Abstract: The possibility of using high-performance capillary electrophoresis (HPCE) to determine azadirachtin A was analysed theoretically, and proved experimentally.The stable current curve had been obtained under the testing condition as follows: SDS concentration 25 mmol/L, phosphate buffer solution concentration 0.02 mol/L, pH value 6.86, volume ratio of methanol-buffer solution 7:3, separation voltage 20 kV. The characteristic electrophoretogram of azadirachtin A was very well examinated by PDA at wavelengh 214 nm and migration time 2.6 min. The result of area integration has no notable difference with that of HPLC.

Key words: high performance capillary electrophoresis, azadirachtin A, determination, buffer solution