Table of Content

30 June 2009, Volume 29 Issue 3 Previous Issue    Next Issue

研究报告

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Synthesis of VC Lyral Acetal and Its Antioxidation Activity WANG Yu-min;ZHAO Zhen-dong;BI Liang-wu;LI Dong-mei;WANG Jing;GU Yan;BO Cai-ying 2009, 29 (3):  13-17.  Abstract ( 661 )   PDF (855KB) ( 636 )   Save VC lyral acetal (L-ascorbyl lyral acetal) was synthesized with VC (L-ascorbic acid) and lyral resolved in solvents N,N-dimethylformamide (DMF) andhexane as a water-carrying agent catalyzed by p-toluenesulfonic acid. VC lyral acetal, constituted of two isomers (A and B) in a ratio about 74% to 26%, was identified by its LC-MS, FT-IR and 1H NMR spectral data. The main reaction conditions are as follows:molar ratio 1 to 1.5 for lyral to VC, 5% of catalyst to mass of lyral, and stirring and refluxing for 6h. The antioxidation activity of VC lyral acetal was characterized by DPPH· (2,2-diphenyl-1-(2,4,6-tri-nitrophenyl)hydrazyl free radical) assay. The result shown that the ability of VC lyral acetal for scavenging DPPH· free radical (antioxidation activity) was similar with that of VC, but remarkably stronger than that of BHT, a common antioxidant, so the acetal would be applicable as agood oil-soluble VC-type antioxidant with latent aroma. Related Articles | Metrics

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Study on Preparation,Properties and Application of Avicel-acrylate Composite Microsphere CHEN Ri-qing;JIANG Yu;JIN Li-wei;WANG Chun-peng;CHU Fu-xiang 2009, 29 (3):  25-30.  Abstract ( 670 )   PDF (1986KB) ( 761 )   Save Avicel-acrylate composite microsphere was prepared using inverse-phase suspension polymerization under following conditions:oil phase/water phase (O/W,mass ratio)2.4:1,mass ratio of alkylphenol ethoxylate (OP) and sodium lauryl sulfate (SLS) 4:1,mass ratio of methyl methacrylate (MMA), butyl acrylate (BA) and N-hydroxymethyl acrylamide (NMA) 35:15:3, and stirring speed 200-400r/min. Results of analysis on interior structure and properties of microsphere, crystallinity of avicel before and after reaction indicated that avicel participated crosslinking reaction and was encapsulated by polyacrylate. High flexural performance of translucent yellow molding plastics with low water absorption was obtained by blending composite microspheres of 85nm diameter with polymethyl methacrylate (PMMA). Related Articles | Metrics

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Performance of Dimeric-acid-based Alcohol-soluble Polyamide Resins Used for High-gelling-resistant Printing Inks HUANG Kun;XIA Jian-ling;LI Mei;WANG Ding-xuan; 2009, 29 (3):  47-52.  Abstract ( 690 )   PDF (1007KB) ( 765 )   Save A series of polyamide resins used for printing inks were synthesized by condensation polymerization of dimeric acid with isophorone diamine (IPDA) and other monomers. Results of differential scanning calorimetry (DSC) showed that polyamides prepared from IPDAhavehigher Shorehardness and shearing strength but lower melting point (Tm) andglass transition temperature (Tg).With the increase of IPDA:ethyl diamine (EDA) ratio,gel point of the alcoholic solution of polyamide decreased,while softening point decreased firstly, then increased. The softening point of polyamide (1009I) prepared from IPDA and dimeric acid was 103℃, the viscosity of its 40% solution in 1-propanol was 208 mPa·s at 25℃, and Tg 44.2℃, Shorehardness 79, shearing strength 14.64MPa, and solubility parameter 17.87×10-3(J·m-3)1/2. Polyamide 1009I is soluble in 1-propanol, tetrahydrofuran and n-butyl acetate. Gel point of its 20% n-propyl solution is -35℃, showinggoodgelling-resistance. However,IPDA can not improve the solubility of polyamide resin in ethonal. Related Articles | Metrics

