.0253-2417.2013.01.008 Abstract ( 787 )   PDF (1022KB) ( 648 )   Save Anti-lipid peroxidation of chlorophyll-carotene paste from pine needles was investigated in vitro. MDA production in rat liver homogenate and mitochondria spontaneously formed or induced by VC-Fe2+ system were measured. The results showed that the chlorophyll-carotene paste could inhibit the generation of MDA in liver homogenate under spontaneous and induced conditions, as well as in liver mitochondria under induced condition, with IC50 of 398.72 mg/L, 355.05 mg/L and 687.02 mg/L, respectively. The swelling of liver mitochondria was also inhibited with dose-response relationship. It is suggested that chlorophyll-carotene paste from pine needles possessed anti-oxidant activity in vitro. Related Articles | Metrics

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Preparation of the Films Based on Bayberry Tannin and Its Adsorption of Zn2+ and Cu2+ LUO Yang;LU Ai-xia;LUO Xue-gang 2013, 33 (1):  49-53.  doi: 10.3969/j.issn.0253-2417.2013.01.009 Abstract ( 908 )   PDF (1089KB) ( 791 )   Save A kind of crosslinked films based on bayberry tannin/konjac glucomannan(KGM)/rice flour was prepared and analyzed by FT-IR. The adsorption behaviors of the films to copper and zinc ions were investigated. The results indicated that the adsorption capacity for Cu2+ and Zn2+ reached a maximum value of 12.79 mg/g and 6.13 mg/g respectively at 25℃ and pH 5.0 when the initial concentration of Cu2+ and Zn2+ was 200 mg/L. The adsorption capacity was un-significantly increased with the rise of temperature. The adsorption isotherms of copper and zinc ions could be well described by the Freundlich equation. The adsorption kinetics data could be fitted by the pseudo-second-order model. In addition, crosslinked films based on bayberry tannin can selectively remove Cu2+ from the mixture solution of Cu2+ and Zn2+. Related Articles | Metrics

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Flooding Extraction of Betulin LUO Sha-man;DING Wei-min;YU Tao;WANG Yang 2013, 33 (1):  54-58.  doi: 10.3969/j.issn.0253-2417.2013.01.010 Abstract ( 891 )   PDF (1240KB) ( 836 )   Save Betulin was extracted from white birch bark using a modified flooding extraction equipment. The equipment was easy-connected and conveniently-operated. It effectively improved the product purity without the change of yield. The effects of ethanol concentration and extraction time on the yield and purity of betulin was investigated. The optimal conditions were 70% of ethanol concentration, 50 min of extraction time. Under above conditions, the yield of betulin reached (88.39±1.77)% and the product purity was (80.34±0.25)%. Related Articles | Metrics

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Syntheses of Tert-butyl Esters of 3,28-Di-o-succinyl-aminoacyl-betulin and Their Effects on the Proliferation and Apoptosis of HepG-2 SUI Ruo-jun;ZHANG Xiao-li;FANG Gui-zhen;FU Yu-jie 2013, 33 (1):  59-63.  doi: 10.3969/j.issn.0253-2417.2013.01.011 Abstract ( 789 )   PDF (1174KB) ( 1031 )   Save Tert-butyl ester of 3,28-di-o-succinyl-alanylamino-betulin(SAB) and tert-butyl ester of 3,28-di-o-succinyl-glycylamino-betulin(SGB) were synthesized from betulin. The structure of the products were identified by 1H NMR and MS. In order to study the effects of betulin derivatives on inhibiting tumor cells, using MTT assay, the SAB and SGB carried out on the HepG-2(Human Hepatocellular carcinoma cells) activity analysis. The results showed that SAB and SGB were specific to promote their apoptosis of HepG-2. The apoptosis was time and concentration dependent. Effect after 72 h ,IC50 were 15.33 μmol/L and 17.01 μmol/L. IC50 of SAB and SGB was significantly lower than IC50 of betulin. On HepG-2 cells, the IC50 of SAB is about 1/6 of the IC50 of betulin; The IC50 of SGB is about 1/5 of the IC50 of betulin. The results showed that SAB and SGB's anti-tumor activity was better than betulin. Related Articles | Metrics

