自带介质血红密孔菌NFZH-1的鉴定及其木质素降解特性

doi:10.3969/j.issn.0253-2417.2014.05.001

Abstract

Abstract: The process of lignin degradation by white-rot fungi can be intensified by the presence of endogenous mediators. A high-yield laccase-producing fungus with an endogenous mediator is important for the commercial application of laccase. In the present study, we isolated two strains of Pycnoporus sanguineus, namely, NFZH-1 and NFZH-2 from mountain forest region in Nanjing, China, and their laccase production and delignifying characteristics was studied. We also studied these strains in comparison with the best-studied lignin-degrading fungus, Coriolus versicolor. P. sanguineus strain NFZH-1 with an endogenous mediator was identified. Thereafter, NFZH-1 was found to be a high yield laccase-producing strain as determined by the color-reaction and solid-state fermentation. The color zone diameter of NFZH-1 was found to be substantially greater than the mycelia colony one. Moreover, the color zone revealed a dark brown color-reaction using guaiacol as substrate. The laccase activities of P. sanguineus NFZH-1 were found to be 23 600 U/g, while no LiP and MnP were produced in solid-state fermentation. NFZH-1 demonstrated the capability to degrade 56.7% of Klason lignin and 36.6% of holocellulose in wheat straw within 30 days of incubation. Our results indicate that P. sanguineus NFZH-1 is a powerful and selective lignin-degrading strain.

Key words: Pycnoporus sanguineus, laccase, mediator, delignification, selectivity

CLC Number:

TQ35

FENG Nian-jie, ZHAI Hua-min, WANG Chuan-huai. Identification and Lignin Degradation of Pycnoporus sanguineus Strain NFZH-1 with an Endogenous Mediator[J]. Chemistry and Industry of Forest Products, 2014, 34(5): 1-7.

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Identification and Lignin Degradation of Pycnoporus sanguineus Strain NFZH-1 with an Endogenous Mediator

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