Table of Content

30 August 2010, Volume 30 Issue 4 Previous Issue    Next Issue

研究报告

Select

Lipid-soluble Components from Fig Residue and Its Anti-tumor Activities in vitro WANG Zhen-bin;MA Hai-le;MA Xiao-ke;GAO Zong-ying;CHANG Jin-ke 2010, 30 (4):  48-52.  Abstract ( 705 )   PDF (972KB) ( 717 )   Save Extraction and purification technology of lipid-soluble anti-tumor active components from fig residues were studied. Thirteen crystals were got after column chromatography, spectrometric study and structural analysis. The anti-tumor activity of these 13 crystals was investigated on blood tumor cell line(U937), lung tumor cell line(95D)and stomach tumor cell line(AGS)in vitro. The Number 6 crystal was identified as β-sitosterol and validated as anti-tumor component for its fifty percent inhibition content (IC50)lower than 10mg/L. The optimum lipid-soluble anti-tumor components extraction condition are as follows, using petroleum ether(boiling point from 60 to 90℃) as solvent and the fig residues granularity 750μm, extracting temperature 85℃, extracting time 130min, the ratio of solid to solvent 1:11(g:mL), extracting twice. As a result the anti-tumor substance extraction yield is 23.1g/kg. The anti-tumor component was identified as β-sitosterol after column chromatography, spectrometric study and structural analysis. Related Articles | Metrics

Select

Study on the Active Component and Antioxidant Activities of Extract from Pinus koraiensis Sieb.et Zucc.Seed Shell SU Xiao-yu;WANG Zhen-yu; 2010, 30 (4):  99-102.  Abstract ( 750 )   PDF (718KB) ( 791 )   Save Study on the bioactive component and antioxidant activities of extract from Pinus koraiensis Sieb.et Zucc.(PKS) seed shell, was carried out. Folin-Ciocalteau and AlCl3 methods were adopted for the analysis of polyphenol and flavonoid extracts. Radical scavenging assay, lipoprotein oxidation inhibition assay and metal ion chelation power test were adopted to detect the antioxidant activity of extract. Result showed that the contents of polyphenol and flavonoid in the extract were 262 and 174mg/g respectively. The extract has highly effective inhibition for lipoprotein oxidation, at 0.5 to 1.5g/L, the inhibitory rate was increased from 49.27% to 95.44%; the extract also has strong radical scavenging effect, the inhibitory rate for 2,2'-azido-bis(3-ethylbenzthiazoline-6-sulfonic acid)(ABTS) radical was increased from 17.53% to 97.83% at 0.16 to 1.6g/L respectively; the extract has effect on Fe2+ chelation and the chelation rate was 57.74% at 10g/L. Related Articles | Metrics

Select

Progress for Catalytic Selective Hydrogenation of Citral YANG Yong-jun;YIN Du-lin;MAO Li-qiu;ZUO Chuan-peng;LIU Xian-xiang;GONG Yan 2010, 30 (4):  113-118.  Abstract ( 995 )   PDF (852KB) ( 744 )   Save Citral was used widely since its hydrogenation products were applied in the area of perfume and medicine intermediate production extensively. In this paper, the development of the catalysts for catalytic hydrogenation of citral in the latest five years were summarized, especially in the preparation of catalysts and influences of carrier and active agent on the catalytic performance of citral selective hydrogenation. The selective hydrogenation of citral into various products including geraniol plus nerol, citronellal, dihydrocitronellal, citronellol and 3,7-dimethyloctanol were discussed, which will be useful for further study on selective hydrogenation of citral. Related Articles | Metrics

综述评论

Select

Research Progress on Production of Phenolic Resins from Products of Biomass Pyrolysis & Liquefaction FENG Hao;ZHENG Zhi-feng;JIANG Jian-chun;HUANG Yuan-bo 2010, 30 (4):  107-112.  Abstract ( 630 )   PDF (1052KB) ( 806 )   Save The utilization of liquids from pyrolysis and liquefaction of biomass and related materials for substituting synthetic phenolic compounds in phenolic resins are reviewed. Many biomass feedstocks could be used as sources of pyrolysis and liquefaction for production of pyrolysis liquids enriched in phenolics, such as hardwood and softwood, bark, residual lignins and agricultural wastes. Resins have been prepared utilizing either the whole liquid product, or a phenolics-enriched fraction obtained after further processing, such as fast-pyrolysis oil, vacuum-pyrolysis oil, high-pressure pyrolysis oil, liquefied products from biomass in phenol and phenolysized lignin. These resins were primarily employed in the manufacture of wood panels and their properties were also discussed. The problems of pyrolysis liquids for substituting synthetic phenolic compounds in the resins and the development trend in the future were pointed out. Related Articles | Metrics