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Structural Progressing of Phenol-urea-formaldehyde Resin under Alkaline Condition LEI Hong;DU Guan-ben;PIZZI Antonio;ZHAO Wei-gang;LI Qin;FANG Qun;ZHAO Chao-chao 2009, 29 (3):  63-68.  Abstract ( 685 )   PDF (1249KB) ( 695 )   Save To find the rule of structural progressing of phenol-urea-formaldehyde (PUF) resin prepared under alkaline condition and to provide theoretical basis for PUF preparation, quantification of main functiongroups and identification of different linkages formed in PUF prepolymers were analyzed by 13CNMR spectroscopy when phenol, formaldehyde and urea were charged by several times during the preparation of PUF. Results showed that PUF prepolymers obtained by different charging methods showed similar chemical structure with almost same type of linkage but showed difference in structural constitution. Under definite materials' ratio in this experiment, the final reaction progresses of synthesizing PUF resins were almost similar. The charging of formaldehyde by several times could decrease the formation of ether and was in favor of simplifying the reaction. Results of thermo-mechanical analysis (TMA) exhibited that curing performance of PUF prepolymers were not much affected by different charging methods. Related Articles | Metrics

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Separation of Alkali-lignin Grafted Sodium Acrylate Copolymers WANG Bai-liang;ZHANG Wei-ying;LI Xiao;CHENG Xian-su 2009, 29 (3):  107-110.  Abstract ( 584 )   PDF (949KB) ( 702 )   Save Grafted copolymerization of alkali-lignin with sodium acrylate was carried out using K2S2O8 and Na2S2O3 as initiator, and method of separating alkali-ligningrafted copolymers was investigated. The results showed that acidification followed by centrifugation could well separate out thegrafted copolymers from the crude products. At neutralization degree of 105%-115% and centrifugation rate of 6000r/min, the separation could be done in 9min. Using the FT-IR spectra, the separated product was verified to begrafted copolymers. Related Articles | Metrics

综述评论

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Structure-function Relationships of Cellulase and its Encoding Gene YU Han-ying;LIU Xing-zhong 2009, 29 (3):  120-126.  Abstract ( 646 )   PDF (1098KB) ( 1096 )   Save Cellulose is an important potential bio-energy resource. One of the bases of utilizing the resource is to study the structure and function of cellulase and the encoding DNA sequence to elucidate the regulation of thegene expression and thehydrolysis mechanism of the enzyme. Recent advances in the structure-function relationship of cellulase and its encodinggene were reviewed and future research was prospected. Related Articles | Metrics

研究报告

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Study on Regulation of Iridoid Glycosides Synthesis in Gardenia jasminoides Ellis Callus YANG Kui;CHEN Fang 2009, 29 (3):  73-77.  Abstract ( 721 )   PDF (928KB) ( 560 )   Save To promote the development of normalized cultivation of Gardenia jasminoides Ellis, the rule of iridoidglycosides synthesis in G. jasminoides callus was studied by plant cell culture technology,HPLC and UV. Thegrowth of G.jasminoides callus and the accumulation of iridoidglycosides are not proceeded synchronically, callusgrows first and then its secondary metabolic products are synthesized. Sucrosehas obvious effect to regulate the synthesis of iridoidglycosides.When concentration of sucrose is between 3% and 8%, thegrowth of callus and synthesis of iridoidglycosides aregreatly increased. The addition of organics such as beef cream and peptone in culture medium could promote callusgrowth, but inhibited the accumulation of iridoidglycosides. Callusgrowth could be induced when concentrations of Fe2+ and Mn2+ were properly increased in Murashige & Skoog(MS) culture medium. Mn2+ could increase accumulation of iridoidglycosides, but Fe2+had no obvious effect on accumulation of iridoidglycosides.Thegrowth of G. jasminoides callus and accumulation of iridoidglycosides were suppressed when ammonium salt or nitrate was used alone in culture medium as nitrogen source. Under the condition of c(NH4+):c(NO3-) 1:1 and total nitrogen source 60 mmol/L, both total biomass and amount of iridoidglycosides arrived to the maximum. Increase of KH2PO4 in culture medium between 0 and 0.5 mmol/L couldgreatly increase callusgrowth which arrived to the maximum at 1.5 mmol/L. Related Articles | Metrics

