毛竹竹秆加压热水提取工艺优化及化学成分分析

doi:10.3969/j.issn.0253-2417.2023.04.015

Abstract

Abstract:

Taking moso bamboo stalks as raw material and the dry matter yield of moso bamboo stalks extract as the optimization index, the response surface methodology was used to optimize the process parameters of pressurized hot water extraction of compounds from bamboo stalks. The optimum extraction conditions were as follows: 4-year-old moso bamboo, extraction temperature of 165 ℃, extraction time of 30 min, solid-liquid ratio of 1∶12.5. Under these conditions, the dry matter yield was 12.44%, which was close to the predicted value(12.56%). The chemical composition of the pressurized hot water extract and the heating reflux water extract of moso bamboo stem was detected by gas chromatography-mass spectrometry(GC/MS). The results of GC/MS analysis showed that the chemical composition(content of 53.30%) of 23 kinds of pressurized hot water extracts from bamboo stems was much higher than that of 13 kinds of heated reflux water extracts(27.90%), and mainly contained active ingredients such as coniferaldehyde, vanillin, syringaldehyde, acetosyringone, 4-vinyl-2-methoxyphenol, 2, 3-dihydrobenzofuran and furfural.

Key words: moso bamboo stalks, pressurized hot water extraction, response surface methodology(RSD), yield of dry matter

CLC Number:

TQ35

Junhao YOU, Bao ZHANG, Hang XUN, Xi YAO, Jin WANG, Feng TANG. Optimization of Pressurized Hot Water Extraction Technology and Chemical Composition Analysis from Moso Bamboo Stalks[J]. Chemistry and Industry of Forest Products, 2023, 43(4): 107-114.

Figures/Tables 6

Fig.1

Table 1

Table 2

Fig.2

Fig.3

Table 3

Chemical composition of pressurized hot water extract and heating reflux water extract"

编号 No.	保留时间/min retention time	化合物 compound	分子式 molecular formula	相对峰面积relative peak area/%
编号 No.	保留时间/min retention time	化合物 compound	分子式 molecular formula	加压热水提取 pressurized hot water extraction	加热回流水提取 heating reflux water extraction
1	3.65	羟基乙酸甲酯methyl glycolate	C₃H₆O₃	0.18	0.31
2	4.53	丙酮酸甲酯methyl pyruvate	C₄H₆O₃	1.56	—
3	5.16	糠醛furfural	C₅H₄O₂	1.00	—
4	8.06	苯酚phenol	C₆H₆O	0.41	—
5	11.19	愈创木酚guaiacol	C₇H₈O₂	0.40	0.28
6	15.61	2, 3-二氢苯并呋喃2, 3-dihydrobenzofuran	C₈H₈O	17.25	5.81
7	18.75	4-乙烯基-2-甲氧基苯酚2-vinyl-4-methoxyphenol	C₉H₁₀O₂	7.38	2.98
8	20.33	2, 6-二甲氧基苯酚2, 6-dimethoxyphenol	C₈H₁₀O₃	1.05	0.34
9	22.66	香兰素vanillin	C₈H₈O₃	1.66	—
10	24.78	异丁香酚isoeugenol	C₁₀H₁₂O₂	0.75	0.31
11	25.06	2-甲氧基-4-丙基-苯酚2-methoxy-4-ropylphenol	C₁₀H₁₄O₂	0.38	—
12	25.77	4-羟基-3-甲氧基苯丙酮4-hydroxy-3-methoxyphenylacetone	C₁₀H₁₂O₃	0.73	—
13	32.11	丁香醛syringaldehyde	C₉H₁₀O₄	3.85	0.28
14	33.42	(E)-2, 6-二甲氧基-4-(丙-1-烯-1-基)苯酚 phenol, 2, 6-dimethoxy-4-(1E)-1-propen-1-yl-	C₁₁H₁₄O₃	—	1.64
15	34.11	1-羟基-3-(4-羟基-3-甲氧基苯基)丙烷-2-酮 1-hydroxy-3-(4-hydroxy-3-methoxyphenyl)-2-propane-2-one	C₁₀H₁₂O₄	1.07	—
16	34.12	4-羟基-3-甲氧基苯乙酸甲酯 methyl 4-hydroxy-3-methoxyphenylacetate	C₁₀H₁₂O₄	0.64	—
17	34.27	乙酰丁香酮acetosyringone	C₁₀H₁₂O₄	1.26	—
18	34.42	松柏醛4-hydroxy-3-methoxycinnamaldehyde	C₁₀H₁₀O₃	1.88	—
19	34.6	4-((1E)-3-羟基-1-丙烯基)-2-甲氧基苯酚 4-((1E)-3-hydroxy-1-propenyl)- 2-methoxyphenol	C₁₀H₁₂O₃	—	1.33
20	35.4	3, 5-二甲氧基-4-羟基苯酰肼 3, 5-dimethoxy-4-hydroxyphenylhydrazide	C₉H₁₂N₂O₄	0.56	—
21	36.66	二苯基-2, 2′-二甲醛biphenyl-2, 2′-dicarboxaldehyde	C₁₄H₁₀O₂	1.09	—
22	36.84	肉豆蔻酸异丙酯isopropyl myristate	C₁₇H₃₄O₂	0.86	1.83
23	38.39	棕榈酸甲酯methyl palmitate	C₁₇H₃₄O₂	—	1.30
24	38.80	二苯基乙酸甲酯methyl diphenylacetate	C₁₅H₁₄O₂	4.16	—
25	39.14	4-[(1E)-3-羟基-1-丙烯基]-2, 6-二甲氧基苯酚 4-[(1E)-3-hydroxy-1-propenyl]- 2, 6-dimethoxyphenol	C₁₁H₁₄O₄	5.02	8.90
26	39.32	反式-3, 5-二甲氧基-4-羟基肉桂醛 (E)-3-(4-hydroxy-3, 5-dimethoxyphenyl) acrylaldehyde	C₁₁H₁₂O₄	0.18	2.59