研究报告

Select

Progress in Steam-explosion Pretreatment Technologies and Its Effect on Bioconversion of Cellulosic Ethanol WANG Xin 2010, 30 (4):  119-125.  Abstract ( 754 )   PDF (1171KB) ( 1061 )   Save Lignocellulosic biomass is considered as one of the most promising feedstock for producing fuel ethanol. However, the structure and crystallinity of lignocellulose hamper the accessibility of cellulose enzyme, so that a suitable pretreatment process is necessary. Recently, steam explosion, because of its low cost, low energy consumption and pollution-free has been received substantial attention. The influences of parameters in pretreatment by methods of steam explosion,such as:technologies of dilute acid steam explosion(DASE),dilute base steam explosion(DBSE),ammonia fiber explosion(AFEX)and compound steam explosion are described, and several steam-explosion pretreatments and equipments are discussed. Meanwhile the applied research of pretreatment for bioconversion to ethanol is also reviewed. Related Articles | Metrics

Select

Refinement of 2,3,4,4'-Tetrahydroxybenzophenone WANG Guang-hui;ZHANG Zong-he;ZHONG Chong-mao;DU Yun-ping 2010, 30 (4):  33-37.  Abstract ( 682 )   PDF (918KB) ( 648 )   Save After discoloration, crude 2,3,4,4'-tetrahydroxybenzophenone, was adsorbed and separated by chromatographic column and micro-porous membrane, the impacts of membrane aperture and operating pressure on separation effect were studied. Furthermore, the adsorption-desorption regenerating test of the used column was done, the impacts of temperature and the amount of eluate on elution results were investigated. The results showed that the optimum separation conditions were: membrane aperture 0.2μm, pressure 0.06MPa, working the column in series with micro-porous membrane. Under these conditions the indices of the product were as follows: mass fraction ≥99.5%, impurities of Ca2+, Fe3+, Na+≤200μg/L. Using 300mL 50% ethanol as eluant for the regeneration of the used column, the desired results were obtained. Related Articles | Metrics

Select

Synthesis of Terpineol Catalyzed by Acid-doped Nano-polyaniline YANG Xiao-xia;CHEN Si-wu;WANG Ya-ming;JIA Qing-ming 2010, 30 (4):  89-92.  Abstract ( 657 )   PDF (1050KB) ( 735 )   Save Polyaniline nanofibers doped by a variety of acids(hydrochloric acid, sulfuric acid, chloroacetic acid, etc.) were prepared using rapid mixing method. Result of scanning electron microscopy showed that the diameter of the polyaniline fiber is 50-200nm. The hydration of turpentine was regarded as the goal reaction to study the effects of preparation conditions on the catalytic activity. The results showed that the optimum conditions were as follows: sulfuric acid as doping acid, molar concentration 2.0mol/L, aniline molar concentration 0.3mol/L, molar concentration ratio of ammonium persulfate and aniline 1:1, under these conditions conversion rate of α-pinene reached 93.80%, and the max yield of α-terpineol reached 60.60%. Related Articles | Metrics

Select

Study on Extraction Technology of Berberine from Phellodendron amurense Rupr.by Supercritical CO2 ZHANG Yu-hong;WEN Hui 2010, 30 (4):  103-106.  Abstract ( 651 )   PDF (757KB) ( 734 )   Save The phloems of Phellodendron amurense Rupr. were extracted with supercritical CO2 to obtain berberine. Factors affecting extraction yield were discussed, the optimum technological conditions were validated by orthogonal experiments design as follows: extraction pressure 25MPa, temperature 50℃, extraction time 60min, and entrainer 95% ethanol. The yield of berberine was 67.56% under the above-mentioned extraction conditions. Related Articles | Metrics