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Analysis of Artemisinin from Artemisia annua L. by HPLC-UV Using Pre-column Derivatization ZHANG Rong-shu;WANG Yan;ZHAO Min 2009, 29 (3):  115-119.  Abstract ( 660 )   PDF (886KB) ( 770 )   Save Effect of pre-column derivatization on determination of artemisinin from Artemisia annua by HPLC-UV was studied. The modified determinatin method of HPLC-UV with pre-column derivatization was established as follows:the standard solution of artemisinin (or its extract) was supplementally added to 15.0mL with 95% ethanol, then added with 0.2% NaOH 8.0mL, andheated in a water bath at 45℃ for 30min. After it was cooled to room temperature, acidified with 0.08mol/L HAc 10mL and shaked. Then it was filled to marked volume of 50 mL with 95% ethanol, separated by HPLC, and detected at 260nm. The methodgave a wide range of linearity in the concentration range from 0 to 200mg/L. The relative standard deviation(RSD) was 1.40% and average recovery(n=5) was 100.1%. The method was used to determine artemisinin extracted from introduced sample. The result demonstrated that the RSD of stabilization and reproducibility were 4.35% and 4.96%, respectively. It fully proved that the method is simple, stable and reproducible. Related Articles | Metrics

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Study on Optimum Process for Extracting Coumarin from Cortex fraxini by Orthogonal Test ZHANG Qi;ZHANG Xin-shen;DAI Yong 2009, 29 (3):  89-92.  Abstract ( 633 )   PDF (798KB) ( 796 )   Save The optimum process of extracting fraxin, esculin and esculetin as active coumarin from Cortex fraxini(Qinpi) bark was studied. A rapid and simple method used to determine fraxin, esculin and esculetin in C. fraxini by capillary zone electrophoresis(CZE) was described. The effects of EtOH volume fraction, extracting times,extracting time,and ratio of material to liquor on extraction yield of 3 kinds of coumarin were investigated by orthogonal test. The optimum extracting conditions confirmed by the index of extraction yield of 3 kinds of coumarin were as follows:ratio of material to liquor 1:9(g:mL),three times of reflux extraction with 65% EtOH, each extraction 1h. The yield of total coumarin was 3.61% Related Articles | Metrics

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Ultrasonic-assisted Solvent Extraction of Ursolic Acid and Oleanolic Acid from Micromeria biflora Benth. LI Kun-ping;PAN Tian-ling;BI Yong-guang;PAN Tian-ming 2009, 29 (3):  111-114.  Abstract ( 719 )   PDF (671KB) ( 771 )   Save Ultrasonic-assisted solvent extraction of oleanolic acid and ursolic acid from Micromeria biflora Benth., was studied and compared with solvent reflux extraction. The solvent was selected based on single factor experiments and the extracting process was optimized by orthogonal test. Using ethanol as solvent, the best results can be achieved under the following conditions:90% (volume fraction)ethanol, solid-liquid ratio 1:15, ultrasonic power 360W at 40kHz, extracting for 2 times, each time 20min, total yield of oleanolic acid and ursolic acid was 0.732%; for solvent reflux extraction the optimal parameters were:solid-liquid ratio 1:15, extracting 2 times at 90℃, each time 30min, total yiled of oleanolic acid and ursolic acid was 0.706%. Ultrasonic-assisted solvent extraction can effectively extract oleanolic acid and ursolic acid from M. biflora with advantages of simple operation andhigh efficency. Related Articles | Metrics

