Abstract: Cellulase system contains a series of complex components,in which C_x-enzyme is a main member. Traditional assaying method of enzyme activity leads to lots of system mistakes attributable to low and alternative substrate concentration. On account of that, a reasonable new way to show enzyme activity by determining ν_max is brought out. The research of this method focuses on the analysis of enzyme activity of C_x-enzyme expressed by Trichoderma koningii AS3.4262 in different concentrations of substrate. The data have been calculated under multiple regression to get initial reaction rates of different substrate concentrations. The values of constant (K_m) and maximum reaction rate (ν_max) are obtained by using Lineweaver-Burk chart. The benefit of this improved method is to express enzyme activity regardless of varied substrate concentrations.

Key words: C_x-enzyme, enzyme activity, CMC-Na, Trichoderma koningii