Select

Determination and Content Changes of Total Flavonoids and Three Flavonoid Aglycones in Crotalaria ferruginea Grah TANG Qian;YANG Xian;LIU Ying-jie;WANG Bo-chu;ZHANG Xue 2010, 30 (4):  93-98.  Abstract ( 778 )   PDF (1185KB) ( 566 )   Save Rutin, quercetin, genistein and kaempferol were used as reference substances. Their contents were determinated in Crotalaria ferruginea Grah. Ultraviolet spectrophotometry was used to determine absorbance of rutin at 500 nm wavelength.Thus total flavonoids were inspected.The contents of quercetin, genistein and kaempferol were determined by HPLC from C.ferruginea. The results were as follows: In the growing season(from March to August), the contents of the 4 components were all low, but in the dormancy(October to the following February), the contents of the four components gradually increased. The contents in the following February was the highest. The contents in the samples from different growth years increasd with the growth period. In the plants which had grown from 1 to 6 years, the contents of the three flavonoid aglycones increased with increasing age. In the plants which had grown from 6 to 9 years, the contents had a linear ascending tendency. After the plant grew more than 9 years, the contents increased slower. In the samples from diffenert growth years, the content of flavonoids was also increased with increasing age. But the difference was that in the whole process, the content of flavonoids had a curved change. The content of 5-year old plant was the lowest(13.42 mg/g), and 10-year old plant was the highest(25.10 mg/g), the average annual increase was 1.168 mg/g. The results showed that the hightest contents of total flavonoids, quercetin, genistein and kaempferol were in C.ferruginea from Linshui Chang'an of Sichuan Province. The contents of total flavonoids and quercetin in C.ferruginea from Binjiang and Kunming of Yunnan Province had little difference and they were less than those of Chang'an. Only the contents of genistein and kaempferol in C.ferruginea from Binjiang were more than in Kunming. The lowest contents of total flavonoids, quercetin, genistein and kaempferol were in C.ferruginea from Leishan County, Guizhou Province. Related Articles | Metrics

Select

Extraction and Antioxidation Properties of Seabuckthorn Pigment ZHU Hong-mei;ZHAO Meng 2010, 30 (4):  78-84.  Abstract ( 728 )   PDF (1442KB) ( 847 )   Save Optimum pigment extraction methods were established with response surface methodology on the basis of single-factor experiment of liquid to material ratio, extracting temperature and extracting time. Using volume fraction of 95 % ethanol as solvent; the concentration of product was used to evaluate final result. The results indicated that the optimum extraction conditions were as follows: liquid to material ratio 22.4:1(mL:g), extraction temperature 63 ℃ and time 2.9 h. The research on anti-oxidation properties showed that seabuckthorn pigments had better effects on reducing Fe3+ and eliminating 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl(DPPH·)and superoxide anion free radicals(O2-·) than butylated hydroxytoluene(BHT). As to the scavenging of hydroxyl free radicals(·OH), seabuckthorn pigments had a higher effect than BHT when mass concentration was lower than 6.0mg/L; while it had lower effect than BHT when mass concentration was higher than 6.0mg/L. Related Articles | Metrics

Select

Study on Extraction of Curcuminoids by Multi-indexes Comprehensive Evaluation Method KUANG Chun-tao;LI Xiang-zhou;ZHANG Sheng;XUE Hai-peng;WU Xue 2010, 30 (4):  65-68.  Abstract ( 642 )   PDF (604KB) ( 679 )   Save There are many antioxidants in curcuminoids, such as curcumin, demethoxycurcumin and bisdemethoxycurcumin. Based on orthogonal experiments, the effects of extraction temperature, volume fraction of ethanol, time and solid-liquid ratio on yield of curcuminoids, scavenging rates of diphenyl picryl hydrazyl free radical(DPPH·) and hydroxyl free radicals(·OH) have been investigated by multi-indexes comprehensive method. The optimum conditions are as follows: extraction temperature 80 ℃, volume fraction of ethanol 90%, extraction time 1.5h and solid-liquid ratio 1:8(g:mL). Under the optimum conditions, the yield of curcuminoids is 4.37%, and the scavenging rates of DPPH· and ·OH are 8.58% and 20.67%, respectively. Related Articles | Metrics