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The Deactivation Mechanism and Regeneration Methods of Resin Catalyst for the Esterification of Camphene QU Yan-yan;ZHENG Hui-dong;ZOU Wen-hu;WANG Ying-shu;WU Yan-xiang 2013, 33 (1):  69-73.  doi: 10.3969/j.issn.0253-2417.2013.01.013 Abstract ( 1067 )   PDF (1995KB) ( 1202 )   Save Cation exchange resin as catalyst for the preparation of isobornyl acetate by the esterification of camphene is widely used in industry. However this type of catalyst is easy to lose its activity during the reaction. In this paper, the deactivation mechanism and regeneration method of the catalyst (CT800) were investigated. It showed that the deactivation of catalyst was mainly caused by the overlay of polymer byproducts on the resin activity centers. The deactivated catalyst can be regenerated by washing with ethanol. The activity of the regenerated catalyst is close to that of fresh one. This proves that this generation method is effective. Related Articles | Metrics

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Comparison of Immobilized Xylanase Using Mesoporous Titania and Sodium Alginate as Carriers SHAN Zong-xing;WEI Ce;LI Xin;SONG Xiang-yang;ZHANG Yang;ZHANG Wei-wei;XU Chen-chen 2013, 33 (1):  64-68.  doi: 10.3969/j.issn.0253-2417.2013.01.012 Abstract ( 948 )   PDF (1758KB) ( 845 )   Save In order to improve the stability of xylanase in biocatalysi, mesoporous titania (M-TiO2) and sodium alginate were used to immobilize xylanase. The properties of immobilized xylanase were also investigated in this study. The optimum immobilization by mesoporous titiania was obtained as 20.36 IU/g xylanase was immobilized at 65 ℃ for 0.5 h under pH 6.0. The maximal immobilized rate is 93.37%.In contrast, the xylanase was immobilized when 35 g/L sodium alginate was selected to handle the enzyme at 65 ℃ for 2 h under pH 6.0 environment in the presence of 20 g/L CaCl2. Maximal immobilized rate is 28.77%.In regard of the free and immobilized xylanase on sodium alginate, immobilized xylanase by mesoporous titania has better thermostability and pH stability. Related Articles | Metrics

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Adsorption Properties of Ligustrazine Hydrochloride onto Rosin-based Macroporous Adsorption Resin LI Peng-fei;MU Cai-hua;LEI Fu-hou;LU Jian-fang;LIU Zu-guang; 2013, 33 (1):  74-80.  doi: 10.3969/j.issn.0253-2417.2013.01.014 Abstract ( 813 )   PDF (2224KB) ( 893 )   Save The adsorption kinetics and thermodynamics of the rosin-based macroporous adsorption resin which was used as an adsorbent for ligustrazine hydrochloride was studied through static adsorption experiments. The results showed that the adsorption rates of ligustrazine hydrochloride onto rosin-based macroporous adsorption resin gradually reduced with time, and followed the second-order kinetics, and basically reached equilibrium after 3.5 hours. The adsorption process belongs to slow adsorption, and it is effected by film diffusion and intra-particle diffusion. The equilibrium for the adsorption of ligustrazine hydrochloride from aqueous solutions by rosin-based macroporous adsorption resin could be fitted to Langmuir-type of isotherm equation and maximum sorption was about 144.5 mg/g when the optimum temperature was 313 K. The studies on the thermodynamics showed that the adsorption ΔH>0, ΔS>0, the change in free energy of adsorption decreases from positive to negative with the increment of temperature, the adsorption process is a spontaneous and endothermic one. Related Articles | Metrics

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Synthesis and Characterization of Dehydroabietic Acid-alcohol Spice Esters QIN Shan;DUAN Wen-gui;LAI Gang;WU Guang-sui;MA Chang-hua 2013, 33 (1):  81-84.  doi: 10.3969/j.issn.0253-2417.2013.01.015 Abstract ( 827 )   PDF (1051KB) ( 794 )   Save Four types of dehydroabietic acid-alcohol spice esters, i.e. dehydroabietic acid-cinamic alcohol ester, dehydroabietic acid-fenchol ester, dehydroabietic acid-borneol ester and dehydroabietic acid-menthol ester, were synthesized by O-acylation reaction of dehydroabietyl acyl chloride and alcohol spices using dehydroabietic acid as starting material. Suitable synthetic conditions of the target products were found to be as follows: reaction time 10 h, reaction temperature 95-100 ℃, molar ratio of reactants 1.2:1 (alcohol:chloride). The target products were characterized by means of IR, UV, NMR, and MS. Related Articles | Metrics

