响应面法优化微波辅助提取成熟叶老鹰茶总黄酮及其抗氧化研究

doi:10.3969/j.issn.0253-2417.2014.03.015

Abstract

Abstract: The optimal microwave-assisted extraction conditions of total flavonoids from matured leaf Hawk tea were studied. In order to increase the extraction yield of total flavonoids, the microwave-assisted extraction conditions of total flavonoids from matured leaf Hawk tea were optimized by response surface methodology based on the single-factor tests. Meanwhile, the antioxidant activity of total flavonoids from matured leaf Hawk tea was evaluated by the DPPH radical scavenging capacity and ferric reducing activity power. The optimal parameters of total flavonoids were matured leaf Hawk tea 1 g, microwave time 61 s, microwave power 560 W, ratio of water to solid 10:1 (mL:g), ethanol concentration 80.3%. The maximum extraction yield of total flavonoids was 6.52%. At 20 g/L, DPPH radical scavenging activity and FRAP value of extracted total flavonoids were 66.66% and 2.05 mmol/L FeSO₄, respectively. They all showed a dose-dependent manner.

Key words: Hawk tea, microwave-assisted extraction, response surface methodology, total flavonoids

CLC Number:

TQ35
TS202.1

JIA Xue-jing, LI Le, DING Chun-bang, DU Lei, YUAN Shu, YUAN Ming. Optimization of Microwave-assisted Extraction of Total Flavonoids from Matured Leaf Hawk Tea by Response Surface Methodology and Its Antioxidant Activity[J]. Chemistry and Industry of Forest Products, 2014, 34(3): 85-91.

References

[1] JIA Xue-jing,DONG Li-hua,YANG Yong,et al.Preliminary structural characterization and antioxidant activities of polysaccharides extracted from Hawk tea (Litsea coreana var.lanuginosa)[J].Carbohydrate Polymers,2013,95(1):195-199.
[2] 向应海,鲁新成.老鹰茶——贵州大娄山民族民间古茶种[J].贵州科学,1998,16(3):216-220.
[3] 郁建平,古练权.贵州老鹰茶的化学成分[J].植物资源与环境学报,2001,10(3):61-62.
[4] TSAO R,YANG R.Optimization of a new mobile phase to know the complex and real polyphenolic composition:Towards a total phenolic index using high-performance liquid chromatography[J].Journal of Chromatography A,2003,1018(1):29-40.
[5] LIU Qian,CAI Wen-sheng,SHAO Xue-guang.Determination of seven polyphenols in water by high performance liquid chromatography combined with preconcentration[J].Talanta,2008,77(2):679-683.
[6] 叶辉,郁建平.老鹰茶中三种黄酮类物质抗脂质过氧化作用初探[J].中药材,2004,27(2):113-115.
[7] 吕雄文,李俊,邹宇宏,等.老鹰茶总黄酮降血糖作用的实验研究[J].中国中医药科技,2008,15(2):119-121.
[8] WANG Ting-yu,LI Jun,GE Jin-fang,et al.Preliminary study of total flavonoids from Litsea coreana Levl.on experimental adjuvant-induced arthritis in rats[J].The American Journal of Chinese Medicine,2008,36(5):899-912.
[9] 李廷松.老鹰茶资源调查与开发利用[J].贵州茶叶,1995(4):10-13.
[10] 张蕾蕾,常雅宁,夏鹏竣,等.微波法提取紫苏黄酮类物质及其成分分析[J].食品科学,2012,33(22):53-57.
[11] 向昌国,向宁,黄成龙,等.响应面法优化刺槐花黄酮类化合物的微波提取工艺[J].食品科学,2011,32(22):32-36.
[12] 尹波,王科军,钟金莲,等.微波和超声波提取脐橙皮渣总黄酮工艺条件优化[J].食品科学,2012,33(6):19-23.
[13] 杨晓凤.酶法提取老鹰茶中黄酮类化合物[J].西南农业学报,2012,24(6):2369-2371.
[14] 计红芳,南海娟,张令文,等.老鹰茶总黄酮的提取工艺及抗氧化活性研究[J].中国食品添加剂,2011(2):121-125.
[15] 侯学敏,李林霞,张直峰,等.响应面法优化薄荷叶总黄酮提取工艺及抗氧化活性[J].食品科学,2013,34(6):124-128.
[16] HUANG De-jian,OU Bo-xin,PRIOR R L.The chemistry behind antioxidant capacity assays[J].Journal of Agricultural and Food Chemistry,2005,53(6):1841-1856.
[17] 陈玉霞,刘建华,林峰,等.DPPH和FRAP法测定41种中草药抗氧化活性[J].实验室研究与探索,2011,30(6):11-14.
[18] RAZALI N,RAZAB R,JUNIT S M,et al.Radical scavenging and reducing properties of extracts of cashew shoots (Anacardium occidentale)[J].Food Chemistry,2008,111(1):38-44.
[19] VASCO C,RUALES J,KAMAL-ELDIN A.Total phenolic compounds and antioxidant capacities of major fruits from Ecuador[J].Food Chemistry,2008,111(4):816-823.
[20] 王迪,王伟,牟洪举,等.响应面法优化松茸菌丝体多糖超声波提取工艺及其抗氧化研究[J].林产化学与工业,2009,29(5):109-114.
[21] 秦清,闵凡芹,徐浩,等.响应面法优化微波辅助提取五倍子单宁工艺研究[J].林产化学与工业,2012,32(6):84-88.
[22] 侯学敏,李林霞,张直峰,等.响应面法优化薄荷叶总黄酮提取工艺及抗氧化活性[J].食品科学,2013,34(6):125-128.