Select

Dynamic Sorption of Cu(Ⅱ) from Waste Water by Rice Husk in Fixed Bed Column DENG Zai-fang;LUO Xue-gang;LIN Xiao-yan 2010, 30 (4):  69-72.  Abstract ( 638 )   PDF (757KB) ( 691 )   Save The column adsorption of rice husk to Cu2+ in water was studied. Putting rice husk into glass column of a diameter 3cm, and a length of 50cm, with bed depths of 9, 12 and 15cm respectively. The column was eluted with Cu2+ solution of 12 mg/L at 10 mL/min continuously. The breakthrough time(ta) and exhaust time(tb) were increased with the increase of bed length(h) under the correlation of ta=0.15h-0.467, tb=0.15h+0.639. For the column of bed depth 15cm, Cu2+solutions of 12 mg/L concentration at different flow rates were eluted respectively, the length of adsorption zone increased under the correlation of δ=0.245V+3.99, R2=0.992. Research on dynamic test showed that adsorption of Cu2+ by the rice husk could be described by Bohart-Adams model; and the material was found to be an efficient media for the removal of Cu2+ in continuous mode using fixed bed column. Adsorption capacity reached 1.96-1.20 mg/g at flow rate from 5 to 25 mL/min. Related Articles | Metrics

Select

Influence of Specific Surface Area of Super-high Specific-surface-area Activated Carbon on Storage Property of Natural Gas ZHOU Gui-lin;JIANG Yi;QIU Fa-li 2010, 30 (4):  29-32.  Abstract ( 660 )   PDF (568KB) ( 1064 )   Save Influence of specific surface area of super-high specific-surface-area activated carbon on adsorption storage of natural gas was studied under the condition of the same mesoporous distribution. The results showed that high specific-surface-area was advantageous for storage of the adsorbed natural gas. Adsorption amount of natural gas evidently depends on the pressure and temperature for activated carbon with high specific-surface-area. The adsorption amount of natural gas under low pressure(<3.5MPa) follows the exponential equation. The adsorption amount of natural gas increases with the increment of the pressure and it abides to the linear equation when pressure is higher than 3.5MPa. At 273K and 9.0MPa, natural gas desorption amount increased 25.5% when the specific surface area increased 23.7% for same pore size distribution of activated carbon, while the natural gas desorption amount reached 1 248.4mL/g when the specific surface area reached 3 348m2/g. Related Articles | Metrics

Select

Resistances of Chemically Treated Bambusa arundinacea against Fungi, Termite and Fire SADHNA Tripathi 2010, 30 (4):  7-14.  Abstract ( 718 )   PDF (512KB) ( 626 )   Save The present work deals with the protection of Bambusa arundinacea against fire, fungi and termites by treatment with different compositions of fire retardants and the eco-friendly preservative ZiBOC. Bamboo culms splits treated with 6 compositions at 15% mass fraction respectively were subjected to three tests, viz: flame penetration, flammability and rate of burning as per Indian Stan-dard. Compositions of: 1.ammonium sulphate+ammonium phosphate+ZiBOC; 2.ammonium sulphate+ZiBOC; 3.ammonium phosphate+ZiBOC; 4.magnesium phosphate+magnesium pyrophosphate + ZiBOC; 5.magnesium phosphate+ZiBOC and 6.magnesium pyrophosphate+ZiBOC at 15% mass fraction were used. Retentions of different chemical compositions, under test, in bamboo were in the range of 9-11.5kg/m3. Decay test against Trametes versicolor(white rot) exhibited that compositions 2, 3, 5 and 6 imparted significantly high protection(up to 95%;p<0.05) to bamboo blocks against white rot as compared to control. Approximately 43%-67% protection could be achieved to bamboo, treated with different compositions, over control, against Oligoporus placentus(brown rot). All specimens treated by compositions 2-6 were completely protected against termite after the 1st and the 2nd seasons of exposure except composition 4, where very slight attack of termite was seen after the 2nd season while control specimens were badly damaged. A few compositions had remarkable contribution toward protection of samples against fire. Related Articles | Metrics