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Sulphonation of Black Liquor and Properties of the Modified Product JI Dong;MA Hong-ming;HE Ming;SHI Yi-jun;LUO Zhen-yang 2013, 33 (1):  85-90.  doi: 10.3969/j.issn.0253-2417.2013.01.016 Abstract ( 924 )   PDF (1192KB) ( 841 )   Save The lignin in black liquor was firstly extracted first and then sulphonated in present study. The optimum sulphonation conditions were determined by staged orthogonal design (hydroxymethylation first, then sulphonation). The sulphonated lignin (SL) was prepared. The product was characterized by FT-IR. Its sulphonation degree, paste fluidity, and weight-average molecular weight were measured respectively. After 4 g acid precipitated lignin in 40 g water reacted with 5 g formaldehyde at 75 ℃ for 2 h under the pH 11 environment, the pH of the system was adjusted to 7 and then 2 g sodium sulphite was added and reacted for another 4 h at 140 ℃. The optimal sulfonated lignin was obtained. FT-IR spectra showed that considerable sulphonic groups were introduced onto sulphonated lignin. The sulphonation degree and molecular weight of sulphonated lignin are 0.821 mmol/g and 22.97 ku, respectively. It was better than that of commercially available sodium lignosulphonate (SLS). This indicated that the paste fluidity of sulphonated lignin was also greater at water-cement ratio of 0.35 and identical dosage. Related Articles | Metrics

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Seasonal Variation of Antioxidant Components of Geranium sibiricum Linne YANG Yu-chun;LI Ji;FU Yu-jie;ZU Yuan-gang; 2013, 33 (1):  91-96.  doi: 10.3969/j.issn.0253-2417.2013.01.017 Abstract ( 967 )   PDF (1043KB) ( 1096 )   Save Seasonal variation of corilagin, geraniin as well as total phenolic contents during the growth period, where were obtained from the whole grass, roots, stems and leaves of Geranium sibiricum Linne, were investigated. The antioxidant capacity (FRAP, DPPH, ABTS) were evaluated to confirm the optimum picking part and stage. Results showed that corilagin (CG), geraniin (GE) and total phenolic contents (TPC) increased dramatically in the vegetative growth period (137.567 3±1.890 7 GAE/g extracts)and decreased gradually during the reproductive growth and withering period (74.7518±1.010 1 GAE/g extracts), while the total flavonoid contents (TF) was high in summer and reached the highest during the withering period. The optimum picking part and time were the whole grass and July, respectively, and the total antioxidant capacity (FRAP) was (12.321 5±0.188 2)mmol/g extracts. Correlation of the data obtained from principal composition analysis (PCA) showed that TPC, CG and GE had high relationship (r>0.753) and they also had high relationship with antioxidant capacity (|r|>0.75). Related Articles | Metrics

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Chemical Components of Fallen Leaves of Salix matsudana Koidz ZHANG Shu-jun;CHANG Hai-bo;LV Wei-qiang;DING Yu;LI Ya-fu 2013, 33 (1):  97-101.  doi: 10.3969/j.issn.0253-2417.2013.01.018 Abstract ( 857 )   PDF (1054KB) ( 993 )   Save The chemical components of the fallen leaves of Salix matsudana were studied. Thirteen compounds were isolated and identified as salicylol(1), salicin(2), salicin-2'-benzoate(3), caffeic acid(4), luteolin-7-O-β-D-glucoside(5), chrysoerid(6), catechol(7), loliolide(8), chlorogenic acid(9), protocatechuic acid(10), 3,5-dimethoxy-4-hydroxyphenethyl alcohol(11), succinic acid(12). butyric acid(13). The compounds 6,8,10-13 were isolated from Salix matsudana Koidz for the first time, and the mass fraction of salicin was over 0.07%. Related Articles | Metrics