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Study on Anthraquinone Constituents in the Seed of Cassia tora L. JIA Zhen-bao;CHEN Wen-wei;JIANG Jia-xin;DING Xiao-lin 2009, 29 (3):  100-102.  Abstract ( 641 )   PDF (455KB) ( 795 )   Save Anthraquinone constituents in the seed of Cassia tora L. were investigated. Seven anthraquinone compounds were isolated from chloroform extract of the seed of C. tora with silicagel column chromatography. Their structures were elucidated through physiochemical and spectral analyses. They were identified to be chrysophanol (Ⅰ), physcion (Ⅱ), emodin (Ⅲ), chryso-obtusin (Ⅳ), 8-O-methylchrysophanol (Ⅴ), 1-O-methylemodin (Ⅵ) and 1,2-dimethoxy-8-hydroxy-3-methyl-9,10-anthraquinone (Ⅶ). Compounds Ⅴ-Ⅶ were obtained from thisgenus of plant for the first time. Related Articles | Metrics

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Extraction and Separation of Huperzine A from Huperzia serrata(Thunb.)Trev. LI Yu-shan 2009, 29 (3):  103-106.  Abstract ( 613 )   PDF (791KB) ( 678 )   Save The optimum technological conditions of extractinghuperzin A from Huperzia serrata(Thunb)Trev. by single factor test and orthogonal design method were studied. The optimum extraction conditions were as follows:ratio of ethanol to sample 25:1(mL:g), extraction time 3h, extraction temperature 60℃. The optimum purification conditions were as follows:ethanol volume fraction 80%, D101 macroporous adsorption resin eluted under room temperature, to obtain an overall yield of 0.3% and a purity of 10%. Related Articles | Metrics

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Study on Synthesis of 2-Hydroxy-3-Pinanone from α-Pinene ZANG Yun-xiao;HA Cheng-yong;SHEN Dan-dan;SHEN Min-min 2009, 29 (3):  69-72.  Abstract ( 845 )   PDF (708KB) ( 899 )   Save The synthetic technology of 2-hydroxy-3-pinanone from α-pinene was studied. Affecting factors such as cosolvent were investigated. The optimum technology was obtained as follows:using tert-butyl alcohol as cosolvent, taking no measure to change pH value, reaction temperature 0-5℃, ratio of n(KMnO4):n(α-pinene) 1.7:1-1.8:1, addition of potassium permanganate after α-pinene. The structure of the product was characterized by IR, MS, 1HNMR and 13CNMR. The yield of 2-hydroxy-3-pinanone was up to 60%, ashigh as the literatures reported before. The recovery of tert-butyl alcohol washigher than 90%, which washigher than that of acetone (70%), the cosolvent used ever before. Related Articles | Metrics

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Effect of Transesterification Conditions on Unsaturation Degree of Micro-biodiesel DAI Chuan-chao;ZHANG Bo;YANG Qi-yin;LI Pei-dong 2009, 29 (3):  43-46.  Abstract ( 678 )   PDF (659KB) ( 670 )   Save To produce micro-biodiesel from microbial oil, transesterification conditions were studied using oil from Rhodotorulaglutinis as raw material using unsaturation index of fatty acid (UIFA)as index. The result showed that the conditions of:reaction temperature 55℃, molar ratio of methanol to oil 40:1, H2SO4 75% based on oil weight, and time more than 6h. These conditions are favourable to accomplish the process of tansesterification sufficiently. Related Articles | Metrics