Table 3

References 21

1	中国造纸协会. 中国造纸协会关于造纸工业"十三五"发展的意见[J]. 中国造纸, 2017, 36 (7): 64- 69.
	China Paper Association . Opinions of the China paper association on the development of China's paper industry in the 13^th Five-Year Plan[J]. China Pulp & Paper, 2017, 36 (7): 64- 69.
2	李瑞丰, 贾锋伟, 李萌, 等. 竹浆造纸研究开发与实践[J]. 大科技, 2020, (19): 233- 235.
	LI R F , JIA F W , LI M , et al. Research, development and practice of bamboo pulp papermaking[J]. Super Science, 2020, (19): 233- 235.
3	樊永胜, 蔡忆昔, 李小华, 等. 樟木木屑真空热解工艺的响应面法优化及生物油组分分析[J]. 林产化学与工业, 2014, 34 (6): 29- 36.
	FAN Y S , CAI Y X , LI X H , et al. Vacuum pyrolysis of camphorwood sawdust optimized by response surface methodology and bio-oil composition analysis[J]. Chemistry and Industry of Forest Products, 2014, 34 (6): 29- 36.
4	崔敏. 毛竹竹龄对制浆性能的影响[D]. 北京: 北京林业大学, 2010.
	CUI M. Influence of bamboo ages on the pulping performance of Phyllostachys pubescens[D]. Beijing: Beijing Forestry University, 2010.
5	马晓娟. 竹材预水解碳水化合物溶出规律及其降解机制研究[D]. 福州: 福建农林大学, 2014.
	MA X J. Degradation and dissolution of carbohydrate during bamboo prehydrolysis[D]. Fuzhou: Fujian Agriculture and Forestry University, 2014.
6	任天宝, 马孝琴, 徐桂转, 等. 响应面法优化玉米秸秆蒸汽爆破预处理条件[J]. 农业工程学报, 2011, 27 (9): 282- 286. doi: 10.3969/j.issn.1002-6819.2011.09.049
	REN T B , MA X Q , XU G Z , et al. Optimizing steam explosion pretreatment conditions of corn stalk by response surface methodology[J]. Transactions of the Chinese Society of Agricultural Engineering, 2011, 27 (9): 282- 286. doi: 10.3969/j.issn.1002-6819.2011.09.049
7	SUN X X, ZHANG D, ZHAO L, et al. Antagonistic interaction of phenols and alkaloids in Sichuan pepper(Zanthoxylum bungeanum) pericarp[J/OL]. Industrial Crops and Products, 2020, 152: 112551[2022-03-20]. https://doi.org/10.1016/j.indcrop.2020.112551.
8	张慧, 郑璞, 陈鹏程. 微生物法转化丁香酚产松柏醛[J]. 食品与发酵工业, 2016, 42 (11): 82-85, 90.
	ZHANG H , ZHENG P , CHEN P C . Degradation of eugenol into coniferyl aldehyde by Gibberella strain ZH-34[J]. Food and Fermentation Industries, 2016, 42 (11): 82-85, 90.
9	MUSTHAFA S A , DABDOUB W , SADIQ M , et al. Syringaldehyde isolated from Abutilon indicum Linn. leaves exhibits broad spectrum anti-microbial activity[J]. Materials Today: Proceedings, 2022, 50, 335- 339. doi: 10.1016/j.matpr.2021.08.062
10	刘敏. 板栗挥发性香气成分研究[J]. 现代农业科技, 2020, (18): 203-205, 207.
	LIU M . Analysis on volatile aroma components in chestnut[J]. Modern Agricultural Science and Technology, 2020, (18): 203-205, 207.
11	高天元, 唐国琳, 吴情梅, 等. 鸡矢藤茎、叶及地上部分挥发油成分的GC-MS分析[J]. 时珍国医国药, 2020, 31 (6): 1294- 1297.
