Abstract: Effective extraction of flavones from persimmon leaves is an important technique in bioresearch. In the present study, extraction and purification of flavones of persimmon leaves were carried out simultaneously by using an aqueous partitioning method. This two-phase aqueous system was prepared by dissolving suitable amounts of polyethylene glycol(PEG)and(NH₄)₂SO₄ in aqueous solution. Flavones of persimmon leaves were recovered with optimum high yield and high concentration in the upper polymer-rich phase when 25% PEG 600and25% (NH₄)₂SO₄ were applied. The optimum extraction conditions were pH value 11, MgCl₂ 3% and temperature 25℃.

Key words: flavones of persimmon leaves, polyethylene glycol, two-phase aqueous system