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Homogenated Extraction of Oligomeric Proanthocyanidins from Larch Bark and Its Optimization by Response Surface Methodology JIA Jia;YANG Lei;ZU Yuan-gang 2009, 29 (3):  78-84.  Abstract ( 583 )   PDF (1371KB) ( 812 )   Save The method ofhomogenated extraction was used to extract oligomeric proanthocyanidins from larch bark, using yield and purity of oligomeric proanthocyanidins as index. Based on single-factor experiment, Box-Behnken experiment design was applied to optimize the extraction process. The optimized conditions were obtained as follows:70% ethyl alcohol as solvent, ratio of solid to liquid 1:15,homogenation time 15min and extraction 4 times. Under these conditions, the yield and purity of oligomeric proanthocyanidins from larch bark were 17.33% and 75.46%, respectively. Related Articles | Metrics

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Determination of Rutin Content in Syzygium jambos Leaves by Differential Pulse Voltammetry LUO Ji-wen;CHEN Yuan;LI Zhong-liang;WEI Qing-min 2009, 29 (3):  85-88.  Abstract ( 652 )   PDF (794KB) ( 618 )   Save Voltammetric behavior of rutin at activatedglassy carbon electrode(GCE) was studied .The voltammegram shows a pair of redox peaks when potential scans in a phosphate buffer(PBS) containing rutin. In the phosphate solution of pH value 4.0, the peak current of differential pulse voltammetry (DPV) for rutin rises as its concentration increases, andgood linearity was observed over a range of 0.8-50μmol/L with a detection limit of 0.3μmol/L. This method was developed for the determination of rutin content in plant extracts which is simple and sensitive withgood repeatability. A sample of S.jambos leave was determined with this method in standard-addition way to obtain the rutin content of 15.7mg/g with RSD of 3.3%. Related Articles | Metrics

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Preparation and Properties of Waterborne Polyurethane- acrylate-epoxidized Soybean Oil Hybrid Emulsion GUO Wen-jie;FU He-qing;SITU Yue;XU Li;LAN Ren-hua;CHEN Huan-qin 2009, 29 (3):  31-37.  Abstract ( 708 )   PDF (1237KB) ( 693 )   Save Waterborne polyurethane(WPU) was modified by epoxidized soybean oil(ESO), methyl methacrylate (MMA), butyl acrylate (BA) and 2-hydroxypropyl acrylate (HPA), to obtain polyurethane-acrylate-ESOhybrid emulsion whichhas a core-shell structure with acrylate as core and polyurethane as shell. The structure and properties of thehybrid emulsion were characterized by Fourier transform infrared (FT-IR) spectra and particle size analyzer. Effects of process parameters on polymerization and dosages of ESO and MMA on properties of thehybrid emulsion and its films were investigated. Results showed that the optimal process was as follows:temperatures of prepolymerization, chain-extending, neutralization and emulsion polymerization were 80,70,40 and 70℃, respectively, times of prepolymerization, chain-extending, emulsion polymerization were 1.5,4-5 and 4-5h,respectively, and rotation rate was 6000r/min.When contents of ESO and MMA were 4%-6% and 30%,respectively,hybrid emulsion with small particle size andgood store stability was prepared. Its filmhadgood media resistance and low water absorption. Related Articles | Metrics

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Optimized Ultrasonic Extraction of Main Phenolic Glycosides and Aglycones from Acanthopanax senticosus(Rupr.et Maxim.)Harms YANG Lei;GAO Yan-feng;LIU Yang;HAO Jing-wei;ZU Yuan-gang 2009, 29 (3):  93-99.  Abstract ( 695 )   PDF (1662KB) ( 721 )   Save Ultrasonic-assisted extraction was adopted to extract main phenolicglycosides and aglycones from Acanthopanax senti-cosus(Rupr.et Maxim.)Harms, including the active ingredients such as syringin, eleutheroside E and isofraxidin. According to Box-Behnken central composite experiment design, the extracting process was designed with three factors at three levels. The three factors are volume fraction of ethanol, extraction time and ultrasonic power. The yield and purity of three target products were selected as responses respectively to optimize the extracting process. Response surface analysis (RSA) was applied to determine the effect of central composite experiment design. The optimum conditions of RSA were as follows:volume fraction of ethanol 59.66%, ratio of solid to liquid 1:6(g:mL), ultrasonic time 44.9min, ultrasonic power 150W. Under the optimum conditions the content of main phenolicglycoside and aglycone was 2.923%, of which the purity of syringin was 1.3%, isofraxidin was 0.093%, eleutheroside E was 1.53%, extract yield was 6.77%. Related Articles | Metrics

