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林产化学与工业

林产化学与工业 ›› 2015, Vol. 35 ›› Issue (3): 114-120.doi: 10.3969/j.issn.0253-2417.2015.03.020

• 研究报告 • 上一篇    下一篇

南方红豆杉菌根菌液体纯发酵培养产紫杉醇及10-去乙酰巴卡亭Ⅲ

龚雪元1, 杜亚填1, 刘姣1, 张翔宇2,3   

  1. 1. 吉首大学 林产化工工程湖南省重点实验室, 湖南 张家界 427000;
    2. 毕节市科技局 中药 产业发展办公室, 贵州 毕节 551700;
    3. 毕节市科技局 中药研究所, 贵州 毕节 551700
  • 收稿日期:2014-04-22 出版日期:2015-06-25 发布日期:2015-07-03
  • 通讯作者: 杜亚填,副教授,硕士生导师,研究方向为林产资源工程;E-mail:duyatian6688@163.com。 E-mail:duyatian6688@163.com
  • 作者简介:龚雪元(1987—),男,湖北襄阳人,硕士生,主要从事药用植物组织培养与新型生物反应器开发的研究
  • 基金资助:
    湖南省高校产业化项目(2011CY014);吉首大学校级课题(12Jdy12042)

Paclitaxel and 10-DAB Produced by Liquid Fermentation with Taxus chinensis var.mairei Mycorrhizal Fungus

GONG Xue-yuan1, DU Ya-tian1, LIU Jiao1, ZHANG Xiang-yu2,3   

  1. 1. Key Laboratory of Hunan Forest Products and Chemical Industry Engineering, Jishou University, Zhangjiajie 427000, China;
    2. Bijie Office of Traditional Chinese Medicine Industry Development, Science and Technology Bureau, Bijie 551700, China;
    3. Bijie Institute of Traditional Chinese Medicine, Bijie 551700, China
  • Received:2014-04-22 Online:2015-06-25 Published:2015-07-03

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摘要： 从南方红豆杉菌根菌中筛选出产紫杉醇和紫杉烷类化合物10-去乙酰巴卡亭Ⅲ(10-DAB)的高产菌株,对分离获得的21株菌株进行了液体发酵培养实验,通过TLC、HPLC及LC-MS检测发现,有10株菌株具有产紫杉醇及紫杉烷类化合物的能力,其中4株菌株中产10-DAB分别为260.05、92.88、393.27、70.52 μg/L,但未检出产紫杉醇;6株中产紫杉醇分别为434.94、1 242.19、200.65、360.77、317.90、29.82 μg/L,但未检出产10-DAB,说明南方红豆杉的各种菌根真菌虽具有合成紫杉醇或紫杉烷类化合物的能力,但各自所合成化合物有差异,不同种类的菌根菌可能分别参与了紫杉醇生物合成的不同环节,即南方红豆杉菌根高含量紫杉醇的生物合成可能是多种菌根菌协同参与作用的结果,且其中的高产菌株具有发酵生产紫杉醇的潜力。

关键词: 南方红豆杉, 菌根菌, 紫杉醇, 10-去乙酰巴卡亭Ⅲ(10-DAB), 液体发酵

Abstract: To screen high-yield strains which could produce paclitaxel and 10-deacetylbaccation Ⅲ(10-DAB) from T.chinensis var.mairei mycorrhizal fungus,21 strains were separated, obtained and carried on liquid fermentation culture.The detection results of TLC,HPLC,and LC-MS show that 10 strains among those possess the ability to produce paclitaxel and taxane,and 10-DAB contents of 4 strains are 260.05, 92.88,393.27 and 70.52 μg/L,respectively.The paclitaxel contents of 6 strains are 434.94,1 242.19,200.65,360.77,317.90 and 29.82 μg/L among these 10 strains.These results indicate that the mycorrhizal fungus has the ability to synthesize paxlitaxel and taxanes, but each of the synthesized compounds have the difference. It is the different kinds of mycorrhizal that may participate in the taxol biosynthesis, and high contents of taxol result form collaborative actions. And there is the paclitaxel potential to produce in fermentation culture of the high-yield strains.

Key words: Taxus chinensis var.mairei, mycorrhizal fungus, paclitaxel, 10-deacetylbaccatin Ⅲ, liquid fermentation

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