希腊海岸松叶中聚戊烯醇含量与化学结构分析

doi:10.3969/j.issn.0253-2417.2014.05.025

Chemistry and Industry of Forest Products ›› 2014, Vol. 34 ›› Issue (5): 152-156.doi: 10.3969/j.issn.0253-2417.2014.05.025

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Determination of the Content and Structure of Polyprenols from Greek Pinus pinaster Leaves

DUAN Xin^1,2, TAO Ran¹, WANG Cheng-zhang¹, XUE Zhi-ge^2,3

1. Institute of Chemical Industry of Forest Products, CAF;National Engineering Lab.for Biomass Chemical Utilization;Key and Open Lab.of Forest Chemical Engineering, SFA;Key Lab.of Biomass Energy and Material, Jiangsu Province, Nanjing 210042, China;
2. Yunnan University of Traditional Chinese Medicine, Kunming 650500, China;
3. Yunnan Province Food and Drug Administration, Kunming 650000, China

Received:2012-11-13 Revised:2014-06-12 Online:2014-10-25 Published:2015-08-18
Contact: 王成章（1966-），男，湖北汉川人，研究员，博士生导师，主要从事天然产物研究与利用；E-mail：wangczlhs@sina.com E-mail:wangczlhs@sina.com

Abstract

Abstract: The isopentenyl unit numbers of polyprenols from Greek Pinus pinaster leaves was 14 to 20 by contrasting the retention time of polyprenol reference substance using HPLC. And the polyprenols structure was confirmed as ω-trans₂-cis_n-OH by ¹H and ¹³C spectrum. The HPLC conditions of a Kromasil C18 ODS-1(150 mm × 4.6 mm, 5 μm) column, methanol-isopropanol (9:16, v/v) as the mobile phase at a flow rate of 0.50 mL/min, column temperature of 25 ℃, PDA detector with detective wavelength of 210 nm were used. The polyprenols from Greek P. pinaster leaves in the range of 1.10-12.10 μg had good linearity (r=0.996 3), the average recovery was 98.21 %, and RSD was 2.27 % (n=6). The result showed that the polyprenols content of dried leaves was 0.33 % . The method was accurate, reproducible and quick to determine the isopentenyl unit number and content of polyprenols from Greek P. pinaster leaves.

Key words: Pinus pinaster, polyprenols, chemical structure, isopentenyl unit number

CLC Number:

TQ35

DUAN Xin, TAO Ran, WANG Cheng-zhang, XUE Zhi-ge. Determination of the Content and Structure of Polyprenols from Greek Pinus pinaster Leaves[J]. Chemistry and Industry of Forest Products, 2014, 34(5): 152-156.

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Determination of the Content and Structure of Polyprenols from Greek Pinus pinaster Leaves

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