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Determination of Mineral Elements of Scindapsus aureus (Linden ex Andre) Engler WEI Yong-sheng;ZHENG Min-yan;GENG Wei;GU Yuan-zi 2013, 33 (1):  102-106.  doi: 10.3969/j.issn.0253-2417.2013.01.019 Abstract ( 765 )   PDF (1107KB) ( 1004 )   Save By wet digestion procedure with HNO3/H2O2 to prepare samples, the mineral elements in Scindapsus aureus (Linden ex Andre) Engler were completely analyzed by inductively coupled plasma optical emission spectrometer (ICP-OES). The results showed that there were 16 mineral elements including K, P, Ca, Mg, S, Al, Si, Fe, Na, Mn, Zn, Sr, B, Ba, Cu and Ti in Scindapsus aureus. Except that of Ti, the RSD of other mineral elements were less than 4%. The recovery rate was between 90.3% and 110.9%. Among them, those of 10 mineral elements were within (100±5)%. The mass fraction of such necessary macrometals in the human body, such as K, P, Ca, Mg, S and Na, were 0.66%, 0.40%, 0.27%, 0.23%, 0.17% and 0.004%, respectively. The contents of microminerals such as Fe, Zn, Cu, Si, Mn, and B, were 48.4, 30.8, 6.5, 151, 36.3 and 16.5 mg/kg, respectively. Related Articles | Metrics

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Extraction of Polyphenols from Leaves of Rhus chinensis Mill and Its Antioxidant Activity QIU Zhen-yu;TANG Ming-li;ZHANG Xuan;ZHANG Ping-ping;WU Li-fang 2013, 33 (1):  107-112.  doi: 10.3969/j.issn.0253-2417.2013.01.020 Abstract ( 1012 )   PDF (1074KB) ( 1404 )   Save Three extraction methods (heat, ultrasound-assisted, microwave-assisted) and four different solvents (water, ethanol, methanol and acetone) were used to compare the extraction efficiencies of polyphenols from leaf tissues of Rhus chinensis Mill. The thermal extraction and ethanol solvent was chosen as the basic method for further study. By using single factor test and orthogonal test, the optimized extraction conditions were as follows: solid-liquid ratio 1:15, extraction time 60 min, extraction temperature 60 ℃ and 60% ethanol solvent. Crude polyphenol powder was obtained from solution of direct centrifugation, ethyl acetate phase or water part after ethyl acetate extraction. The polyphenol purity was determined as 37.53% by direct dry, 19.26% in water phase, 59.44% in ethyl acetate phase. Their radical-scavenging activities in vitro were slight lower than to commercial tea polyphenol with 98% purity, but more than that of Vc. Related Articles | Metrics

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Chemical Components of Chinese Herbal Formula of Gankang ZHANG Jin-cai;CHEN Guang-ying;HAN Chang-ri;CHEN Wen-hao;ZHAN Xue-mei;ZHANG Wei 2013, 33 (1):  113-116.  doi: 10.3969/j.issn.0253-2417.2013.01.021 Abstract ( 1023 )   PDF (995KB) ( 936 )   Save To study the hypoglycemic mechanism from Chinese Herbal Formula of Gankang, the chemical constituents of its active fraction were investigated in this study. Thirteen compounds were isolated and purified by silica gel column, Sephadex LH-20, and C18 chromatography, respectively. Their chemical structures were elucidated on the basis of physicochemical properties and spectral data. Thirteen compounds were identified as: (Z)-resveratrol(3,5,4'-trihydroxystilbene)-3-O-β-glucoside(1), kaemperol-3-O-β-D-glucopyranoside(2), kaemperol(3), 4'-dihydrophaseic acid(4), leptosphaepin(5), arbutin(6), 4-caffeoyl quinic acid(7), 3,4-dimethoxyphenyl-ethyl-ether(8), 2-butylated-4-hydroxyanisole(9), α-tocoquinone(10), isovanillic acid(11), mannitol(12) and β-daucosterol(13). They are all firstly isolated from this traditional Chinese medicine Related Articles | Metrics