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Isolation and Structrual Identification of Oleuropein in Leaves of Ascolano Olive Introduced from Greece WANG Cheng-zhang;GAO Cai-xia;YE Jian-zhong;CHEN Xi-juan;JIANG Cheng-ying 2009, 29 (3):  53-57.  Abstract ( 645 )   PDF (933KB) ( 815 )   Save There are many antioxidants in olive leaves such as oleuropein, flavonoids and polyphenols. Leaves of Ascolano olive introduced from Greece were successively extracted with EtOH-H2O(60:40), petroleum ether and ethyl acetate. The ethyl acetate fraction was subjected to silicagel column chromatography, then treated with preparative HPLC to obtain a compound with content of 95%, which was identificated to be oleuropein by UV,IR,MS and NMR. Oleuropein could be acidicallyhydrolyzed togivehydroxytyrosol and elenolic acid as main products. Related Articles | Metrics

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Preparation of Water-emulsified Terpinene-maleic Ester Type Epoxy Resin by Phase-inversion Emulsification LIU Yang;HUANG Huan;KONG Zhen-wu;WU Guo-min;CHEN Jian 2009, 29 (3):  18-24.  Abstract ( 725 )   PDF (1334KB) ( 785 )   Save A water-emulsified terpinene-maleic ester type epoxy resin (TME) was prepared by phase-inversion emulsification, and a self-designed emulsifier prepared by TME modified with PEG was used as reactive emulsifier in this emulsion. The relationship between conductivity and micro-morphology in the emulsifying process was analyzed. The influences of the emulsifier mass fraction, emulsifying temperature, stirring speed on the volume average particle diameter (D), consistency of particle size distribution, centrifugal stability and viscosity of the emulsion were also discussed. The results show that the optimum conditions for preparing this type of water-emulsified epoxy resin were as follows:emulsifier mass fraction 16.7%, at 60℃ and 500-900r/min, and solid content about 45%. Under these conditions, particle diameter of the emulsion is among 100-400nm, average particle size is about 200 nm, and viscosity is about 850mPa·s, epoxy value is 0.11mol/100g. Related Articles | Metrics

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Study on Synthesis of Rhodiola crenulata Polysaccharides-iron Complex and Its Inhibition Effect on Iron-deficiency Anemia HUANG Jia-kun;JIANG Kai-nian;WANG Feng-juan;CUI Zhi-bin;HAN Yong-ping 2009, 29 (3):  58-62.  Abstract ( 588 )   PDF (1036KB) ( 727 )   Save Rhodiola crenulata polysaccharides-iron complex was synthesized by trisodium citrate-FeCl3 method. Orthogonal design was used to optimize the synthesis conditions of polysaccharide-iron.The effect of R.crenulata polysaccharide-iron complex on iron-dfficiency anemia was investigated by animal experiment. Results showed:A R.crenulata polysaccharide-iron complex with β-FeOOH iron nuclide was successfully synthesized. The optimized conditions were confirmed as follows:raw material 2g, m(polysaccharides):m(trisodium citrate)4:3,pH value 8.5, temperature70℃.The yield 0.55g and the mass fraction of iron 23.31% were obtained. The solution of polysaccharide-iron complex was kept clear, without deposits at pH value 3-11.Fe(Ⅲ) in polysaccharide-iron complex could be basically reduced into Fe(Ⅱ) within 7h by ascorbic acid. The polysaccharide-iron complex can increase red cell(Hb),hemoglobin(RBC),serum iron(SI),splenic index, splenic mass and mass of mice with iron-deficiency anemia (IDA). Compared with modelsgroup, there were significant differences. Related Articles | Metrics