	GAO T Y , TANG G L , WU Q M , et al. GC-MS analysis of volatile oils from stems, leaves and aboveground parts of Paederia scandens[J]. Lishizhen Medicine and Materia Medica Research, 2020, 31 (6): 1294- 1297.
12	田晴, 孙立永, 杨胜广, 等. 不同品种甘薯烘烤后感官、质构及香气成分的差异[J]. 食品工业科技, 2021, 42 (5): 85- 92.
	TIAN Q , SUN L Y , YANG S G , et al. Differences of sensory, texture and aroma components of different sweet potato varieties after baking[J]. Science and Technology of Food Industry, 2021, 42 (5): 85- 92.
13	潘曦, 宋旭艳, 魏敏, 等. 加热非燃烧烟草薄片理化特性及热裂解性能研究[J]. 食品与机械, 2020, 36 (11): 39- 45.
	PAN X , SONG X Y , WEI M , et al. Study on physicochemical properties and pyrolysis properties of heated non combustion tobacco sheet[J]. Food & Machinery, 2020, 36 (11): 39- 45.
14	李明阳, 姚宇波, 徐翔, 等. 几种水稻对褐飞虱的抗性鉴定及抗性相关次生物质分析[J]. 应用昆虫学报, 2020, 57 (6): 1375- 1384.
	LI M Y , YAO Y B , XU X , et al. Relative resistance of several rice varieties to Nilaparvata lugens(Stal)and analysis of resistance-related secondary substances[J]. Chinese Journal of Applied Entomology, 2020, 57 (6): 1375- 1384.
15	DU C X , CHAI L , LIU C , et al. Improved Agrobacterium tumefaciens-mediated transformation using antibiotics and acetosyringone selection in cucumber[J]. Plant Biotechnology Reports, 2022, 16 (1): 17- 27.
16	刘凤. 乙酰丁香酮和香草醛对木聚糖酶的抑制作用及机制研究[D]. 广州: 南方医科大学, 2020.
	LIU F. Mechanisms and inhibitory effects of acetosyringone and vanillin on xylanase[D]. Guangzhou: Southern Medical University, 2020.
17	张海霞, 张少英, 付增娟. 乙酰丁香酮对甜菜遗传转化的影响[J]. 广东农业科学, 2013, 40 (22): 22- 24.
	ZHANG H X , ZHANG S Y , FU Z J . Effect of acetosyringone on the genetic transformation of sugarbeet[J]. Guangdong Agricultural Sciences, 2013, 40 (22): 22- 24.
18	宁宇, 李蕾, 苗永美, 等. 乙酰丁香酮及共培养pH对黄瓜遗传转化效率的影响[J]. 中国瓜菜, 2013, 26 (5): 6- 9.
	NING Y , LI L , MIAO Y M , et al. Influence of acetosyringone concentration and pH of co-culture medium on efficiency of cucumber genetic transformation[J]. China Cucurbits and Vegetables, 2013, 26 (5): 6- 9.
19	郭新梅, 车昕明, 裴玉贺, 等. 乙酰丁香酮对不同糯玉米受体系统遗传转化的影响[J]. 西南农业学报, 2013, 26 (3): 899- 902.
	GUO X M , CHE X M , PEI Y H , et al. Effect of acetosyringone on genetic transformation of receptor system of different waxy maizes[J]. Southwest China Journal of Agricultural Sciences, 2013, 26 (3): 899- 902.
20	黄荣清, 吴德雨, 骆传环, 等. 2, 3-二氢苯并呋喃的合成与分析[J]. 科学技术与工程, 2005, 5 (21): 1690- 1691.
	HUANG R Q , WU D Y , LUO C H , et al. The synthesis and qualitative analysis of 2, 3-dihydrobenzofuran[J]. Science Technology and Engineering, 2005, 5 (21): 1690- 1691.
21	PARK S , KANNAPU H P R , JEONG C , et al. Highly active mesoporous Cu-Al₂O₃ catalyst for the hydrodeoxygenation of furfural to 2-methylfuran[J]. ChemCatChem, 2020, 12 (1): 105- 111.

