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Chemistry and Industry of Forest Products ›› 2020, Vol. 40 ›› Issue (2): 33-41.doi: 10.3969/j.issn.0253-2417.2020.02.004

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Preparation of Xylooligosaccharides by Ethanol Precipitation Separation from Autohydrolysis of Enzymatic Hydrolysis Residuce of Xylan

Zhina LIAN,Yan'e WANG,Jing LUO,Qiang YONG,Shiyuan YU*()   

  1. Key Laboratory of Forestry Genetics&Biotechnology, Ministry of Education; Jiangsu Co-innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China
  • Received:2019-10-24 Online:2020-04-28 Published:2020-04-27
  • Contact: Shiyuan YU E-mail:syu@njfu.edu.cn


Enzymatic hydrolysis residue of xylan was autohydrolyzed, and the autohydrolysate was then fractionated by two-step ethanol precipitation. The obtained three kinds of xylan fractions with varied degree of polymerization (Dp) were evaluated through in vitro proliferation by Bifidobacterium adolescentis. The autohydrolysis was carried out at 180℃ for 40 min to obtain autohydrolysate mainly contained xylooligosaccharides (XOS) with Dp of 2-6. The content of XOS was 57.36% of the xylan in the autohydrolysate, and the yield of XOS was 26.54% of the xylan in the feedstock. Two-step ethanol precipitation could fractionate the xylan in the autohydrolysate into sample S1, S2 and S3, with the range of Dp of 24-122, 7-19 and 1-6, respectively. Low Dp of sample S3 contained mainly XOS which was 95.91% and exhibited significant prebiotic activity. After 36 h incubation, the cell concentration was 0.25 g/L, 4.72 times of the initial, with the sugar residue of 43.48%, and with the concentration of short-chain fatty acids of 1.96 g/L.

Key words: xylooligosaccharide, autohydrolysis, degree of polymerization, ethanol precipitation, Bifidobacterium adolescentis

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