Abstract: The new method of isolation and purification of (+)-pinoresinol-di-β-D-glucopyranoside(PG)and (+)-syringaresinol di-β-D-glucopyranoside (SG) from Eucommia ulmoides Oliv.was studied. PG and SG were isolated through column chromatography packed with different resins, and the best isolation condition was established according to the results of HPLC analysis. Ethanol extracts was concentrated, centrifugated and then separated by column packed with D101 resin under alkaline condition.The isolated yield was 1.74%, the contents of PG and SG were 8.139 7% and 2.943 1%, respectively. The extract was further purified by means of Sephadex LH-20 column chromatography, followed by preparative HPLC and recrystallization, to obtain two crystals which were characterized as PG and SG by melting point determination, HPLC, TLC, IR, UV and ～1H NMR analysis. Compared with tradition method, the new isolation technology was simple and safe with high yield of product.

Key words: Eucommia ulmoides Oliv., (+)-pinoresinol-di-β-D-glucopyranos, (+)-syringaresinol-di-β-D-glucopyranoside