Select

Study on Activated Carbon Applied to Intensive Treatment of Wastewater TAN Jun-li;CHEN Yu-ping;LU Tao-li;YANG De-qin;JIANG Jian-chun 2010, 30 (4):  38-42.  Abstract ( 583 )   PDF (943KB) ( 670 )   Save The sand filtration-activated carbon adsorption technique for pharmaceutical wastewater treatment was studied. Sand filtration ensured the water requirements of activated carbon adsorption column. Three-stage activated carbon adsorption was used. The experiment revealed that it can meet the processing requirements by using this technique, controlling the influent CODCr concentration not more than 400mg/L, the flux of 25L/h, water feed less than the times of 1000, and the effluent CODCr can be controlled within 100mg/L. Besides, two methods were used to regenerate the adsorption-saturated activated carbon. The results indicated that the treatment effect of acid-base regeneration is obviously superior to solvent regeneration method. Related Articles | Metrics

Select

Preparation and Characterization of Thermoplastic Soy Protein Isolate LU Yan;LUO Xue-gang;LIN Xiao-yan 2010, 30 (4):  15-19.  Abstract ( 589 )   PDF (1025KB) ( 838 )   Save The thermoplastic copolymers(T-SPI) were prepared from soy protein isolate(SPI) grafted with methyl acrylate(MA) and methyl methacrylate(MMA) in urea aqueous solution with ammonium persulfate as initiator and sodium sulfite as denaturant. The influences of the reaction conditions on the grafting rate were investigated. The grafting rate of 16g SPI was preferable under the conditions of urea concentration 2mol/L, initiator concentration 35mmol/L, monomer concentration 1.23mol/L, reaction temperature 70℃, reaction time 4h in 250mL deionized water. The material was analyzed by means of FT-IR, DSC and torque rheometer. The results indicated: the monomer is grafted on SPI successfully, and T-SPI has a glass transition temperature of 66.35℃, the plasticizing time is 113s. All of them proved that this material has a good thermoplasticity. Related Articles | Metrics

Select

Physico-chemical and Spectral Properties of Theabrownin from Pu-erh Tea TAN Chao;GUO Gang-jun;LI Bao-cai;ZHOU Hong-jie;GONG Jia-shun 2010, 30 (4):  53-58.  Abstract ( 694 )   PDF (985KB) ( 847 )   Save Physico-chemical and spectral properties of theabrownin(TB)extracted from pu-erh tea were studied using pu-erh tea as raw material. The results showed that theabrownin has a good oxidative tolerance and a poor reductive tolerance, and it is unstable under acidic, alkaline and heating conditions. UV-vis spectrum shows that theabrownin has a characteristic absorption peak at 330nm, but the 80% ethanol/water extract has characteristic absorption peaks at 330 and 380nm, respectively. IR spectrum shows that theabrownin belongs to phenolic substance and it contains hydroxyl, alkyl, carboxyl radicals and benzene ring. GPC result shows that its relative molecular weight(Mr) is large. After repeated column chromatography, the weight-average molecular weight(Mw) number-average molecular weight(Mn) and polydispersity index of the purified theabrownin are 2 878, 2 120 and 1.36, respectively. Related Articles | Metrics

Select

Effect of Bisulfite Pretreatment on Enzymatic Hydrolysis of Corn Stalk LIU Yun-yun;WANG Gao-sheng;PU Chun-gang;LIU Zhi 2010, 30 (4):  73-77.  Abstract ( 696 )   PDF (998KB) ( 948 )   Save The effects of bisulfite pretreatment conditions including pretreatment temperature, the dosage of sodium bisulfite and pH value on the efficiency of enzymatic hydrolysis of corn stalk were studied. The results showed that the dissolution quantity of enzymatic hydrolysis and glucose yield increased with sodium bisulfite increasing when temperature was 180℃ and holding time was 30min. The enzymatic hydrolysis of the substrate increased while dissolution of both lignin and hemicellulose increased, the conversion of enzymatic hydrolysis was 69.40% and the yield of glucose was 62.44% when bisulfite dosage was 7%. Efficiency of enzymatic hydrolysis increased with higher pretreatment temperature. Conversion of enzymatic hydrolysis of the substrate was achieved 81.04% and the glucose yield reached 71.91% when the temperature was 190℃, retention time was 30min, and sodium bisulfite dosage was 7%. pH Value had great effect on the efficiency of enzymatic hydrolysis. When the pH value was in the range of 4.2-4.7, the efficiency of enzymatic hydrolysis rose significantly. Related Articles | Metrics

