Abstract: A series complexes of polymerized rosin (PR) or grafted copolymers of polyvinyl alcohol with PR (PVA-g-PR) were synthesized. These complexed polymerized rosins were used as carriers for immobilizing amylase and their properties were investigated. The experimental results showed that amylase immobilized on calcium-complexed PR (PRCa²⁺) had good reusability, the activity was retained 30.8% after being used 5 times. Optimum operating temperature was 70℃ for immobilized amylase which was 30℃ higher than that of free amylase. Optimum pH value was 5.0 for immobilized amylase and 6.0 for free amylase. K_m (Michaelies-Menten constant) of immobilization amylase was 1.69×10^-2 kg/L, which is lower than 2.90×10^-2 kg/L of free amylase.

Key words: calcium-complexed polymerized rosin, amylase