姜黄素磁性印迹固相萃取材料的制备及其应用研究

doi:10.3969/j.issn.0253-2417.2014.06.006

林产化学与工业 ›› 2014, Vol. 34 ›› Issue (6): 37-43.doi: 10.3969/j.issn.0253-2417.2014.06.006

姜黄素磁性印迹固相萃取材料的制备及其应用研究

刘玉楠¹, 张朝晖^1,2, 黄小花¹, 饶维¹

1. 吉首大学化学化工学院, 湖南吉首 416000;
2. 湖南大学化学生物传感与计量国家重点实验室, 湖南长沙 410082

收稿日期:2013-01-08 修回日期:2014-09-04 出版日期:2014-12-25 发布日期:2015-08-18
通讯作者: 张朝晖,教授,硕士生导师,主要从事印迹色谱分析和电化学分析研究;E-mail:zhaohuizhang77@163.com. E-mail:zhaohuizhang77@163.com
作者简介:刘玉楠(1989-),女,贵州湄潭人,硕士生,主要从事印迹色谱分析研究工作
基金资助:
国家自然科学基金资助项目(21005030,21267010)

Preparation and Application of Curcumin Magnetic Molecularly Imprinted Solid-phase Extraction Material

LIU Yu-nan¹, ZHANG Zhao-hui^1,2, HUANG Xiao-hua¹, RAO Wei¹

1. College of Chemistry and Chemical Engineering, Jishou University, Jishou 416000, China;
2. State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, China

Received:2013-01-08 Revised:2014-09-04 Online:2014-12-25 Published:2015-08-18

摘要/Abstract

摘要： 以二氧化硅包覆的四氧化三铁为基材,姜黄素为模板分子,甲基丙烯酸为功能单体,乙二醇二甲基丙烯酸酯为交联剂,采用热聚合技术制备出姜黄素磁性印迹聚合物.采用扫描电镜、差热分析、样品振动磁强计和红外光谱等多种技术对该磁性印迹聚合物的形态、性能和化学结构进行分析和表征,结果表明,在磁性四氧化三铁表面成功制备出姜黄素磁性印迹聚合物.结合高效液相色谱分析技术,对该磁性印迹聚合物的吸附性能进行探讨,该磁性印迹聚合物对姜黄素表现出特异性吸附性能,最大吸附容量计算值为36.2 mg/g;相对于大黄酸和大黄素,姜黄素的选择因子(β)分别是2.0和2.2.结合磁固相萃取技术与液相色谱检测技术,实现了姜粉样品溶液中姜黄素的分离、富集和检测,回收率为99.2%~107%.

关键词: 磁性印迹聚合物, 姜黄素, 磁固相萃取技术, 高效液相色谱技术

Abstract: Magnetic molecularly imprinted polymer(MIP)was prepared by using magnetic iron oxide as the carrier, curcumin as the template molecule, methacrylic acid as the functional monomer and ethylene glycol dimethacrylate as the cross-linker. The Fe₃O₄@SiO₂@MIP was characterized by scanning electron microscope, thermogravimetric analysis, vibrating sample magnetometer and infrared spectroscopy. The results showed that the Fe₃O₄@SiO₂@MIP was prepared on magnetic iron oxide surface successfully. The adsorption properties were investigated by high performance liquid chromatography. The results demonstrated that the Fe₃O₄@SiO₂@MIP exhibited specific selectivity toward curcumin. The maximum adsorption capacity on Fe₃O₄@SiO₂@MIP was 36.2 mg/g, and the selectivity factors of curcumin toward rhein and emodin were 2.0 and 2.2, respectively. Combined with magnetic solid phase extraction and high-performance liquid chromatography technology, the Fe₃O₄@SiO₂@MIP was used to separate, enrich and detect curcumin in ginger powder, sample solution successfully with the recoveries of 99.2%-107%.

Key words: magnetic imprinted polymer, curcumin, magnetic solid phase extraction, high-performance liquid chromatography

中图分类号:

TQ35

刘玉楠, 张朝晖, 黄小花, 饶维. 姜黄素磁性印迹固相萃取材料的制备及其应用研究[J]. 林产化学与工业, 2014, 34(6): 37-43.

LIU Yu-nan, ZHANG Zhao-hui, HUANG Xiao-hua, RAO Wei. Preparation and Application of Curcumin Magnetic Molecularly Imprinted Solid-phase Extraction Material[J]. Chemistry and Industry of Forest Products, 2014, 34(6): 37-43.

参考文献

[1] 余美荣,蒋福升,丁志山,等.姜黄素的研究进展[J].中草药,2009,40(5):828-831.
[2] 袁鹏,陈莹,肖发,等.姜黄素的生理活性及在食品中的应用[J].食品工业科技,2012,33(14):371-375.
[3] 许实波,唐孝礼.姜黄素的药理作用研究概况[J].中草药,1991,22(3):140-141,139.
[4] 段玉清,张海晖,徐菲菲,等.分子印迹-固相萃取法选择性分离大黄酸的研究[J].林产化学与工业,2009,29(5):35-40.
[5] ZHANG Sheng-xiao,NIU Hong-yun,HU Zheng-jun,et al.Preparation of carbon coated Fe₃O₄ nanoparticles and their application for solid-phase extraction of polycyclic aromatic hydrocarbons from environmental water samples[J].Journal of Chromatography A,2010,1217(29):4757-4764.
[6] 聂小安,常侠,蒋剑春,等.磁性固体酸催化剂的合成及其酯化催化活性的研究[J].林产化学与工业,2011,31(2):1-6.
[7] 刘玉楠,张朝晖,张明磊,等.大黄酸磁性印迹聚合物的制备及其应用研究[J].化学通报,2012,75(9):842-847.
[8] 刘玉楠,张朝晖,陈星,等.三聚氰胺磁性印迹固相萃取材料的制备及其应用[J].应用化学,2013,30(10):1222-1230.
[9] ZHANG Zhao-hui,ZHANG Ming-lei,LIU Yu-nan,et al.Preparation of L-phenylalanine imprinted polymer based on monodisperse hybrid silica microsphere and its application on chiral separation of phenylalanine racemates as HPLC stationary phase[J].Separation and Purification Technology,2012,87:142-148.
[10] QIN Lei,HE Xi-wen,LI Wen-you,et al.Molecularly imprinted polymer prepared with bonded β-yclodextrin and acrylamide on functionalized silicagel for selective recognition of tryptophan in aqueous media[J].Journal of Chromatography A,2008,1187(1/2):94-102.
[11] 陈令允,李凤生,姜炜,等.强磁性纳米Fe₃O₄/SiO₂复合粒子的制备及其性能研究[J].材料科学与工程学报,2005,23(5):556-559.
[12] LI Zhu,DI Pan,LI Ding,et al.Mixed hemimicelles SPE based on CTAB-coated Fe₃O₄/SiO₂ NPs for the determination of herbal bioactive constituents from biological samples[J].Talanta,2010,80(5):1873-1880.

姜黄素磁性印迹固相萃取材料的制备及其应用研究

Preparation and Application of Curcumin Magnetic Molecularly Imprinted Solid-phase Extraction Material

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