Select

Effects of the Properties of Phosphoric Acid-activated Carbon on Adsorption Capacity of Methylene Blue ZUO Song-lin;LIU Jun-li;YANG Jian-xiao;CAI Xuan 2010, 30 (4):  1-6.  Abstract ( 722 )   PDF (973KB) ( 1493 )   Save Activated carbons of China fir(Cunninghamia lanceolata) wood origin were prepared from phosphoric acid activation in atmospheres of air, nitrogen and steam respectively, and the effects of the properties of activated carbon on the adsorption capacity of methylene blue were investigated. Porous texture of activated carbon was analyzed based on the nitrogen adsorption isotherms. Surface functional groups were determined by the Boehm method, and the adsorption isotherms of methylene blue on the activated carbons were measured. The results show that activated carbon with more developed porosity has higher adsorption capacity of methylene blue. Surface functional groups on activated carbons, especially strong acid groups, play two roles in adsorbing methylene blue. Surface functional groups facilitate the arrangement of methylene blue molecules at a more compact way on activated carbon and thus produce a positive effect on the adsorption of methylene blue. On the other hand, they can screen to some degree parts of pores that are originally accessible to methylene blue molecules and thus suppress the adsorption. Consequently, an increased concentration of surface functional groups may produce an increase in the adsorption capacity of methylene blue for the activated carbon with developed mesopores because the former role predominates, on the contrary, a decrease for the microporous activated carbon because the latter role predominates. Related Articles | Metrics

Select

Preparation of 3,4,5-Trimethoxy-benzaldehyde as Reference Substance WANG Yong-mei;CHEN Jia-hong;ZHANG Liang-liang;WU Dong-mei;XU Man;WU Zai-song 2010, 30 (4):  25-28.  Abstract ( 719 )   PDF (594KB) ( 669 )   Save Preparation, homogeneity, stability and certification of reference substance of 3,4,5-trimethoxy-benzaldehyde were studied. Highly purified reference substance of 3,4,5-trimethoxy-benzaldehyde was prepared from an industrial grade 3,4,5-trimethoxy-benzaldehyde product by NaHSO3 addition reaction followed by acidifying with H2SO4 and re-crystallization in ethanol and water. The content of 3,4,5-trimethoxy-benzaldehyde in reference substance was measured by gas chromatographic area normalization. The reference substance of 3,4,5-trimethoxy-benzaldehyde passed the test of homogeneity, and it was stable during 9 months at least. Through valuation test, 3,4,5-trimethoxy-benzaldehyde content of the reference substance was 99.42%±0.07%. Related Articles | Metrics

Select

Preparation and Characterization of Maleated Rosin/CaO Hybrid Material LI Hai-long;HUANG Qi-liang;LEI Fu-hou 2010, 30 (4):  43-47.  Abstract ( 639 )   PDF (1058KB) ( 773 )   Save The maleated rosin/CaO hybrid material was synthesized by liquid-phase method.The process conditions were investigated by orthogonal experiment. The results showed that the condition for the optimum yield was as follows: temperature 338K, reaction time 30min, Ca(NO3)2 initial concentration 0.215mol/L. Maleated rosin/CaO hybrid material was characterized by Fourier transform infrared spectrometry(FT-IR), scanning electron microscopy(SEM), energy dispersive X-ray microanalysis(EDAX) and X-ray diffraction(XRD). The results reveal that the obtained maleated rosin/CaO is crystalline compound, and chemical bonding occurs between —COO- and Ca2+. The purity of the maleated rosin/CaO hybrid material was affected by the amount ratio of sodium maleated rosinate to Ca(NO3)2, which should be controlled between 1:1.2 to 1:1.5. Related Articles | Metrics

