木瓜籽肽-锌螯合物的制备、结构表征及稳定性研究

doi:10.3969/j.issn.0253-2417.2023.05.010

Abstract

Abstract:

In order to improve the stability and bioavailability of Zn²⁺, the Chinese quince seed peptide(CQSP) was prepared by enzymatic hydrolysis using Chinese quince seed as raw material. Five peptide fractions with different molecular weight ranges were obtained by ultrafiltration, and they were applied for preparation of CQSP-Zn chelate. The effects of protease types and peptide molecular weight on the chelation rate of Zn²⁺ were investigated. The amino acids composition analysis, Fourier transform infrared(FT-IR) spectroscopy, ultraviolet-visible(UV-Vis) spectroscopy and fluorescent spectroscopy were used to identify the structural characteristics of the chelate, and the solubility of CQSP-Zn at different pH values as well as digestion stages were also studied. The results indicated that the CQSP with the molecular weight less than 1 000 u hydrolyzed by alkaline protease presented the highest Zn²⁺ chelating rate(83.26%). The analysis of FT-IR spectroscopy, UV-Vis spectroscopy and fluorescent spectroscopy revealed the interaction between CQSP and Zn²⁺, and the -NH, CH₂, C=O, C-N, -COOH in CQSP might participate in the chelating of Zn²⁺. Compared with the inorganic ZnSO₄, CQSP-Zn showed higher solubility at pH 2.0-8.0 and simulated gastrointestinal digestion procedure. When the pH value was 8.0, the Zn²⁺ solubility of CQSP-Zn was 73.85%, which was 11.19 times higher than that of ZnSO₄. In the simulated intestinal fluid with a pH value of 7.5, the Zn²⁺ solubility of CQSP-Zn was 65.86% at 0 minute of digestion, significantly higher than ZnSO₄(16.24%), demonstrating potential application of CQSP-Zn as Zn²⁺ supplement.

Key words: peptide, chelate, simulate digestion, bioavailability

CLC Number:

TQ35
TS202.1

Yejun DENG, Lixin HUANG, Caihong ZHANG, Pujun XIE. Preparation, Structural Characterization and Stability Study of Chinese Quince Seed Peptide-Zinc Chelate[J]. Chemistry and Industry of Forest Products, 2023, 43(5): 73-80.

Figures/Tables 7

Table 1

Table 2

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References 31

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	条件 conditions	Zn²⁺螯合率¹⁾/% Zn²⁺ chelating rate
蛋白酶种类 protease species	碱性蛋白酶alkaline protease	70.84±1.747^a
	木瓜蛋白酶papain	38.98±0.671^b
	胰蛋白酶trypsin	37.37±1.528^b
	菠萝蛋白酶bromelain	36.99±1.026^b
	风味蛋白酶flavourzyme	24.01±1.275^c
肽分子质量 molecular weight of peptides	≤1 000 u	83.26±1.384^a
	>1 000-3 000 u	75.45±1.482^b
	>3 000-5 000 u	72.56±1.110^b
	>5 000-10 000 u	64.35±0.825^c
	＞10 000 u	49.67±0.909^d

氨基酸种类 type of amino acids	CQSP/%	CQSP-Zn/%	氨基酸种类 type of amino acids	CQSP/%	CQSP-Zn/%
天冬氨酸Asp	10.46	12.37	异亮氨酸Ile	6.56	4.27
苏氨酸Thr	2.35	2.03	亮氨酸Leu	7.20	4.06
丝氨酸Ser	3.49	3.74	酪氨酸Tyr	2.55	1.06
谷氨酸Glu	21.88	23.45	苯丙氨酸Phe	4.40	2.82
甘氨酸Gly	5.25	5.71	组氨酸His	4.80	6.74
丙氨酸Ala	3.13	2.13	赖氨酸Lys	1.90	3.41
胱氨酸Cys	0.86	0.69	精氨酸Arg	9.65	14.63
缬氨酸Val	4.35	3.53	脯氨酸Pro	6.61	5.58
蛋氨酸Met	4.56	3.83

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Preparation, Structural Characterization and Stability Study of Chinese Quince Seed Peptide-Zinc Chelate

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