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Simultaneous Determination of Five Flavonoids in Pteris multifida Poir. by HPLC LIU Jian-qun;REN Xiao-jing;SHU Ji-cheng;ZHANG Rui;ZHANG Wei;PAN Jing-hang 2013, 33 (1):  117-120.  doi: 10.3969/j.issn.0253-2417.2013.01.022 Abstract ( 997 )   PDF (1053KB) ( 1070 )   Save An HPLC method was established to the determination of five flavonoids (vicenin-2, lonicerin, rhoifolin, luteolin and apigenin) in Pteris multifida. Separation was carried out on a Diamonsil C18 column (4.6 mm×250 mm, 5 μm). The mobile phase was acetonitrile-water (containing 0.1% formic acid) with gradient elution (0-10 min, 15:85; 20-30 min, 25:75; 35-40 min, 50:50). The flow rate was 1.0 mL/min at 25 ℃, and the detection wavelength was set at 350 nm. The average recoveries for vicenin-2, lonicerin, rhoifolin, luteolin and apigenin were 97.18%-105.20% (RSD1.72%-3.20%), 95.02%-104.92% (RSD 1.96%-2.93%), 98.45%-103.70% (RSD 1.66%-2.70%), 96.41%-104.73% (RSD 1.03%-3.46%), 95.82%-99.34% (RSD 1.85%-3.95%), respectively. These compounds showed good linearity(r≥0.999, n=5) in the detected concentration ranges. There were no significant differences of mass factions of the five flavonoids from different regions. The total mass factions of the five flavonoids were in the range of 0.5-2.2 mg/g. Related Articles | Metrics

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Chemical Components of Alpinia sichuanensis Z.Y.Zhu WU Qiu-yan;WANG Ping-ping;WANG Kui-wu;JIN Zhi-min 2013, 33 (1):  121-124.  doi: 10.3969/j.issn.0253-2417.2013.01.023 Abstract ( 769 )   PDF (1048KB) ( 952 )   Save Chemical constituents of Alpinia sichuanensi were studied.Eight compounds were isolated and purified by Silic Gel and Sephadex LH-20 column chromatography. Their structures were elucidated by chemical and spectroscopic methods as 3, 5-dihydroxy-4',7-dimethoxyflavone(1), 4',7-dihydroxy-5-methoxyflavanone(2), 4',5,7-trihydroxyflavone(3), 5,7-dihydroxy-4'-methoxyflavone (4), p-coumaric acid(5), β-daucosterol(6), β-sitosterol(7), stigmasterol(8).Compounds 1-5 are isolated from this plant for the first time. Related Articles | Metrics

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Chemical Components of the Hevea brasiliensis Skim TANG Li-chang;WANG Ning;YAO Hai-ping;YANG Xian-hui;DENG Shi-ming;ZHANG Chao-yang 2013, 33 (1):  125-129.  doi: 10.3969/j.issn.0253-2417.2013.01.024 Abstract ( 994 )   PDF (1028KB) ( 982 )   Save The chemical constituents of the Hevea brasiliensis skim were studied. Compounds were isolated and purified by silica gel column chromatography and Sephadex LH-20. Their structures were identified by physicochemical properties and spectral analyses. Thirteen compounds were obtained and the twelve ones of them were identified : Friedelin (Ⅰ), Lupeol (Ⅱ), β-Sitosterol (Ⅲ), Hexadecanoic acid (Ⅳ), Heptadecanoic acid (Ⅴ), Daucosterol (Ⅵ), Nicotine acid (Ⅶ), Succinic acid (Ⅷ), Gallac acid (Ⅸ), Fructose (Ⅹ), Quebrachitol (Ⅺ), 6-hydroxy-cleistopholine (Ⅻ). Compounds Ⅰ, Ⅱ, Ⅵ, Ⅶ Ⅸ and Ⅻ were isolated from Hevea brasiliensis skim for the first time. Related Articles | Metrics

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Research Progress of Phenolic Compounds from Wanlnut(Juglans regia L.) CHEN Hong-xia;WANG Cheng-zhang;ZHOU Hao;YE Jian-zhong 2013, 33 (1):  130-134.  doi: 10.3969/j.issn.0253-2417.2013.01.025 Abstract ( 824 )   PDF (1019KB) ( 1581 )   Save Polyphenols are widely distributed in various tissues of walnut, which is the one of the secondary product of fruit trees and fruit, with antioxidation and delays the body's failure effect. This paper reviews the research progress of extraction, purification, quantitative analysis, composition and biological activity of walnut tissues, in order to provide a reference for comprehensive study of walnut phenolic compounds and promote the efficient development of the walnut polyphenol. Related Articles | Metrics