Select

Preparation and Properties of Insoluble Dietary Fiber from Marc of Hovenia dulcis Thunb. ZHANG Cun-li;SHAO Yi-tian;ZOU Yong;CAO Ying-li 2010, 30 (4):  59-64.  Abstract ( 750 )   PDF (393KB) ( 726 )   Save Marc from Hovenia dulcis Thunb. fruit is a new cheap source of insoluble dietary fiber(IDF). To utilize this resource comprehensively, the preparation of IDF from H.dulcis marc by chemical methods and its bleaching with hydrogen peroxide(H2O2)were studied. The nutritional components and functional properties of the product were also analyzed. According to the result of single factor analysis and orthogonal test, the reasonable technology of extraction is that the marc is immersed in 1.5mol/L NaOH at 20℃ for 40min with 8:1(mL:g) ratio of liquid to material,then filtered and washed to neutrality. The residue was transferred into H2SO4 solution of pH value 2.0 with 6:1(mL:g) ratio of liquid to material and soaked at 60℃ for 60min,then washed to neutrality. The optimum processing conditions of decoloration were as follows: 5% H2O2, pH value 12, 45℃, 5h, then dried at 65℃ for 5h, milled and sieved(380μm). Under these conditions the IDF whiteness increased from 51.63% to 60.21%. The IDF yield is 74.02% and purity increased from 62.62% to 84.9 %(on dry base). Compared with the marc, water-holding capacity, oil-holding capacity, water-retention capacity and swelling capacity of IDF product increased from 2.4 to 4.3g/g, 2.3 to 3.9mL/g, 2.6 to 4.7g/g and 2.8 to 4.6mL/g, respectively. Related Articles | Metrics

Select

Determination of Content of Three Kinds of Diester-type Alkaloids in Aconitum in Jilin District by HPLC JIANG Gui-quan;GAO Wen-tao;LI Ya-rong;PANG Jiu-yin 2010, 30 (4):  85-88.  Abstract ( 703 )   PDF (607KB) ( 670 )   Save The contents of three kinds of diester-type alkaloid, namely: aconitine, mesaconitine and hypaconitine in aconitum in Jilin district were measured by high performance liquid chromatography. Results showed that content of mesaconitine has a good linear relationship(r 0.9996)in 0.0016-2.0030g/L with average recovery of 98.6%, relative standard daviation(RSD) 1.7%; The content of aconitine has a good linear relationship(r 0.9998)in 0.0016-1.0005g/L with average recovery of 97.5%, RSD 2.8%; The content of hypaconitine has a good linear relationship(r 0.999 5)in 0.0016-1.0000g/L with average recovery of 96.7%, RSD 1.9%. This method is simple, accurate and reliable, and applicable to quality control of aconitum. From comparative analysis, the contents of aconitine(0.1926%), mesaconitine(0.4658%) and hypaconitine(0.1045%) were the highest in Aconitum kusnezoffii Reichb. root. This shows that it may be adopted as the main variety of domestication and cultivation in the future. Related Articles | Metrics

Select

Optimization of Homogenated Extraction of Magnolol and Honokiol from Magnolia officinalis Using Response Surface Methodology XIE Jie;LI Hong-juan;ZHENG Xing-zong;ZHU Xing-yi;WANG Ping 2010, 30 (4):  20-24.  Abstract ( 712 )   PDF (863KB) ( 1041 )   Save Method of response surface analysis(RSA) was used for optimizing the homogenated extraction of magnolol and honokiol from Magnolia officinalis. Ethanol volume fraction, extracting time and liquid to material ratio were selected as factors based on single-factor test and a three-factor-three-level experiment design has been developed by the method of Box-Benhnken central composite design. The optimal extraction conditions were determined by the experiment of RSA. The results showed that homogenated extraction was an efficient method to extract magnolol and honokiol from M.officinalis. The optimized conditions were as follows: volume fraction of ethanol 92%, extraction time 71s, liquid/solid ratio 35:1(mL:g) and particle size 1.7mm. Under these optimum conditions, the actual extraction rate of total phenolic was 20.10mg/g(13.96mg/g of magnolol and 6.14mg/g of honokiol), matching with the predicted extraction rate of 20.32mg/g. Related Articles | Metrics