编号 No.	X₁ 竹龄/a bamboo age	X₂ 提取温度/℃ extraction temperature	X₃ 提取时间/min extraction time	X₄ 料液比(g∶mL) solid-liquid ratio	Y 干物质得率/% dry matter yield
1	3	155	30	1∶12.5	4.80±0.63
2	3	155	20	1∶10	5.88±0.22
3	3	165	20	1∶12.5	4.26±0.09
4	3	165	20	1∶7.5	6.72±0.45
5	2	155	20	1∶12.5	5.82±0.45
6	2	155	20	1∶7.5	11.70±0.46
7	2	155	10	1∶10	6.93±0.20
8	3	155	10	1∶7.5	7.14±0.36
9	3	145	10	1∶10	7.38±0.72
10	4	145	20	1∶10	6.24±0.02
11	2	155	30	1∶10	5.22±0.36
12	3	155	30	1∶7.5	4.98±0.48
13	4	155	20	1∶12.5	5.04±0.32
14	2	165	20	1∶10	6.84±0.87
15	3	155	20	1∶10	4.44±0.79
16	4	155	10	1∶10	9.60±0.29
17	3	145	20	1∶12.5	10.62±0.49
18	3	155	20	1∶10	5.31±0.05
19	3	155	10	1∶12.5	4.44±0.02
20	2	145	20	1∶10	6.18±0.57
21	3	145	20	1∶7.5	8.94±0.81
22	3	165	10	1∶10	6.33±0.02
23	3	165	30	1∶10	5.40±0.07
24	3	145	30	1∶10	4.68±0.34
25	4	155	30	1∶10	5.28±0.43
26	4	165	20	1∶10	5.70±0.17
27	4	155	20	1∶7.5	6.06±0.07

方差来源source of variation	平方和SS	自由度df	均方MS	F值F value	P值P value
模型model	83.05	14	5.93	7.6	0.000 6
X₁	1.43	1	1.43	1.83	0.201 2
X₂	49.09	1	49.09	62.86	< 0.000 1
X₃	6.53	1	6.53	8.36	0.013 5
X₄	0.39	1	0.39	0.5	0.493 9
X₁X₂	0.051	1	0.051	0.065	0.803 3
X₁X₃	1.37	1	1.37	1.75	0.210 1
X₁X₄	0.95	1	0.95	1.22	0.291 5
X₂X₃	3.24	1	3.24	4.15	0.064 3
X₂X₄	0.022	1	0.022	0.029	0.868 0
X₃X₄	0.19	1	0.19	0.24	0.631 4
X₁²	13.34	1	13.34	17.08	0.001 4
X₂²	3.65	1	3.65	4.68	0.051 5
X₃²	0.012	1	0.012	0.015	0.903 3
X₄²	0.41	1	0.41	0.53	0.480 3
残差residual	9.37	12	0.78
失拟项lock of fit	9.23	10	0.92	13.5	0.070 9
纯误差pure error	0.14	2	0.068
总和cor total	92.42	26

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Optimization of Pressurized Hot Water Extraction Technology and Chemical Composition Analysis from Moso Bamboo Stalks

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