高灰分麦糠水热预处理及其酶水解性能研究

doi:10.3969/j.issn.0253-2417.2019.05.004

林产化学与工业 ›› 2019, Vol. 39 ›› Issue (5): 26-32.doi: 10.3969/j.issn.0253-2417.2019.05.004

高灰分麦糠水热预处理及其酶水解性能研究

黄晨^1,2, 武新星², 赖晨欢², 黄曹兴², 李鑫², 勇强^1,2

1. 南京林业大学江苏省生物质绿色燃料与化学品重点实验室, 江苏南京 210037;
2. 南京林业大学化学工程学院, 江苏南京 210037

收稿日期:2019-04-06 出版日期:2019-10-25 发布日期:2019-11-01
通讯作者: 勇强,男,教授,博士生导师,研究领域为生物化工;E-mail:swhx@njfu.com.cn E-mail:swhx@njfu.com.cn
作者简介:黄晨(1991-),男,江苏泰州人,博士生,研究方向为生物质资源生物降解与转化
基金资助:
国家自然科学基金资助项目（31870569）

Liquid Hot Water Pretreatment of Waste Wheat Straw Containing High Ash Content

HUANG Chen^1,2, WU Xinxing², LAI Chenhuan², HUANG Caoxing², LI Xin², YONG Qiang^1,2

1. Jiangsu Key Lab of Biomass-based Green Fuels and Chemicals, Nanjing Forestry University, Nanjing 210037, China;
2. College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China

Received:2019-04-06 Online:2019-10-25 Published:2019-11-01

摘要/Abstract

摘要： 以高灰分含量麦糠（WWS）为原料，考察了水热预处理，以及预水洗后水热预处理对麦糠化学组分及其酶水解性能的影响。研究结果表明：麦糠在固液比1：10（g：mL）和180℃条件下水热预处理40 min，预处理麦糠的酶水解性能和酶解可发酵糖生成量最高，葡聚糖和木聚糖酶水解得率分别为40.84%和39.67%，可发酵糖生成量为15.74 g（其中葡萄糖11.68 g、木糖4.06 g）。进一步对预处理麦糠酶水解过程中酶用量进行优化，发现在纤维素酶用量40 FPU/g（以葡聚糖质量计）、木聚糖酶用量140 U/g（以木聚糖质量计）和β-葡萄糖苷酶用量48 U/g（以葡聚糖质量计）条件下，预处理麦糠葡聚糖和木聚糖酶水解得率可达最优值，分别为48.98%和49.06%。麦糠吸附型灰分的酸缓冲作用是制约其水热预处理效果的关键因素，预水洗可有效降低麦糠的灰分，同时提高葡聚糖和木聚糖含量；麦糠经洗涤比500：1（mL：g）预水洗后进行水热预处理，预处理麦糠的葡聚糖和木聚糖酶解得率分别从未水洗时的48.98%和49.06%提高到65.59%和70.11%，此时酶水解液中葡萄糖和木糖质量浓度分别可达17.50和4.75 g/L。同时，麦糠预水洗可有效降低后续酶解过程的纤维素酶用量。

关键词: 麦糠, 水热预处理, 预水洗, 可发酵糖

Abstract: The liquid hot water pretreatment (LHWP) of waste wheat straw (WWS) and the effect of prewashing on the LHWP were investigated. The pretreated WWS had the highest enzymatic hydrolysis and fermentable sugars production after the pretreatment at 180℃ for 40 min with the solid to liquid ratio of 1:10(g:mL). The corresponding glucan and xylan enzymatic hydrolysis yields were 40.84% and 39.67%, respectively, and the fermentation sugars production was 15.74 g (glucose was 11.68 g and xylose was 4.06 g). The yield of glucan and xylan were 48.98% and 49.06% respectively under the conditions of the cellulase, xylanase and β-glucosidase loadings of 40 FPU/g glucan, 140 U/g xylan and 48 U/g glucan. The key factor of the LHWP of WWS was the acid buffering ability of the absorbable ash in the WWS. Thus, the WWS was first water washed with the dose of 500 mL/g WWS and then was subjected to the LHWP. After prewashing, the enzymatic hydrolysis yields of glucan and xylan were increased from 48.98% and 49.06% to 65.59% and 70.11%, respectively, with the corresponding glucose and xylose concentrations of 17.50 and 4.75 g/L. Moreover, the prewashing could also lower the enzyme dosages in the enzymatic hydrolysis process.

Key words: waste wheat straw, liquid hot water pretreatment, prewashing, fermentable sugars

中图分类号:

TQ35

黄晨, 武新星, 赖晨欢, 黄曹兴, 李鑫, 勇强. 高灰分麦糠水热预处理及其酶水解性能研究[J]. 林产化学与工业, 2019, 39(5): 26-32.

HUANG Chen, WU Xinxing, LAI Chenhuan, HUANG Caoxing, LI Xin, YONG Qiang. Liquid Hot Water Pretreatment of Waste Wheat Straw Containing High Ash Content[J]. Chemistry and Industry of Forest Products, 2019, 39(5): 26-32.

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高灰分麦糠水热预处理及其酶水解性能研究

Liquid Hot Water Pretreatment of Waste Wheat Straw Containing High Ash Content

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