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Study on Composite Adhesive of Hydroxymethylated Lignosulfonate/Phenol-formaldehyde Resin with Low Free Formaldehyde MU You-bing;WANG Chun-peng;ZHAO Lin-wu;CHU Fu-xiang 2009, 29 (3):  38-42.  Abstract ( 809 )   PDF (1185KB) ( 963 )   Save The lignin-phenol-formaldehyde resin adhesive was prepared by blending modified technical lignin with phenol-formaldehyde resin. Lignin was modified byhydroxymethylation to increase reactivity and the resultedhydroxymethylated lignin was analyzed by FT-IR and 13CNMR. The optimal conditions ofhydroxymethylation are as follows:lignin-formaldehyde mass ratio 3:1, catalyst dosage 0.25%.When 40% of phenol-formaldehyde resin(PF)was replaced byhydroxymethylated lignin, the adhesive thus formulated showed low free formaldehyde, and the bonding strength could meet the standard of type I plywood. Related Articles | Metrics

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Preparation and Properties of Modified Rosin-condensed Tannins Esters DUAN Wen-gui;GENG Zhe;ZHANG Jia-yan;SHEN Chang-mao 2009, 29 (3):  6-12.  Abstract ( 721 )   PDF (681KB) ( 570 )   Save Under microwave irradiation and in the presence of pyridine as catalyst, a series of modified rosin-condensed tannins esters were synthesized by O-acylation reaction between condensed tannins and modified rosin chloride. The target products were analyzed and characterized by means of UV, IR, TG-DTA and elemental analysis. In addition, antioxidant activity and surface activity of the esters were determined. The test results showed that modified rosin-condensed tannins estershave better oil-solubility in peanut oil and better antioxidant activity than those of condensed tannins, and that of dehydroabietic acid-condensed tannins ester (DHA-MET) was the best of all. The surface activity was evaluated by surface tension, emulsifying power and foaming ability. The results indicated that sodium salts of the modified rosin-condensed tannins esters, especially sodium salts of thehydrogenated rosin-condensed tannins esters,had the superior surface activity. Related Articles | Metrics

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Synthesis of Alky Gallate by Tannase Immobilized in Microemulsion-based-gels WANG Zheng 2009, 29 (3):  1-5.  Abstract ( 657 )   PDF (970KB) ( 688 )   Save Alkygallate is a kind of antioxidant and possesses patient therapy value. However, it was synthesized by chemical method, whichhas defects of serious circumstance containment and low productivity. To avoid these defects, the method of biosynthesis for alkygallates was established using microemulsion-based-gels(MBGs) andgallic acid was esterificated catalytically with different alcohols by tannase immobilized in microemulsion-basedgels,from which propylgallate (PG), butylgallate (BG) and amylgallate (AG) were successfully synthesized. Salt-salthydrate (Na2SO4-Na2SO4·10H2O) could control and regulate water activity effectively, the ability was related to amount of salt-salthydrate. The conversion ofgallic acid was improved significantly when salt-salthydrate was added into reaction system. The conversion ofgallic acid reached 78.2% and 85% respectively when pH value was 6 and amount of Na2SO4-Na2SO4·10H2O(ratio 1:1) was 1.8 g under the temperature of 28℃ in MBGs reaction systems for PG synthesis and BG synthesis, compared to the control, increasing by 93.0% and 129.7%,respectively. The conversion ofgallic acid reached 72.4% when pH value was 6 and amount of Na2SO4-Na2SO4·10H2O(ratio 1:1)was 2.4g under the temperature of 28℃ in MBGs reaction system for AG synthesis, compared to the control, increasing by 103.7%. Related Articles | Metrics