Abstract: The extraction conditions for subcritical fluid extraction of flavonoids from the cedar fallen pine needles were studied by orthogonal experimental design. In this paper, the total flavonoids content in pine needles was determined by UV Spectrophotometry. The contrast sample was rutin and the analyzed wavelength was 360 nm. The results showed that, the sequence of the impact of various factors on the extraction was as follows: extraction temperature >extraction time >liquid-material ratio >extraction pressure.The excellent levels of extraction of total flavonoids were as follows: extraction temperature 60 ℃, extraction pressure 4 MPa, extraction time 60 min, liquid-material ratio for the 3:1. Under these extraction conditions, the extraction amount of flavonoids rose up to 6.677 mg/g.

Key words: fallen pine needles, subcritical fluid, total flavonoids