Optimization of Microwave-assisted Extraction of Total Flavonoids from Matured Leaf Hawk Tea by Response Surface Methodology and Its Antioxidant Activity

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	WANG Xiaojie, HUANG Lixin, ZHANG Caihong, XIE Pujun, DENG Yejun, LIU Lujie. Preparation and Physicochemical Properties of Olive Leaf Extract [J]. Chemistry and Industry of Forest Products, 2019, 39(4): 83-90.
[2]	LIU Dandan, HAN Weifan, JIANG Jianxin, ZHU Liwei. Technology of Microwave-assisted Extraction of Sapindus Peel Polysaccharide and Its Antioxidant Activity [J]. Chemistry and Industry of Forest Products, 2018, 38(4): 109-116.
[3]	JIANG Ping, CHEN Huafang, HUANG Haibin, BAO Xinye, XU Lan. Ultrasonic-assisted Extraction of Total Flavonoids from Branches and Leaves of Taxus cuspidata Sieb. et Zucc. and Its Antioxidant Activity [J]. Chemistry and Industry of Forest Products, 2017, 37(6): 125-132.
[4]	LIU Sha, CAI Yi-xi, FAN Yong-sheng, LI Xiao-hua, WANG Jia-jun. Optimization of Online Upgrading of Bio-oil with MCM-41 and Catalyst Durability Analysis [J]. Chemistry and Industry of Forest Products, 2016, 36(4): 55-63.
[5]	ZHONG Fang-li, WANG Wen-jiao, WANG Xiao-lin, CHEN Ye, LUO Ya-hong. Aqueous Two-phase Extraction of Total Flavonoids in Fruit of Rosa davurica Pall. and Its Antioxidant Ability [J]. Chemistry and Industry of Forest Products, 2016, 36(4): 64-72.
[6]	XU Xiao-xiang, YIN Xiao-li, HONG Yan-ping, ZOU Kai, SHANGGUAN Xin-chen, LIANG Qiang. Extraction Process and Antioxidant Activity of Pinus taeda L. Polysaccharides [J]. Chemistry and Industry of Forest Products, 2016, 36(3): 114-120.
[7]	LIU Tong-fang, YU Hua-zhong, CHEN Yan-mei, WANG Ting. Optimization of Semisynthetic Myricetin from Dihydromyricetion by Response Surface Process [J]. Chemistry and Industry of Forest Products, 2015, 35(4): 125-130.
[8]	TIAN Ning, XIAN Mo, HU Yang-dong, LIU Wei. Optimization of Medium for Production of Geraniol by the Recombinant Escherichia coli [J]. Chemistry and Industry of Forest Products, 2015, 35(4): 131-137.
[9]	LI He, LIN Ri-hui, HUANG Wen-qin, LONG Han, LIU Zuo-jiao, SU Jia-ting. Catalytic Synthesis of Rosin Starch Ester by Immobilized Lipase [J]. Chemistry and Industry of Forest Products, 2015, 35(2): 103-110.
[10]	FAN Yong-sheng, CAI Yi-xi, LI Xiao-hua, YU Ning, ZHANG Rong-xian, YIN Hai-yun. Vacuum Pyrolysis of Camphorwood Sawdust Optimized by Response Surface Methodology and Bio-oil Composition Analysis [J]. Chemistry and Industry of Forest Products, 2014, 34(6): 29-36.
[11]	XU Chun-ming, WANG Ying-ying, LI Ting, PANG Gao-yang, LI Dan. Microwave-assisted Extraction and Bioavailability of Flavonoids from Ginkgo Leaves [J]. Chemistry and Industry of Forest Products, 2014, 34(4): 131-136.
[12]	ZHU Jun-jun, ZHU Yuan-yuan, CHENG Ting, XU Yong, YONG Qiang, YU Shi-yuan. Effects of Hydrolyzate of Corn Stover Acetyl Groups on Ethanol Fermentation and Its Removal Strategy [J]. Chemistry and Industry of Forest Products, 2014, 34(1): 1-7.
[13]	FAN Yong-sheng, CAI Yi-xi, LI Xiao-hua, ZHAO Wei-dong, YU Ning. Influence of Process Parameters on Bio-oil Yield by Vacuum Pyrolysis [J]. Chemistry and Industry of Forest Products, 2014, 34(1): 79-85.
[14]	HAN Dong;WANG Jue;ZHU Xing-yi;XIE Jie. Homogenate Extraction of Polysaccharides from Gardenia jasminoides Ellis by Response Surface Methodology [J]. , 2013, 33(6): 59-62.
[15]	LU Sheng-lou;LIU Hong;ZANG Wen-xia;LI Yun;ZHANG Qiang;WANG Zi-huan. Optimization of Ultrasound Extraction of Total Triterpenoids from Synsepalum dulcificum Leaf by Response Surface Methodology [J]. , 2013, 33(6): 95-100.