没食子单宁降解菌株的筛选及发酵条件优化

doi:10.3969/j.issn.0253-2417.2014.05.014

Chemistry and Industry of Forest Products ›› 2014, Vol. 34 ›› Issue (5): 84-90.doi: 10.3969/j.issn.0253-2417.2014.05.014

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Screening of Tannic Acid Degrading Strain and Optimization of Its Fermentation Conditions

MIN Fan-qin¹, WANG Cheng-zhang^1,2, YE Jian-zhong¹, LI Wen-jun¹, LU Li³

1. Institute of Chemical Industry of Forest Products, CAF;National Engineering Lab.for Biomass Chemical Utilization;Key and Open Lab.of Forest Chemical Engineering, SFA;Key Lab. of Biomass Energy and Material, Jiangsu Province, Nanjing 210042, China;
2. Research Institute of Forestry New Technology, CAF, Beijing 100091, China;
3. Hubei Academy of Forestry, Wuhan 430079, China

Received:2014-01-18 Online:2014-10-25 Published:2015-08-18
Contact: 王成章（1966-），男，湖北汉川人，研究员，博士生导师，主要从事天然产物研究与利用；E-mail：wangc_z@163.com E-mail:wangc_z@163.com

Abstract

Abstract: 3 strains of tannic acid degrading were screened out from the 11 strains which were separated from naturally fermented Turkish galls extract by the methods of spread-plate, streaking and colony observing,etc. One superior tannic acid degrading strain was found, named strain 1^#. The degradation rate of tannic acid reached beyond 65 %. In the fermentation broth, the concentration of gallic acid was 0.052 3 g/mL and the tannase activity was 0.920 U/mL. Fermentation conditions of strain 1^# were optimized by using Response Surface Method. The effect of temperature, initial pH, incubation time was investigated. The results showed that the optimum temperature, initial pH values and incubation time of strain 1^# was 31 ℃, 5 and 50 h. Under this condition, the tannase activity was increased from 0.920 to 1.170 U/mL, and improved by 27.2%.

Key words: tannic acid of Turkish galls, superior degrading strains, screening, optimization of fermentation conditions

CLC Number:

TQ35

MIN Fan-qin, WANG Cheng-zhang, YE Jian-zhong, LI Wen-jun, LU Li. Screening of Tannic Acid Degrading Strain and Optimization of Its Fermentation Conditions[J]. Chemistry and Industry of Forest Products, 2014, 34(5): 84-90.

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Screening of Tannic Acid Degrading Strain and Optimization of Its Fermentation Conditions

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